Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,887 G→T 9.3% intergenic (+699/‑232) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,8870G→T 9.3% 59.5 ‑0.9 ‑0.2280intergenic (+699/‑232)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/11):  ref base (124/130):  total (139/141)
Fisher's exact test strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.71e-01

Only 100 of 296 total aligned reads displayed.

GATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395787‑1395987
                                                                                                          |                                                                                                       
gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCg                                                                                                         >  1:1856567/1‑101 (MQ=255)
gATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCg                                                                                                         <  2:3939035/101‑1 (MQ=255)
  tCGAAACCTGCGGCGTAAGTTGCCAGTGATAGACGCCGCGGCCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAg                                                                                                       <  1:3116204/101‑1 (MQ=38)
     aaaCCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAgcgc                                                                                                    >  1:270087/1‑101 (MQ=255)
        ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGcgcc.gc                                                                                                <  1:192333/101‑1 (MQ=255)
        ccTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGcgcc.gc                                                                                                <  2:3127792/101‑1 (MQ=255)
               cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCg                                                                                       <  2:1197022/101‑1 (MQ=255)
                 tGAGTTGCCAGTGATAGACCCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCt                                                                                     <  2:2458898/101‑1 (MQ=255)
                   aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcc                                                                                   >  1:628910/1‑101 (MQ=255)
                     ttGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTcccc                                                                                 <  1:2301907/101‑1 (MQ=255)
                      tGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTccccc                                                                                <  1:3471444/101‑1 (MQ=255)
                                aGACGCCGGGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCg                                                                      <  2:4364221/101‑1 (MQ=255)
                                 gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGt                                                                     <  1:1110832/101‑1 (MQ=255)
                                  aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTg                                                                    <  2:4064493/101‑1 (MQ=255)
                                   gccaacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTgg                                                                   >  2:2750397/11‑101 (MQ=9)
                                   ccggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCg                                                                      >  1:3759662/10‑101 (MQ=2)
                                    ccaacctatgTCGCTCTCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  <  1:1426767/92‑1 (MQ=9)
                                     ccGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  1:2611261/101‑1 (MQ=255)
                                     caacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  1:3345442/93‑1 (MQ=9)
                                      gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                   >  2:2897103/7‑101 (MQ=2)
                                      cgtcgccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:2452823/95‑1 (MQ=255)
                                      cGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  1:1395841/1‑101 (MQ=255)
                                       gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:1162736/1‑101 (MQ=255)
                                       gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:1252695/1‑101 (MQ=255)
                                       acctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  1:4672341/95‑1 (MQ=9)
                                        tGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              <  1:4438571/101‑1 (MQ=255)
                                        cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:3724910/97‑1 (MQ=2)
                                        cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              <  1:3637405/96‑1 (MQ=9)
                                        cctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              <  2:467252/96‑1 (MQ=9)
                                        ccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 >  1:1510315/5‑101 (MQ=2)
                                         gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGTGCGCGCCAT.GAATT.C.A...TCGAGCGCCATCGAGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  2:4303417/4‑101 (MQ=2)
                                         ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             >  2:3705106/5‑101 (MQ=9)
                                         cgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACG.CCGG.GGCTCTGGTCACGG...TTTAG.GAC.G.G..A.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  2:3830265/97‑1 (MQ=2)
                                          tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                            >  2:1353270/4‑101 (MQ=9)
                                           atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           >  2:4607411/3‑101 (MQ=9)
                                           atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  2:3224078/99‑1 (MQ=9)
                                           atgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           >  2:2852548/3‑101 (MQ=9)
                                           aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                           <  1:1757956/101‑1 (MQ=255)
                                            tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                          <  2:1484605/100‑2 (MQ=9)
                                            tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                          >  1:3867882/2‑100 (MQ=9)
                                            tgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                          >  2:3056295/2‑100 (MQ=9)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                          >  1:4627881/1‑101 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                          <  2:2703636/101‑1 (MQ=255)
                                            cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                          <  2:2058949/101‑1 (MQ=255)
                                             gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...AGGGGC.CC.TCG.CACGTCGCTCCCCCCGCCGGGGCGTGGATCGGAAcaa                                                         >  2:742595/1‑99 (MQ=9)
                                              ccGCTCCCCCCGCGGGGGCGTTGATCCAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                        <  2:4637791/100‑1 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                      >  2:3587832/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                      >  1:591223/1‑101 (MQ=255)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                      <  2:1304353/101‑1 (MQ=255)
                                                gCTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGc                                                      >  1:2337891/1‑101 (MQ=9)
                                                 cTCGCCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                     <  2:695300/101‑1 (MQ=9)
                                                 cTCACCCCGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                     >  1:161409/1‑101 (MQ=9)
                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTGCT.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAGACGACGCGt                                                    >  2:2137605/1‑101 (MQ=37)
                                                   ccccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                   <  2:4392600/101‑1 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTc                                                  <  1:123569/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                 <  1:2236912/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                 <  1:945405/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                 <  2:1856441/101‑1 (MQ=255)
                                                      cccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                <  2:2493510/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgg                                               <  1:3159004/101‑7 (MQ=2)
                                                       ccGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGA...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                               >  2:2379463/1‑101 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACCCCG.CGGC.GTCCCGAAGA.CGGTCTTCTGGTGCC.GCT.GC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                                  <  2:2935904/101‑10 (MQ=2)
                                                         gcgGGGGCGTGGATCGAAACTGTG.CCGG.TTCCCGGAGA.CGC...TGGGGC.CC.TCG.CACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                             >  2:3482348/1‑93 (MQ=2)
                                                          cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                            >  1:3910554/1‑101 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                            <  2:373830/101‑1 (MQ=255)
                                                           gggggCGTGTATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                           <  2:908516/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                           <  2:4236048/101‑1 (MQ=255)
                                                           gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                           >  1:3321594/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCt                                           >  1:3831901/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GCCGCTCCCCCCGCGGGGGCGCGGATCGAAACGATGCGCGCATGGCCt                                           >  2:3435101/1‑101 (MQ=38)
                                                             gggCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                         >  1:2068326/1‑101 (MQ=255)
                                                               gCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                       <  2:4100152/101‑1 (MQ=255)
                                                               gCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                       <  2:1911302/101‑1 (MQ=255)
                                                                cGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                      >  1:1007820/1‑101 (MQ=255)
                                                                  tGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                    >  1:2941040/1‑101 (MQ=255)
                                                                         aaaCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGCAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                             >  1:4005113/1‑101 (MQ=255)
                                                                           aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                           <  1:133959/101‑1 (MQ=255)
                                                                           aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                           <  1:2879540/101‑1 (MQ=255)
                                                                           aCTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGAc                           <  1:3578708/101‑1 (MQ=255)
                                                                            cTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                          >  2:2294504/1‑101 (MQ=255)
                                                                               cg.ccgc.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                       <  1:2548662/101‑1 (MQ=255)
                                                                               cg.ccgc.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                       <  1:2753563/101‑1 (MQ=255)
                                                                                g.ccgc.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                      >  2:3190630/1‑101 (MQ=255)
                                                                                   cgc.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGc                    <  2:196760/101‑1 (MQ=255)
                                                                                    gc.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                   <  1:727739/101‑1 (MQ=255)
                                                                                     c.cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCCGTGGACTGGTCGCTc                  >  2:449744/1‑101 (MQ=255)
                                                                                       cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                 >  2:261341/1‑101 (MQ=255)
                                                                                       cTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcc                 <  1:2027510/101‑1 (MQ=255)
                                                                                        tATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                >  2:3348191/1‑101 (MQ=255)
                                                                                         aTCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc               >  1:4534352/1‑101 (MQ=255)
                                                                                            gCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg            >  2:1711416/1‑101 (MQ=255)
                                                                                             cATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcgc           >  1:1722311/1‑101 (MQ=255)
                                                                                               tC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgg         >  1:145263/1‑101 (MQ=255)
                                                                                                  cGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg       >  1:121300/1‑101 (MQ=255)
                                                                                                    g...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGc     >  2:2190412/1‑101 (MQ=255)
                                                                                                         cGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAAGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt   <  2:1395746/101‑1 (MQ=255)
                                                                                                          gAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGGGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg  >  1:4132243/1‑101 (MQ=255)
                                                                                                          gAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg  >  1:215473/1‑101 (MQ=255)
                                                                                                          gAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg  <  2:3286917/101‑1 (MQ=255)
                                                                                                          gAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTg  >  1:3106024/1‑101 (MQ=255)
                                                                                                          |                                                                                                       
GATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.CCGC.CTATCGCATC.CGG...TCGAGCGCC.GCG.CA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395787‑1395987

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG