Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,489 G→T 17.2% intergenic (+301/‑630) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4890G→T17.2% 104.6 ‑0.3 ‑0.0242intergenic (+301/‑630)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/19):  ref base (102/98):  total (125/117)
Fisher's exact test strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395390‑1395589
                                                                                                   |                                                                                                    
tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                                                                                     <  2:2984023/101‑1 (MQ=255)
 cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                                                                                    >  1:1286704/1‑101 (MQ=255)
   aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                                                                                  >  1:2896230/1‑101 (MQ=255)
   aaaCTCCACCCCGTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                                                                                  <  2:909000/101‑1 (MQ=255)
    aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                                                                                 <  2:4587914/101‑1 (MQ=255)
     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                                                >  2:3229170/1‑101 (MQ=255)
       tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                                              <  1:4260757/101‑1 (MQ=255)
       tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                                              <  1:4413331/101‑1 (MQ=255)
       tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                                              >  2:3542194/1‑101 (MQ=255)
       tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGCCGACAGGATGGTAAc                                                                                              >  2:167793/1‑101 (MQ=255)
          ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtg                                                                                           <  1:2342844/100‑1 (MQ=255)
           cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                                                                                          <  2:2888007/101‑1 (MQ=255)
            ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                                         >  1:4596619/1‑101 (MQ=255)
               cgggCCCTGCTGGTGATCGGTGATCAGCGGTCTCAACGCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                                      <  2:1333544/99‑1 (MQ=38)
                tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                     >  2:20757/1‑101 (MQ=255)
                tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                                     >  1:924648/1‑101 (MQ=255)
                   cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                                  <  1:3033837/101‑1 (MQ=255)
                    ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                                                 <  1:1791516/101‑1 (MQ=255)
                     cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                                <  2:939740/101‑1 (MQ=255)
                      tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                               >  2:2494859/1‑101 (MQ=255)
                        ccGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgcg                                                                             <  2:3344091/101‑1 (MQ=255)
                         cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                                            >  2:1593180/1‑101 (MQ=255)
                         cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                                            <  2:2759015/101‑1 (MQ=255)
                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                                          >  1:2676108/1‑101 (MQ=255)
                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGAATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTGGCTCCCCCCGCgggg                                                                          <  2:513542/101‑1 (MQ=255)
                            tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCggggg                                                                         >  2:2778338/1‑101 (MQ=255)
                              atcggtgatcAGCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                                       <  1:4364754/101‑1 (MQ=255)
                               tcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt                                                                      <  2:4549414/101‑1 (MQ=255)
                               tcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt                                                                      <  2:4051398/101‑1 (MQ=255)
                                cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                                     >  2:3719644/1‑101 (MQ=255)
                                 cgctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   <  1:1485202/91‑1 (MQ=2)
                                 cgctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   >  1:1953227/11‑101 (MQ=2)
                                  gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGac                                                                  >  1:4057952/10‑100 (MQ=2)
                                  gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  >  1:3650513/10‑101 (MQ=2)
                                   tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:891739/101‑1 (MQ=255)
                                   tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:3900524/101‑1 (MQ=255)
                                   tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGgggt                                                                  >  2:1759181/1‑99 (MQ=255)
                                   ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:2395840/93‑1 (MQ=0)
                                   ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGATCCCCCCGCGGGGGCGTGGATc                                                                 >  2:3479878/9‑101 (MQ=2)
                                    gatcAGCGGTCGCTCCCCCCGCGGGGGCGGNGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  1:438244/101‑1 (MQ=255)
                                     ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               >  2:1984671/7‑101 (MQ=2)
                                     atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:1456295/101‑1 (MQ=255)
                                       cAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:714163/1‑101 (MQ=255)
                                       acgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:622734/97‑1 (MQ=2)
                                        cgtgtgcaccccccccGCGGGGGCGTGGATCGAAACTACGCCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  2:2539883/91‑1 (MQ=2)
                                        cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  1:273645/98‑1 (MQ=2)
                                         gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           <  1:472245/99‑1 (MQ=2)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                                         >  2:2488923/1‑99 (MQ=2)
                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          <  2:3591163/101‑1 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                                       >  1:3671143/1‑100 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                     <  2:2351041/101‑4 (MQ=2)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGT.TACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                     >  1:1533124/1‑98 (MQ=0)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                      >  1:655815/1‑101 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                                    <  1:554453/101‑5 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                     <  1:3479402/101‑1 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCCTGGATCGAAACATCTCgg                                                     >  2:796291/1‑101 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                                                  >  2:1943675/1‑95 (MQ=0)
                                                  ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                                   >  1:2188082/1‑101 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                                   <  1:4032220/101‑1 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                                >  1:3929405/1‑93 (MQ=2)
                                                    ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                                <  1:4555516/101‑9 (MQ=2)
                                                     cccGTGGGGGCGTGGATCGAAATATCGCCGCTTTGGCGGTCGCCAGGCTGGTAACGTGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                <  2:4391808/101‑1 (MQ=38)
                                                      ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                               >  1:3353402/1‑101 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCg                                             <  2:3650410/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                            >  1:3435219/1‑101 (MQ=255)
                                                          gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                           <  1:2495025/101‑1 (MQ=255)
                                                          gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                           >  2:3386569/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                                          <  2:2280328/101‑1 (MQ=255)
                                                            gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCg                                         >  2:4653729/1‑101 (MQ=255)
                                                             ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGt                                        <  2:1782558/101‑1 (MQ=255)
                                                               cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                                      >  1:3868931/1‑101 (MQ=255)
                                                               cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGCcc                                      >  1:1158353/1‑101 (MQ=255)
                                                               cGTCGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                                      >  2:311519/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCTGTGCCGCCGCTCGTCCa                                     <  2:3877787/101‑1 (MQ=255)
                                                                gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                     >  2:1519881/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                                     <  1:2966163/101‑1 (MQ=255)
                                                                 tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                                    <  1:204784/101‑1 (MQ=255)
                                                                 tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                                    >  2:600199/1‑101 (MQ=255)
                                                                  ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                                   >  1:4643952/1‑101 (MQ=255)
                                                                     tCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCgg                                >  1:4320464/1‑101 (MQ=255)
                                                                      cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                               >  1:3551084/1‑101 (MQ=255)
                                                                       gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc                              >  2:1424380/1‑101 (MQ=255)
                                                                         aaCATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg                            >  1:4133367/1‑101 (MQ=255)
                                                                           cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt                          <  2:2693202/101‑1 (MQ=255)
                                                                           cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTCCGTGCCGTAGCTCGTCCCGCCTGCCTgtt                          >  2:3441500/1‑99 (MQ=37)
                                                                            aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc                         >  2:1108139/1‑101 (MQ=255)
                                                                             tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcg                        <  2:2102500/101‑1 (MQ=255)
                                                                              cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt                       <  1:3152431/101‑1 (MQ=255)
                                                                              cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt                       <  1:974191/101‑1 (MQ=255)
                                                                                 cgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGc                    >  1:424543/1‑101 (MQ=255)
                                                                                   cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc                  <  2:4300622/101‑1 (MQ=255)
                                                                                        gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccccc             >  2:1489786/1‑101 (MQ=255)
                                                                                        gCGGTCCACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccccc             <  2:714088/101‑1 (MQ=255)
                                                                                             cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg        >  2:4240508/1‑101 (MQ=255)
                                                                                             cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg        >  1:2250040/1‑101 (MQ=255)
                                                                                             cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg        >  1:1304252/1‑101 (MQ=255)
                                                                                             cGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCggg        <  1:508448/101‑1 (MQ=255)
                                                                                                cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGc     >  1:4217316/1‑101 (MQ=255)
                                                                                                cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGc     <  1:2556100/101‑1 (MQ=255)
                                                                                                  ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGt   <  2:1225069/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
TCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395390‑1395589

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG