Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,014 T→C 12.7% intergenic (+826/‑105) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0140T→C12.7% 67.0 ‑0.4 ‑0.1236intergenic (+826/‑105)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/17):  ref base (98/108):  total (111/125)
Fisher's exact test strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.35e-01

Only 100 of 257 total aligned reads displayed.

GGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGG  >  NC_014034/1395914‑1396114
                                                                                                    |                                                                                                        
ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                                          >  1:1130848/1‑101 (MQ=255)
ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                                          >  2:3317456/1‑101 (MQ=255)
ggggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCt                                                                                                          >  1:3668144/1‑101 (MQ=255)
 gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                                                                         >  1:3462238/1‑101 (MQ=255)
 gggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCtt                                                                                                         <  2:499114/101‑1 (MQ=255)
  ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                                                                                        <  2:1596489/101‑1 (MQ=255)
  ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                                                                                        >  1:936474/1‑101 (MQ=255)
  ggCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTg                                                                                                        <  2:1470601/101‑1 (MQ=255)
    cGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGcc                                                                                                      >  1:4478421/1‑101 (MQ=255)
       ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.a                                                                                                  <  2:28980/101‑1 (MQ=255)
       ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.a                                                                                                  <  1:782550/101‑1 (MQ=255)
       ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.a                                                                                                  >  2:4229782/1‑101 (MQ=255)
       ggATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.a                                                                                                  <  1:858191/101‑1 (MQ=255)
           cGAAACGATGCGTTCACGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGcc                                                                                              >  1:1285202/1‑101 (MQ=255)
            gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCt                                                                                             <  2:2873276/101‑1 (MQ=255)
            gAAAAGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.GCGCCt                                                                                             <  1:3435355/101‑1 (MQ=255)
             aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                            >  1:2084398/1‑101 (MQ=255)
             aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                            >  2:2609507/1‑101 (MQ=255)
             aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACCCCtc                                                                                            >  1:4041580/1‑100 (MQ=255)
              aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                           >  1:4653511/1‑101 (MQ=255)
                   tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..Gt                                                                                   <  2:2335059/101‑1 (MQ=255)
                    gCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTc                                                                                  <  2:743191/101‑1 (MQ=255)
                     cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCg                                                                                 <  2:1699253/101‑1 (MQ=255)
                       ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCt                                                                               <  1:3205247/101‑1 (MQ=255)
                               cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                       <  1:4368012/101‑1 (MQ=255)
                                tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                      >  2:2652065/1‑101 (MQ=255)
                                 aCGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCgg                                                                     >  1:3145741/1‑101 (MQ=255)
                                  cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCggg                                                                    >  2:4074553/1‑101 (MQ=255)
                                    tCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCggggg                                                                  <  1:261373/101‑1 (MQ=255)
                                     cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                 <  1:4323230/101‑1 (MQ=255)
                                     cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                 <  2:3336864/101‑1 (MQ=255)
                                      gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                >  1:2970081/1‑101 (MQ=255)
                                      gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                <  2:3790043/101‑1 (MQ=255)
                                      gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                <  2:4041295/101‑1 (MQ=255)
                                       ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                               <  2:1007737/101‑1 (MQ=255)
                                         cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                             >  2:3148284/1‑101 (MQ=255)
                                         cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:215747/101‑1 (MQ=255)
                                           tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:2554513/94‑1 (MQ=2)
                                           gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  1:1588740/1‑101 (MQ=255)
                                            tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  1:2884199/1‑101 (MQ=255)
                                            tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:4469809/101‑1 (MQ=255)
                                             ggcgcggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:203776/96‑1 (MQ=9)
                                             ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:1696571/1‑101 (MQ=255)
                                              gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  2:1989406/97‑1 (MQ=9)
                                                gcggTCGCTTCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:4461188/3‑101 (MQ=2)
                                                gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       <  2:3920860/99‑1 (MQ=9)
                                                gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:935276/3‑101 (MQ=2)
                                                cgggTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  2:4534057/99‑1 (MQ=255)
                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:2259283/101‑1 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                    <  2:4136029/101‑2 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                   <  1:2840967/101‑1 (MQ=255)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                   >  1:1779890/1‑101 (MQ=255)
                                                   gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                   <  1:999350/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                  >  1:1243905/1‑98 (MQ=2)
                                                        tgcgcccGCGGGGGCGTGTATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTAGTGCGCGCTG..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                               <  2:1095472/97‑7 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                               <  2:1851575/101‑7 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                               >  1:4586988/1‑95 (MQ=2)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                              <  2:1262980/101‑1 (MQ=255)
                                                        tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                              >  2:3668696/1‑101 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                             >  1:3396542/1‑101 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                             <  2:1917427/101‑1 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                             <  2:591162/101‑1 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                             >  2:3520337/1‑93 (MQ=2)
                                                           ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                            <  2:1370798/101‑10 (MQ=2)
                                                               gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                       <  2:2084258/101‑1 (MQ=255)
                                                                cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGGGCATCTGAGGt                                      >  1:1338761/1‑101 (MQ=255)
                                                                 gggggCGTGGATGGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                     >  1:2531607/1‑101 (MQ=255)
                                                                  ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                    <  2:4478126/101‑1 (MQ=255)
                                                                  ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCGCATCTGATGtat                                    >  1:3761663/1‑101 (MQ=255)
                                                                   gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                   <  2:2305256/101‑1 (MQ=255)
                                                                     gCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATg                                 <  1:2191316/101‑1 (MQ=255)
                                                                       gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                               >  2:700290/1‑101 (MQ=255)
                                                                       gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                               >  2:254650/1‑101 (MQ=255)
                                                                       gTGGATCGAAACTCGCCCTCGATCCGCGCTGGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                               <  1:1509635/101‑1 (MQ=255)
                                                                        tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                              <  2:215449/101‑1 (MQ=255)
                                                                         ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                             <  1:1940198/101‑1 (MQ=255)
                                                                          gATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCgg                            >  2:4355629/1‑101 (MQ=255)
                                                                           aTCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCCGATGTATATGCCCGGt                           <  1:4074839/101‑1 (MQ=255)
                                                                              gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                        >  1:2499203/1‑101 (MQ=255)
                                                                              gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GCCGCTCCCCCCGCGGGGGCGTGGATCGCAACAGCCGCATCTGATGTATATGCCCGGTgag                        >  1:1494069/1‑101 (MQ=255)
                                                                                aaCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                      >  1:2741028/1‑101 (MQ=255)
                                                                                   tCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGc                   <  2:1163960/101‑1 (MQ=255)
                                                                                    cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                  <  2:1646758/101‑1 (MQ=255)
                                                                                    cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                  <  1:1814049/101‑1 (MQ=255)
                                                                                    cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                  <  1:3587646/101‑1 (MQ=255)
                                                                                      cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                >  2:3372681/1‑101 (MQ=255)
                                                                                      cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                <  1:1252788/101‑1 (MQ=255)
                                                                                       ccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc               <  1:3914908/101‑1 (MQ=255)
                                                                                        cTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCg              <  1:2277716/101‑1 (MQ=255)
                                                                                         tCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc             <  1:3317705/101‑1 (MQ=255)
                                                                                          cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt            >  2:3872460/1‑101 (MQ=255)
                                                                                          cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt            >  1:2161361/1‑101 (MQ=255)
                                                                                          cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt            >  1:2220908/1‑101 (MQ=255)
                                                                                          cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt            >  2:3069904/1‑101 (MQ=255)
                                                                                           gATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc           <  1:626277/101‑1 (MQ=255)
                                                                                              ccGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc        >  2:2749523/1‑101 (MQ=255)
                                                                                                gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc      <  2:2969852/101‑1 (MQ=255)
                                                                                                gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc      >  1:4090804/1‑101 (MQ=255)
                                                                                                  gcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc    <  1:1042640/101‑1 (MQ=255)
                                                                                                    ttGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgg  <  2:2514235/101‑1 (MQ=255)
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GGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGG  >  NC_014034/1395914‑1396114

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG