Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,763 C→G 14.2% intergenic (+575/‑356) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7630C→G14.2% 98.0 ‑2.0 ‑0.1297intergenic (+575/‑356)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (25/17):  ref base (117/137):  total (143/154)
Fisher's exact test strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.51e-01

Only 100 of 322 total aligned reads displayed.

CATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395664‑1395863
                                                                                                          |                                                                                                       
cATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                                                        >  2:4617126/1‑101 (MQ=255)
 gtCTGCATGTAGGCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                                       <  1:240478/100‑1 (MQ=255)
 aTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                                                       <  2:2213704/101‑1 (MQ=255)
  tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                                                      >  2:1550351/1‑101 (MQ=255)
   cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                                     >  1:3119137/1‑101 (MQ=255)
   cTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.tcgtcg                                                                                                     <  2:4412411/101‑1 (MQ=255)
    tGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGc                                                                                                    <  2:1708689/101‑1 (MQ=255)
     gCATGTAGTCGGGGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCCCt                                                                                                   <  1:2665783/101‑1 (MQ=255)
        tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                                                                >  1:3608255/1‑101 (MQ=255)
         gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                                               >  1:4197997/1‑101 (MQ=255)
          gaGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccccc                                                                                              <  2:541183/100‑1 (MQ=255)
           aGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                                                             <  1:4613562/101‑1 (MQ=255)
                 ggggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgggg                                                                                       <  1:1489895/100‑1 (MQ=255)
                   ggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGc                                                                                     >  2:4349142/1‑101 (MQ=255)
                    gCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCg                                                                                    >  2:4591591/1‑101 (MQ=255)
                    gCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCg                                                                                    <  2:1530540/101‑1 (MQ=255)
                        ttCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                                >  2:686877/1‑101 (MQ=255)
                          caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                                                                 <  2:3194019/94‑1 (MQ=2)
                          cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              <  1:4353550/101‑1 (MQ=255)
                           gAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             >  1:1950425/1‑101 (MQ=255)
                            ggCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                            <  2:2121320/100‑1 (MQ=255)
                            caaggctgTCGGTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                              >  2:2911267/8‑101 (MQ=2)
                              ccGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          <  2:3475789/101‑1 (MQ=255)
                              ccGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGGCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          <  2:1446838/101‑1 (MQ=255)
                              attcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                             <  1:3400467/98‑1 (MQ=2)
                               gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                           >  2:2798113/5‑101 (MQ=2)
                                gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                        <  1:4137029/101‑1 (MQ=255)
                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          <  1:2800545/101‑1 (MQ=2)
                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                          >  1:4535998/1‑101 (MQ=2)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                        <  1:1767462/100‑1 (MQ=2)
                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGGTCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCTg                                                                      >  2:2275947/1‑101 (MQ=255)
                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                                      <  1:489541/101‑1 (MQ=255)
                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                                                      <  1:943617/101‑1 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                                        <  2:1076338/101‑2 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                     >  1:2069500/1‑101 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.A.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                     <  1:3762313/101‑1 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                                                    <  1:4046988/101‑1 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                                      >  1:3511552/1‑98 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacc                                                                      >  1:2890673/1‑98 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                     >  2:3713949/1‑99 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                                                     >  1:535910/1‑97 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgcatg                                                                    <  2:3949448/101‑6 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gc                                                                 <  2:2184236/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gc                                                                 >  2:760698/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gc                                                                 <  1:814475/101‑1 (MQ=255)
                                       ccccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                                    <  2:3645967/99‑6 (MQ=0)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                                    >  1:1627564/1‑96 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                                   >  1:319939/1‑97 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                                   >  1:732437/1‑95 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT.GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                                  <  1:2952943/101‑6 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                  >  2:2698902/1‑94 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                  <  1:4104686/101‑8 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                              >  2:820515/1‑101 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACTCGGCAAGGACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                                               >  2:1878752/1‑91 (MQ=2)
                                              cgcgGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTg                                                         <  1:1550456/101‑1 (MQ=255)
                                                  ggggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAg                                                     >  2:2027370/1‑101 (MQ=255)
                                                   gggCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGt                                                    <  1:4313592/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGa                                                  <  2:4151534/101‑1 (MQ=255)
                                                     gCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGa                                                  <  1:2294245/101‑1 (MQ=255)
                                                       gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                <  2:3599411/101‑1 (MQ=255)
                                                       gTGGATCGAAACT.GGTATGGATCTG..GCGCAAACCT..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                <  1:4005472/101‑1 (MQ=255)
                                                        tGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAg                                               <  1:3637030/101‑1 (MQ=255)
                                                         ggATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGa                                              >  2:330087/1‑101 (MQ=255)
                                                         ggATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGa                                              <  1:4449181/101‑1 (MQ=255)
                                                          gATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.c                                            <  2:4092522/101‑1 (MQ=255)
                                                            tCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGc                                          <  1:2735402/101‑1 (MQ=255)
                                                              gAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCg                                        <  2:3118893/101‑1 (MQ=255)
                                                              gAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCg                                        <  2:2499347/101‑1 (MQ=255)
                                                               aaaCT.GGTATGGATCTG..TCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.AGCCgg                                       >  2:3756509/1‑100 (MQ=255)
                                                               aaaCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGt                                       >  2:4443462/1‑101 (MQ=255)
                                                                aaCT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTg                                      >  2:2548488/1‑101 (MQ=255)
                                                                  cT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                    <  1:1083238/101‑1 (MQ=255)
                                                                   t.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                  <  2:4258161/101‑1 (MQ=255)
                                                                   t.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                  <  1:1419298/101‑1 (MQ=255)
                                                                      gTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGt                                >  1:2493676/1‑101 (MQ=255)
                                                                      gTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGt                                >  2:1741887/1‑101 (MQ=255)
                                                                       tATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                               <  1:2851788/101‑1 (MQ=255)
                                                                         tGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGc                             >  2:35106/1‑101 (MQ=255)
                                                                          ggATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                            >  2:1117856/1‑101 (MQ=255)
                                                                           gATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTc                           <  1:2606668/101‑1 (MQ=255)
                                                                             tCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccc                         >  1:1197114/1‑101 (MQ=255)
                                                                              cTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccc                        <  1:1390594/101‑1 (MQ=255)
                                                                               tG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccc                       <  2:1269495/101‑1 (MQ=255)
                                                                                g..gCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                      <  1:3895310/101‑1 (MQ=255)
                                                                                   gcgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcg                     <  1:3441754/101‑1 (MQ=255)
                                                                                    cgcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgc                    <  2:4043940/101‑1 (MQ=255)
                                                                                     gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgcg                   <  1:3274760/101‑1 (MQ=255)
                                                                                     gcAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCcgcg                   <  2:2719661/101‑1 (MQ=255)
                                                                                       aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                 <  1:3972865/101‑1 (MQ=255)
                                                                                       aaaCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggg                 >  1:1911437/1‑101 (MQ=255)
                                                                                         aCGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCggggg               >  2:2452657/1‑101 (MQ=255)
                                                                                          cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc              <  1:1017993/101‑1 (MQ=255)
                                                                                          cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc              >  1:4638092/1‑101 (MQ=255)
                                                                                          cGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc              <  1:330119/101‑1 (MQ=255)
                                                                                            g..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCgg            >  2:1809591/1‑100 (MQ=255)
                                                                                               aaaGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg           <  2:1311376/101‑1 (MQ=255)
                                                                                                  gccccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTg           <  2:1999238/93‑1 (MQ=0)
                                                                                                   tCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc       <  1:2036305/101‑1 (MQ=255)
                                                                                                    cT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg      >  2:1169060/1‑101 (MQ=255)
                                                                                                          cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  >  1:1323786/1‑101 (MQ=255)
                                                                                                          |                                                                                                       
CATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATGGATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1395664‑1395863

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG