Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,886 C→G 19.2% intergenic (+698/‑233) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8860C→G19.2% 131.1 ‑0.1 ‑0.0280intergenic (+698/‑233)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (27/26):  ref base (111/113):  total (140/140)
Fisher's exact test strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.31e-01

Only 100 of 293 total aligned reads displayed.

GGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395786‑1395986
                                                                                                          |                                                                                                       
 gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCg                                                                                                        >  1:1856567/1‑101 (MQ=255)
      aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgggc                                                                                                   >  2:2440965/1‑101 (MQ=255)
      aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgcgc                                                                                                   >  1:270087/1‑101 (MQ=255)
         ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGcgcc.gc                                                                                               <  1:192333/101‑1 (MQ=255)
              ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.Gt                                                                                        >  1:3624288/1‑101 (MQ=255)
                cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                                      <  2:1197022/101‑1 (MQ=255)
                 gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGc                                                                                     >  1:2041478/1‑101 (MQ=255)
                      ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                                                                <  1:2301907/101‑1 (MQ=255)
                       tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                                                               <  1:3471444/101‑1 (MQ=255)
                        gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccccc                                                                              >  2:3205004/1‑101 (MQ=255)
                             tGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCCCTCCCCCCGCggg                                                                         >  2:3837015/1‑101 (MQ=255)
                              gATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCgggg                                                                        <  1:465586/101‑1 (MQ=255)
                                 aGACGCCGGGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                     <  2:4364221/101‑1 (MQ=255)
                                  gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCgg                                                                    >  1:409313/1‑100 (MQ=255)
                                  gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                                    <  1:1110832/101‑1 (MQ=255)
                                   aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:4064493/101‑1 (MQ=255)
                                    cGCCGTGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  2:2218044/101‑1 (MQ=255)
                                     ccaacctatgTCGCTCTCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:1426767/92‑1 (MQ=9)
                                      ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTg                                                                   >  1:3895311/8‑101 (MQ=2)
                                      ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:2611261/101‑1 (MQ=255)
                                      caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:3345442/93‑1 (MQ=9)
                                       cgtcgccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:2452823/95‑1 (MQ=255)
                                       cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  1:1395841/1‑101 (MQ=255)
                                        gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  2:2809621/101‑1 (MQ=255)
                                        gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:1252695/1‑101 (MQ=255)
                                        gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:2897279/1‑101 (MQ=255)
                                         tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:4438571/101‑1 (MQ=255)
                                         gggACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  1:686939/100‑1 (MQ=255)
                                         cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  2:2742181/5‑101 (MQ=2)
                                         cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCGCCC.GTCGCTCCCCCCCCGGGGGCGGGGaccca                                                             >  2:3070481/6‑97 (MQ=2)
                                         cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  2:467252/96‑1 (MQ=9)
                                         ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  1:1510315/5‑101 (MQ=2)
                                          gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:4303417/4‑101 (MQ=2)
                                          ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  2:3705106/5‑101 (MQ=9)
                                          cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACG.CCGG.GGCTCTGGTCACGG...TTTAG.GAC.G.G..A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  2:3830265/97‑1 (MQ=2)
                                          cggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  1:3799599/4‑101 (MQ=2)
                                           tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                           >  2:1353270/4‑101 (MQ=9)
                                           ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:1910619/3‑101 (MQ=2)
                                           ggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  1:4523734/3‑101 (MQ=2)
                                            gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  2:2152904/100‑1 (MQ=2)
                                            atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          >  2:4607411/3‑101 (MQ=9)
                                            aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          <  1:1757956/101‑1 (MQ=255)
                                             tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                         <  2:1484605/100‑2 (MQ=9)
                                              gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...AGGGGC.CC.TCG.CACGTCGCTCCCCCCGCCGGGGCGTGGATCGGAAcaa                                                        >  2:742595/1‑99 (MQ=9)
                                               tCGCTCCCCCCGCGGGGGGGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                       <  1:3837300/101‑1 (MQ=255)
                                               tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                       >  1:525646/1‑101 (MQ=9)
                                               ccGCTCCCCCCGCGGGGGCGTTGATCCAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                       <  2:4637791/100‑1 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                      >  2:3158240/1‑101 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATg                                                      >  1:3107067/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                     <  2:1304353/101‑1 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                     >  2:3587832/1‑101 (MQ=255)
                                                 gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                     >  1:2337891/1‑101 (MQ=9)
                                                 gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                     >  2:1945729/1‑101 (MQ=9)
                                                  cTCGCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                    <  2:695300/101‑1 (MQ=9)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                    >  1:2133240/1‑101 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                    >  2:2696962/1‑101 (MQ=255)
                                                  cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                    >  1:161409/1‑101 (MQ=9)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGG.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                  <  1:3975749/101‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                  <  2:4392600/101‑1 (MQ=255)
                                                     cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                 <  1:123569/101‑1 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                <  1:2236912/101‑1 (MQ=255)
                                                        ccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                                              <  1:3159004/101‑7 (MQ=2)
                                                        ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                              <  2:2348411/101‑1 (MQ=255)
                                                         cgcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                             >  2:2125526/1‑94 (MQ=2)
                                                         cgcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                             <  1:914419/101‑8 (MQ=2)
                                                         cgcgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                             >  2:2050680/1‑101 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                            >  2:3482348/1‑93 (MQ=2)
                                                           cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                           >  1:3670097/1‑101 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                           <  2:373830/101‑1 (MQ=255)
                                                            gggggCGTGTATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                          <  2:908516/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                          <  2:4236048/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                          >  1:3321594/1‑101 (MQ=255)
                                                            gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GCCGCTCCCCCCGCGGGGGCGCGGATCGAAACGATGCGCGCATGGCCt                                          >  2:3435101/1‑101 (MQ=38)
                                                             ggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTa                                         >  1:3753711/1‑101 (MQ=255)
                                                              gggCGTGGATCGAATCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                        <  2:3001182/101‑1 (MQ=255)
                                                                gCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                      <  2:2566770/101‑1 (MQ=255)
                                                                gCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                      <  2:4100152/101‑1 (MQ=255)
                                                                 cGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                     >  1:1007820/1‑101 (MQ=255)
                                                                         gAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                             <  1:4591890/101‑1 (MQ=255)
                                                                            aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                          <  1:133959/101‑1 (MQ=255)
                                                                            aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                          <  1:2879540/101‑1 (MQ=255)
                                                                            aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                          <  2:2490695/101‑1 (MQ=255)
                                                                            aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                          <  1:3578708/101‑1 (MQ=255)
                                                                              tGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                        >  2:2868243/1‑101 (MQ=255)
                                                                              tGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                        <  2:3282895/101‑1 (MQ=255)
                                                                               gcg.cCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                       >  1:2965408/1‑101 (MQ=255)
                                                                                cg.ccgc.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                      <  1:2548662/101‑1 (MQ=255)
                                                                                cg.ccgc.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                      <  1:2753563/101‑1 (MQ=255)
                                                                                      c.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCCGTGGACTGGTCGCTc                 >  2:449744/1‑101 (MQ=255)
                                                                                        cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                <  1:2027510/101‑1 (MQ=255)
                                                                                         tATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc               >  2:3348191/1‑101 (MQ=255)
                                                                                             gCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg           >  2:1711416/1‑101 (MQ=255)
                                                                                              cATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc          >  1:1722311/1‑101 (MQ=255)
                                                                                                tC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgg        >  1:145263/1‑101 (MQ=255)
                                                                                                 c.cGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg       >  1:620580/1‑101 (MQ=255)
                                                                                                   cGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg      >  1:121300/1‑101 (MQ=255)
                                                                                                     g...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc    <  1:3154479/101‑1 (MQ=255)
                                                                                                     g...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc    >  2:1813923/1‑101 (MQ=255)
                                                                                                         tCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCg   >  2:1120623/1‑101 (MQ=255)
                                                                                                          cGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAAGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt  <  2:1395746/101‑1 (MQ=255)
                                                                                                          |                                                                                                       
GGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395786‑1395986

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG