Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,669 G→C 5.5% intergenic (+481/‑450) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6690G→C 5.5% 20.3 ‑1.9 ‑0.1236intergenic (+481/‑450)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/8):  ref base (135/88):  total (140/96)
Fisher's exact test strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.31e-01

Only 100 of 258 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCT  >  NC_014034/1395569‑1395769
                                                                                                    |                                                                                                        
   tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                       >  1:3520281/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                       >  1:2334190/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCAt                                                                                                       >  2:3596065/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCCTg                                                                                                      >  2:4353258/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATg                                                                                                      >  2:2034406/1‑101 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGt                                                                                                     <  1:1019182/101‑1 (MQ=255)
       cccGCGGGGGCGTGGATCGAAACCCCCTTGCGGCTTGTCACTTCCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                   <  2:593523/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                  <  2:3507559/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGAGTCGCTCCCCCCGCGGGGGGGGGGGTCGAAACACCTGCAGTTAGt                                                                                                  >  2:3689276/1‑101 (MQ=38)
         cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                                 >  1:2774088/1‑101 (MQ=255)
         cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                                 >  1:1436795/1‑101 (MQ=255)
           cgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcgg                                                                                               >  1:389315/1‑101 (MQ=255)
            gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggc                                                                                              <  1:2171506/101‑1 (MQ=255)
             ggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                             <  1:3386822/101‑1 (MQ=255)
             ggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                             <  2:3389475/101‑1 (MQ=255)
                gCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.Gc                                                                                       <  2:3128638/101‑1 (MQ=255)
                 cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCa                                                                                      <  2:2249878/101‑1 (MQ=255)
                  gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGAGGGGGGGTGGATCGAAACATCTTCATGTAGTCGGCG..G.GCAg                                                                                     >  1:4563160/1‑101 (MQ=255)
                   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGt                                                                                    >  1:2102832/1‑101 (MQ=255)
                   tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGt                                                                                    <  1:796365/101‑1 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGtt                                                                                   <  2:2896014/101‑1 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGtt                                                                                   <  1:1249426/101‑1 (MQ=255)
                    ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCANGTAGTCGGCG..G.GCAGtt                                                                                   >  2:1402289/1‑101 (MQ=255)
                     gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTc                                                                                  >  2:2078314/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCg                                                                                 <  1:1759300/101‑1 (MQ=255)
                      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCg                                                                                 >  2:4313303/1‑101 (MQ=255)
                      aTCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCg                                                                                 >  1:1378243/1‑101 (MQ=255)
                       tCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGCAGACGGCG..G.GCAGTTCGa                                                                                >  2:1339793/1‑101 (MQ=38)
                        cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.g                                                                              >  2:2187030/1‑101 (MQ=255)
                         gAAACCACCTTGCGGATTGTCACGTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gc                                                                             <  2:655753/101‑1 (MQ=255)
                          aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCACTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.Gcc                                                                            >  2:943537/1‑101 (MQ=255)
                             ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCa                                                                         <  2:4232643/101‑1 (MQ=255)
                              caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAg                                                                        >  1:2184386/1‑101 (MQ=255)
                              caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAg                                                                        >  2:1056063/1‑101 (MQ=255)
                              caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAg                                                                        <  1:4240796/101‑1 (MQ=255)
                                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCg                                                                     >  2:2829198/1‑101 (MQ=255)
                                 cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCg                                                                     <  2:3550826/101‑1 (MQ=255)
                                       gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                               >  2:4133052/1‑101 (MQ=255)
                                       gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                               >  2:465538/1‑101 (MQ=255)
                                        gttGTCACTTTCGCGGGGCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTccccc                                                              <  2:874945/100‑1 (MQ=255)
                                        aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTccccc                                                              <  1:1519990/101‑1 (MQ=255)
                                           gTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgc                                                           >  1:1189058/1‑101 (MQ=255)
                                              ccTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggg                                                        <  1:1624763/100‑1 (MQ=255)
                                               cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                       >  2:2725037/1‑101 (MQ=255)
                                                tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                      >  2:3945711/1‑101 (MQ=255)
                                                tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggggg                                                      >  1:3239532/1‑101 (MQ=255)
                                                   cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                   <  1:3457047/101‑1 (MQ=255)
                                                   cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGt                                                   >  1:2213866/1‑101 (MQ=255)
                                                     cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                 >  1:1708800/1‑101 (MQ=255)
                                                     cccgatcgaGCGTCGCCCCCCCCGCGGGGGCGGGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  1:4430061/93‑1 (MQ=38)
                                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  1:3742416/1‑101 (MQ=255)
                                                        ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  2:2606483/1‑101 (MQ=255)
                                                         ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:2866016/96‑1 (MQ=2)
                                                         ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:362189/96‑1 (MQ=2)
                                                           gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCTCCCCGCGGGGGCGTGGATCGaa                                           >  1:2702553/1‑101 (MQ=255)
                                                            ctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  1:4388192/99‑1 (MQ=2)
                                                            aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  1:3595838/1‑101 (MQ=255)
                                                             tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  2:3025890/2‑101 (MQ=2)
                                                             aGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         >  2:3471186/1‑101 (MQ=255)
                                                              gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCGGCAGTGTCA.GGTTTTCCA.GCGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         >  2:171963/1‑101 (MQ=2)
                                                              gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG..GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         <  2:1202685/101‑1 (MQ=2)
                                                              ccggccccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                        <  2:1983125/93‑1 (MQ=255)
                                                               cgccGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                       <  1:3299792/98‑1 (MQ=255)
                                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                       >  2:489493/1‑101 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                      >  2:1738643/1‑101 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                      >  1:4412703/1‑101 (MQ=255)
                                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACTgg                                      >  1:2042608/1‑101 (MQ=255)
                                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                      <  1:190855/101‑1 (MQ=255)
                                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                     >  2:1572560/1‑101 (MQ=255)
                                                                    ccccccccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                  <  2:2462964/99‑1 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                 >  2:1477728/1‑101 (MQ=255)
                                                                       cccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGAt                               >  1:3163244/1‑101 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                              >  1:373867/1‑101 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                              <  1:2749405/101‑1 (MQ=255)
                                                                         cccGGGGGGGCGGGGATCGAAACATCTGCGGGTAGTCGGCG..G.GCAGTGGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCt                             <  2:1140907/101‑1 (MQ=37)
                                                                           cgggggggGGTGGATCGAAACATCTGCATGTAGTCGGGG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTgg                           <  2:3200289/98‑1 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACATGTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                          >  2:4599516/1‑101 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                          >  1:475419/1‑101 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGGTAGGGATCTGgc                          >  2:374939/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGGTATGGATCTGgcgc                        >  1:908596/1‑101 (MQ=255)
                                                                                 ggCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                     >  2:2667554/1‑101 (MQ=255)
                                                                                  gCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                    >  1:4164529/1‑101 (MQ=255)
                                                                                  gCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                    >  1:2491245/1‑101 (MQ=255)
                                                                                   cGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCTCAAACg                   >  1:761529/1‑101 (MQ=255)
                                                                                   cGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                   >  1:4098536/1‑101 (MQ=255)
                                                                                   cGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                   >  2:2753365/1‑101 (MQ=255)
                                                                                    gTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                  >  2:4544386/1‑101 (MQ=255)
                                                                                     tGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                 >  2:3711471/1‑101 (MQ=255)
                                                                                      ggATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaa                >  2:4207078/1‑101 (MQ=255)
                                                                                       gATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa               <  2:389278/101‑1 (MQ=255)
                                                                                       gATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGaaa               >  1:196784/1‑101 (MQ=255)
                                                                                         tCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGt             >  1:259671/1‑101 (MQ=255)
                                                                                          gatctcATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc            <  2:2410554/96‑1 (MQ=255)
                                                                                          cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc            <  1:2270786/101‑1 (MQ=255)
                                                                                          cGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACTGGTATGGATCTGGCGCAAACGGAAAGTc            >  1:4044225/1‑101 (MQ=255)
                                                                                           gAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt           <  1:1056151/101‑1 (MQ=255)
                                                                                               cATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTcagatc       >  1:2410716/1‑96 (MQ=255)
                                                                                                ctCTGGATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt      <  2:761454/100‑1 (MQ=255)
                                                                                                 tCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc     <  2:4617803/101‑1 (MQ=255)
                                                                                                  cTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGtcgtcg    >  1:3119137/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG..G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCT  >  NC_014034/1395569‑1395769

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG