Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,884 G→C 20.9% intergenic (+696/‑235) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8840G→C20.9% 138.1 ‑0.4 0.0276intergenic (+696/‑235)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/26):  ref base (104/110):  total (137/139)
Fisher's exact test strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

GTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395784‑1395984
                                                                                                       |                                                                                                          
 tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.Cggc                                                                                                           >  2:2621326/1‑100 (MQ=255)
 tGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAGAGACGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...t                                                                                                        <  1:3978236/101‑1 (MQ=255)
   gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCg                                                                                                      <  2:3939035/101‑1 (MQ=255)
   gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCg                                                                                                      >  2:123555/1‑101 (MQ=255)
   gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCg                                                                                                      >  1:1856567/1‑101 (MQ=255)
       gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGCgcg                                                                                                  >  2:1463043/1‑101 (MQ=255)
        aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgcgc                                                                                                 <  1:2621511/101‑1 (MQ=255)
           ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGcgcc.gc                                                                                             <  2:3127792/101‑1 (MQ=255)
           ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGcgcc.gc                                                                                             <  1:192333/101‑1 (MQ=255)
            cTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCc.gcg                                                                                            >  1:2567840/1‑101 (MQ=255)
                    tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGcg                                                                                  >  1:3343160/1‑100 (MQ=255)
                    tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCt                                                                                  <  2:2040900/101‑1 (MQ=255)
                      aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcc                                                                                >  1:628910/1‑101 (MQ=255)
                          gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                                            >  1:4563616/1‑101 (MQ=255)
                             aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCcgcg                                                                         >  2:2191166/1‑101 (MQ=255)
                                   aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                   >  1:2730131/1‑101 (MQ=255)
                                   aGACGCCGGGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                   <  2:4364221/101‑1 (MQ=255)
                                    gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCgg                                                                  >  1:409313/1‑100 (MQ=255)
                                    gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                                  <  1:1110832/101‑1 (MQ=255)
                                     aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                                 <  2:4064493/101‑1 (MQ=255)
                                      gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                                <  2:1898996/91‑1 (MQ=9)
                                      gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                                >  2:2750397/11‑101 (MQ=9)
                                      ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCg                                                                   >  1:3759662/10‑101 (MQ=2)
                                       ccaacctatgTCGCTCTCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:1426767/92‑1 (MQ=9)
                                        ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCgcg                                                                 >  2:1789652/8‑99 (MQ=2)
                                         gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                >  2:2897103/7‑101 (MQ=2)
                                         cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             >  2:3355054/1‑101 (MQ=255)
                                         cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             >  1:1395841/1‑101 (MQ=255)
                                         aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:134388/94‑1 (MQ=9)
                                          gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:3580458/101‑1 (MQ=255)
                                           cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:3724910/97‑1 (MQ=2)
                                           cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:3637405/96‑1 (MQ=9)
                                           cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           >  2:247891/6‑101 (MQ=9)
                                           cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  2:467252/96‑1 (MQ=9)
                                           ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:2928945/97‑1 (MQ=2)
                                           ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  1:1510315/5‑101 (MQ=2)
                                            gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            >  2:4303417/4‑101 (MQ=2)
                                            ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  2:3705106/5‑101 (MQ=9)
                                            cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACG.CCGG.GGCTCTGGTCACGG...TTTAG.GAC.G.G..A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  2:3830265/97‑1 (MQ=2)
                                             tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCCCGGGGGGGTGGATCGaaa                                                         >  1:2770144/4‑101 (MQ=9)
                                             gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  1:217556/101‑1 (MQ=255)
                                             cccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:252443/99‑1 (MQ=2)
                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        >  2:2852548/3‑101 (MQ=9)
                                              atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        >  2:4607411/3‑101 (MQ=9)
                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  1:1757956/101‑1 (MQ=255)
                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  2:1378148/101‑1 (MQ=255)
                                               tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                       >  2:3056295/2‑100 (MQ=9)
                                               tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                       >  1:3867882/2‑100 (MQ=9)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:3197205/1‑101 (MQ=2)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                       <  2:2703636/101‑1 (MQ=255)
                                                gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...AGGGGC.CC.TCG.CACGTCGCTCCCCCCGCCGGGGCGTGGATCGGAAcaa                                                      >  2:742595/1‑99 (MQ=9)
                                                 ccGCTCCCCCCGCGGGGGCGTTGATCCAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                     <  2:4637791/100‑1 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGAC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                    <  2:2384799/101‑1 (MQ=255)
                                                  cGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                    >  2:1288220/1‑101 (MQ=9)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                   >  1:591223/1‑101 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                   >  2:3587832/1‑101 (MQ=255)
                                                   cccccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                   <  1:3355303/98‑1 (MQ=255)
                                                    cTCGCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                  <  2:695300/101‑1 (MQ=9)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                  >  1:499162/1‑101 (MQ=255)
                                                    cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                  >  1:161409/1‑101 (MQ=9)
                                                      ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                <  2:4392600/101‑1 (MQ=255)
                                                       cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                               <  1:123569/101‑1 (MQ=255)
                                                        ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                              >  2:2342545/1‑101 (MQ=255)
                                                        ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                              <  1:945405/101‑1 (MQ=255)
                                                          ccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGCGGTGGATCGAAACAATGCggcgcgg                                            >  2:2147493/1‑95 (MQ=2)
                                                          ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                               <  2:2935904/101‑10 (MQ=2)
                                                            gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                          >  2:3482348/1‑93 (MQ=2)
                                                             cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                         >  2:2065602/1‑92 (MQ=2)
                                                             cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                         <  2:1173885/101‑10 (MQ=2)
                                                             cgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTACCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggcc                                         >  1:4631869/1‑92 (MQ=2)
                                                             cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                         <  2:373830/101‑1 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                         >  1:3910554/1‑101 (MQ=255)
                                                              gggggCGTGTATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                        <  2:908516/101‑1 (MQ=255)
                                                              gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                        <  2:4236048/101‑1 (MQ=255)
                                                              gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCt                                        >  1:3831901/1‑101 (MQ=255)
                                                                gggCGTGTATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                      <  2:1927017/101‑1 (MQ=255)
                                                                gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                      >  1:2068326/1‑101 (MQ=255)
                                                                gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                      <  2:3321345/101‑1 (MQ=255)
                                                                 ggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                     >  1:3086284/1‑101 (MQ=255)
                                                                  gCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                    <  2:4100152/101‑1 (MQ=255)
                                                                   cGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                   >  1:1007820/1‑101 (MQ=255)
                                                                          cGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                            >  2:3245083/1‑101 (MQ=255)
                                                                           gAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                           >  1:3278182/1‑101 (MQ=255)
                                                                              aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                        <  2:2558792/101‑1 (MQ=255)
                                                                              aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                        <  1:133959/101‑1 (MQ=255)
                                                                              aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                        <  1:4349434/101‑1 (MQ=255)
                                                                                tGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                      <  1:3108390/101‑1 (MQ=255)
                                                                                       gc.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                <  1:727739/101‑1 (MQ=255)
                                                                                       gc.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                <  1:2258913/101‑1 (MQ=255)
                                                                                        c.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCCGTGGACTGGTCGCTc               >  2:449744/1‑101 (MQ=255)
                                                                                          cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc              <  1:2027510/101‑1 (MQ=255)
                                                                                           tATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc             <  1:35112/101‑1 (MQ=255)
                                                                                            aTCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc            >  1:2305418/1‑101 (MQ=255)
                                                                                            aTCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTcccc            >  2:1596439/1‑101 (MQ=255)
                                                                                                cATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc        >  1:1722311/1‑101 (MQ=255)
                                                                                                  tC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgg      >  1:145263/1‑101 (MQ=255)
                                                                                                   c.cGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg     <  1:4443757/101‑1 (MQ=255)
                                                                                                     cGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg    <  1:3158484/101‑1 (MQ=255)
                                                                                                     cGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg    >  1:121300/1‑101 (MQ=255)
                                                                                                       g...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc  <  1:2343820/101‑1 (MQ=255)
                                                                                                       |                                                                                                          
GTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395784‑1395984

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG