Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,668 T→C 5.5% intergenic (+480/‑451) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6680T→C 5.5% 19.8 ‑1.9 ‑0.0237intergenic (+480/‑451)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (134/89):  total (140/97)
Fisher's exact test strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.88e-01

Only 100 of 258 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395569‑1395768
                                                                                                     |                                                                                                      
cgCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTg                                                                                                       >  2:2301745/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                    >  2:3596065/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                    >  1:2334190/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCCTg                                                                                                   >  2:4353258/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                   >  2:2759318/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                   >  2:1104328/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                   <  1:4189995/101‑1 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTCACGGT.TTGGGGCGGA..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCtttcaatg                                                                                                 <  2:2867668/101‑8 (MQ=2)
     cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                  <  1:1019182/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCCCCTTGCGGCT.T.GTCACTTCCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                <  2:593523/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                <  1:3178283/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                               >  1:3488789/1‑101 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                               <  2:3507559/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGAGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAACACCTGCAGTTAGt                                                                                               >  2:3689276/1‑101 (MQ=38)
         cgcgGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                              >  1:1436795/1‑101 (MQ=255)
            gggggCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggc                                                                                           <  1:2171506/101‑1 (MQ=255)
             ggggCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                          <  2:3389475/101‑1 (MQ=255)
                gCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.Gc                                                                                      <  2:3128638/101‑1 (MQ=255)
                  gTGGATCGAAACCCCCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAg                                                                                    <  2:1988671/101‑1 (MQ=255)
                   tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   <  1:787559/101‑1 (MQ=255)
                   tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   >  1:2102832/1‑101 (MQ=255)
                   tGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGt                                                                                   >  2:1719699/1‑101 (MQ=255)
                    ggATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                  <  2:2896014/101‑1 (MQ=255)
                    ggATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGtt                                                                                  <  1:1249426/101‑1 (MQ=255)
                     gATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTc                                                                                 <  1:3398163/101‑1 (MQ=255)
                      aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGGGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                >  1:4130328/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                >  1:4392892/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  2:1210393/101‑1 (MQ=255)
                      aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                >  1:1378243/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                >  2:4313303/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCg                                                                                <  1:1759300/101‑1 (MQ=255)
                        cGAGACCACATTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGGGGAGCGAAACAGCTGCACGCAGTCGGCGC.GCCGCTCGA.g                                                                             >  2:162314/1‑101 (MQ=25)
                         gAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            <  1:2759516/101‑1 (MQ=255)
                         gAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            >  1:2704004/1‑101 (MQ=255)
                         gAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            >  2:217532/1‑101 (MQ=255)
                         gAAACCACCTTGCGGAT.T.GTCACGTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gc                                                                            <  2:655753/101‑1 (MQ=255)
                          aaaCCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gcc                                                                           >  2:2451416/1‑101 (MQ=255)
                          aaaCCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCACTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.Gcc                                                                           >  2:943537/1‑101 (MQ=255)
                             ccaccTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCa                                                                        <  2:4232643/101‑1 (MQ=255)
                              caccTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAg                                                                       >  1:2184386/1‑101 (MQ=255)
                                ccTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTc                                                                     <  1:3596333/101‑1 (MQ=255)
                                 cTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCg                                                                    <  2:3550826/101‑1 (MQ=255)
                                  ttGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGc                                                                   >  2:1897122/1‑101 (MQ=255)
                                   tGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCt                                                                  <  1:4413393/101‑1 (MQ=255)
                                    gCGGAT.T.GTCCCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTc                                                                 <  1:93345/101‑1 (MQ=255)
                                       gAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                              >  1:3339632/1‑101 (MQ=255)
                                       gAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                              >  1:4092808/1‑101 (MQ=255)
                                       gAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                              >  2:4133052/1‑101 (MQ=255)
                                       gAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTcccc                                                              >  2:465538/1‑101 (MQ=255)
                                        gt.t.GTCACTTTCGCGGGGCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTccccc                                                             <  2:874945/100‑1 (MQ=255)
                                        aT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTccccc                                                             <  1:1519990/101‑1 (MQ=255)
                                             gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                          >  1:1189058/1‑101 (MQ=255)
                                               cACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                        >  1:525253/1‑101 (MQ=255)
                                                ccTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggg                                                       <  1:1624763/100‑1 (MQ=255)
                                                aCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggg                                                       >  1:4258449/1‑101 (MQ=255)
                                                  tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                     >  2:3945711/1‑101 (MQ=255)
                                                     cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGT.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                  <  2:1151018/101‑1 (MQ=255)
                                                     cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCgg                                                  >  2:2735204/1‑100 (MQ=255)
                                                     cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                  >  1:2213866/1‑101 (MQ=255)
                                                       cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                >  1:1708800/1‑101 (MQ=255)
                                                            cGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                           <  1:374188/101‑1 (MQ=255)
                                                             gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          >  1:3120485/1‑101 (MQ=255)
                                                              ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:4626361/99‑1 (MQ=2)
                                                              ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:1510206/99‑1 (MQ=2)
                                                               tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  2:2043302/2‑101 (MQ=2)
                                                               tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCGGCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  2:3025890/2‑101 (MQ=2)
                                                               aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:3471186/1‑101 (MQ=255)
                                                                 cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                      >  2:489493/1‑101 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACTgg                                     >  1:2042608/1‑101 (MQ=255)
                                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GACGCAGGCGCTTCCCCCGCGGGGGGGTGGATAGAAAATgg                                     >  2:2704886/1‑101 (MQ=37)
                                                                  gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                     <  1:190855/101‑1 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                  <  1:329752/101‑1 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                               <  1:2870864/101‑1 (MQ=255)
                                                                             cgggggggGGTGGATCGAAACATCTGCATGTAGTCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                          <  2:3200289/98‑1 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACATGTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                         >  2:4599516/1‑101 (MQ=255)
                                                                              gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGGTAGGGATCTGgc                         >  2:374939/1‑101 (MQ=255)
                                                                               ggggggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                        <  2:221146/100‑1 (MQ=255)
                                                                                   ggCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                    <  1:85685/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                   <  1:3781842/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                   >  1:2491245/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                 >  2:4544386/1‑101 (MQ=255)
                                                                                      gTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                 >  2:1390499/1‑101 (MQ=255)
                                                                                       tGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                >  2:1419203/1‑101 (MQ=255)
                                                                                       tGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                <  1:353206/101‑1 (MQ=255)
                                                                                       tGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                >  2:3711471/1‑101 (MQ=255)
                                                                                        ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               >  2:4207078/1‑101 (MQ=255)
                                                                                        ggATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa               >  1:4435724/1‑101 (MQ=255)
                                                                                         gATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              <  2:389278/101‑1 (MQ=255)
                                                                                         gATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa              >  1:196784/1‑101 (MQ=255)
                                                                                          aTCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg             <  1:3653671/101‑1 (MQ=255)
                                                                                           tCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt            >  1:259671/1‑101 (MQ=255)
                                                                                           tCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt            >  2:2665598/1‑101 (MQ=255)
                                                                                            cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           <  1:2270786/101‑1 (MQ=255)
                                                                                            cGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc           >  2:2500292/1‑101 (MQ=255)
                                                                                             gAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          <  1:1056151/101‑1 (MQ=255)
                                                                                             gAAACATCGGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt          <  1:2778536/101‑1 (MQ=255)
                                                                                                 cATCTGCATGTGGGCGGGGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg      <  1:397770/101‑1 (MQ=255)
                                                                                                 cATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTcagatc      >  1:2410716/1‑96 (MQ=255)
                                                                                                  ctCTGGATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt     <  2:761454/100‑1 (MQ=255)
                                                                                                   tCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc    <  2:4617803/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAT.T.GTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGG.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGC  >  NC_014034/1395569‑1395768

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG