Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,936 G→T 1.7% intergenic (+748/‑183) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,9360G→T 1.7% 2.2 ‑3.0 ‑0.2241intergenic (+748/‑183)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (126/111):  total (126/115)
Fisher's exact test strand distribution p-value = 5.04e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.74e-01

Only 100 of 273 total aligned reads displayed.

CTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCG  >  NC_014034/1395836‑1396035
                                                                                                     |                                                                                                   
cTCGCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                                                                     <  2:695300/101‑1 (MQ=9)
cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                                                                     <  2:267163/101‑1 (MQ=9)
 tCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGt                                                                                                    >  2:3742678/1‑101 (MQ=255)
  ccccccGCGGGGGCGTGGATCGAAACTGCGCCGGCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                   <  1:3975749/101‑1 (MQ=255)
  ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                                                                   >  1:1699364/1‑101 (MQ=255)
    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                 <  2:1856441/101‑1 (MQ=255)
    ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                                                                 <  1:945405/101‑1 (MQ=255)
     cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                                                                <  2:2493510/101‑1 (MQ=255)
      ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                               <  1:1354207/101‑1 (MQ=255)
      ccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATg                                                                                               <  2:2348411/101‑1 (MQ=255)
       cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGCTGGGGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                                                                              >  2:2125526/1‑94 (MQ=2)
       cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                              >  1:387395/1‑101 (MQ=255)
       cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                              <  2:1323693/101‑1 (MQ=255)
       cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                                                              >  2:2050680/1‑101 (MQ=255)
        gcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGc                                                                                             <  2:736191/101‑1 (MQ=255)
          gggggCGTGTATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCt                                                                                           <  2:908516/101‑1 (MQ=255)
          gggggCGTGGATCGAAACTGTGCCGGTTCCCGGAGCCGCT.GGGGACCTCGCACGTCGCTCCCCCAGCGGGGGCGTGGCTCGAAACAATGCggcgcgggcct                                                                                           >  2:1285368/1‑91 (MQ=2)
          gggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCCCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCt                                                                                           >  1:3831901/1‑101 (MQ=255)
            gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                                                                         <  2:3321345/101‑1 (MQ=255)
              gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                                                       <  2:2566770/101‑1 (MQ=255)
              gCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGt                                                                                       <  2:4100152/101‑1 (MQ=255)
               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                      >  1:1007820/1‑101 (MQ=255)
               cGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTc                                                                                      >  1:3337113/1‑101 (MQ=255)
                gTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                                                                     >  2:4381946/1‑101 (MQ=255)
                 tGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGGTCATGGCCTACGTCgg                                                                                    >  2:4322938/1‑101 (MQ=255)
                  ggATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                                                                   >  1:2034581/1‑101 (MQ=255)
                            tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                         <  1:3108390/101‑1 (MQ=255)
                            tGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                                                                         <  2:3282895/101‑1 (MQ=255)
                             gcgcCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                                                                        >  1:2965408/1‑101 (MQ=255)
                              cgccgcCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                       <  1:2548662/101‑1 (MQ=255)
                              cgccgcCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGt                                                                       >  1:2446792/1‑101 (MQ=255)
                               gccgccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                                                                      >  2:3190630/1‑101 (MQ=255)
                                ccgccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                                                                     <  2:4627580/101‑1 (MQ=255)
                                  gccTATCGCATCCGGTCGAGCGCCGCGCA.GGCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCt                                                                   <  1:2258913/101‑1 (MQ=255)
                                   ccTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTc                                                                  <  2:335098/101‑1 (MQ=255)
                                     tATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                                                                <  1:35112/101‑1 (MQ=255)
                                      aTCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccc                                                               >  1:4534352/1‑101 (MQ=255)
                                        cGCATCCGGTCGAGGGCCGCGCA.GTCGGTCCCCCCGCGGGGGCGGGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                                                             <  2:1223730/101‑1 (MQ=255)
                                         gCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                                                            >  1:3194702/1‑101 (MQ=255)
                                            tCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgg                                                         >  1:145263/1‑101 (MQ=255)
                                             ccGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg                                                        >  1:620580/1‑101 (MQ=255)
                                              cGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg                                                       <  1:2087001/101‑1 (MQ=255)
                                              cGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg                                                       >  1:121300/1‑101 (MQ=255)
                                               ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                      >  1:1470710/1‑101 (MQ=255)
                                               ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                      >  2:3587378/1‑101 (MQ=255)
                                               ggTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg                                                      >  1:1164052/1‑101 (MQ=255)
                                                  cGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                   >  2:3451097/1‑101 (MQ=255)
                                                  cGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAAGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGt                                                   <  2:1395746/101‑1 (MQ=255)
                                                      cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:1120712/101‑1 (MQ=255)
                                                       gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:2884114/101‑1 (MQ=255)
                                                       gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  1:4214816/1‑101 (MQ=255)
                                                       gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                              >  2:945959/1‑101 (MQ=255)
                                                       gaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  2:3654441/92‑1 (MQ=2)
                                                         acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:1558064/94‑1 (MQ=2)
                                                          gcgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  1:2374572/1‑101 (MQ=255)
                                                           cgcA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                          >  1:1740243/1‑101 (MQ=255)
                                                             gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTCGACCGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                         <  1:2705327/98‑1 (MQ=2)
                                                               aggcGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTc                                       <  2:2891010/98‑1 (MQ=255)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                    >  1:2615992/1‑101 (MQ=255)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATCGcc                                    >  1:743266/1‑101 (MQ=255)
                                                                  cGCTCCCCCAGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGcc                                    >  2:1509526/1‑101 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                                  >  1:2066366/1‑101 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                                  <  1:2297429/101‑1 (MQ=255)
                                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCt                                  <  2:4516982/101‑1 (MQ=255)
                                                                     tCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCACGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTc                                 <  1:3245328/101‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCg                                >  2:261006/1‑101 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCCCg                                >  2:2184752/1‑101 (MQ=255)
                                                                       cccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGa                               <  2:3910269/101‑1 (MQ=255)
                                                                       cccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGa                               >  2:2420221/1‑101 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGAt                              >  2:2840751/1‑101 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGCCCCCGAt                              >  2:193935/1‑101 (MQ=255)
                                                                         cccGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATc                             <  2:2729933/101‑1 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcg                           >  2:3103181/1‑101 (MQ=255)
                                                                            gcgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgc                          <  1:4116023/101‑1 (MQ=255)
                                                                             cgGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCcgcg                         >  1:1251371/1‑101 (MQ=255)
                                                                              gggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCgcgc                        >  1:1286013/1‑101 (MQ=255)
                                                                               ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                       <  1:2793985/101‑1 (MQ=255)
                                                                               ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                       >  1:3668144/1‑101 (MQ=255)
                                                                                gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                      <  2:499114/101‑1 (MQ=255)
                                                                                gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                      >  1:3462238/1‑101 (MQ=255)
                                                                                 ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                     <  2:1596489/101‑1 (MQ=255)
                                                                                   cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                   >  1:1802289/1‑101 (MQ=255)
                                                                                   cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCCCGATCCGCGCTTGcc                   >  2:2909397/1‑101 (MQ=255)
                                                                                     tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                 >  2:2005579/1‑101 (MQ=255)
                                                                                     tGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTc                 >  2:2277554/1‑101 (MQ=255)
                                                                                      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTcc                >  1:3158860/1‑100 (MQ=255)
                                                                                      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                >  2:4229782/1‑101 (MQ=255)
                                                                                      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                >  1:215771/1‑101 (MQ=255)
                                                                                      ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCa                <  1:858191/101‑1 (MQ=255)
                                                                                        aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg              >  2:3753561/1‑101 (MQ=255)
                                                                                        aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg              <  1:4382238/101‑1 (MQ=255)
                                                                                        aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACg              <  1:3718506/101‑1 (MQ=255)
                                                                                         tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGc             <  1:2015402/101‑1 (MQ=255)
                                                                                          cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGcc            >  2:1042557/1‑101 (MQ=255)
                                                                                            aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTg          >  2:4469514/1‑101 (MQ=255)
                                                                                             aaCGCTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc         <  2:2324272/101‑1 (MQ=255)
                                                                                             aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc         <  2:3003347/101‑1 (MQ=255)
                                                                                             aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc         >  2:2448755/1‑101 (MQ=255)
                                                                                             aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGc         >  1:4653511/1‑101 (MQ=255)
                                                                                                    cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCg  <  2:1699253/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
CTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTCACGCCTGCCGCGTCG  >  NC_014034/1395836‑1396035

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG