Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,023 G→T 13.1% intergenic (+835/‑96) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0230G→T13.1% 75.2 0.0 ‑0.2229intergenic (+835/‑96)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/17):  ref base (89/110):  total (102/127)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.10e-01

Only 100 of 246 total aligned reads displayed.

ATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395923‑1396123
                                                                                                     |                                                                                                       
aTCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACg                                                                                                         >  2:3753561/1‑101 (MQ=255)
 tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGc                                                                                                        <  2:2295264/101‑1 (MQ=255)
  cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGcc                                                                                                       >  2:254475/1‑101 (MQ=255)
  cGAAACGATGCGTTCACGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGcc                                                                                                       >  1:1285202/1‑101 (MQ=255)
   gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCt                                                                                                      <  2:2873276/101‑1 (MQ=255)
    aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                                     >  2:4469514/1‑101 (MQ=255)
    aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                                     >  1:2084398/1‑101 (MQ=255)
    aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACCCCtc                                                                                                     >  1:4041580/1‑100 (MQ=255)
     aaCGCTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                    <  2:2324272/101‑1 (MQ=255)
     aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                    >  1:4653511/1‑101 (MQ=255)
     aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                    >  2:2448755/1‑101 (MQ=255)
     aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                    <  2:3003347/101‑1 (MQ=255)
       cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.cg                                                                                                 <  2:2567666/101‑1 (MQ=255)
            cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCg                                                                                          <  2:1699253/101‑1 (MQ=255)
              ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCt                                                                                        <  1:3205247/101‑1 (MQ=255)
              ttCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCt                                                                                        >  1:2514409/1‑101 (MQ=255)
                     ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                 <  2:522928/101‑1 (MQ=255)
                      cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                                <  1:4368012/101‑1 (MQ=255)
                      cTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgc                                                                                >  1:4187512/1‑101 (MQ=255)
                       tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                               >  2:2652065/1‑101 (MQ=255)
                         cGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCggg                                                                             >  2:4074553/1‑101 (MQ=255)
                          gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                            >  2:875408/1‑101 (MQ=255)
                            cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                          <  1:4323230/101‑1 (MQ=255)
                            cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                          <  2:148951/101‑1 (MQ=255)
                              ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                        <  2:4563317/101‑1 (MQ=255)
                                cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  2:695263/1‑101 (MQ=255)
                                cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  2:3148284/1‑101 (MQ=255)
                                  tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:2554513/94‑1 (MQ=2)
                                  tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:2224401/94‑1 (MQ=2)
                                  tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:3425018/94‑1 (MQ=2)
                                   tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:4469809/101‑1 (MQ=255)
                                   tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:1905792/101‑1 (MQ=255)
                                   tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:2541175/101‑1 (MQ=255)
                                   tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   >  1:2884199/1‑101 (MQ=255)
                                    ggcgcggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:203776/96‑1 (MQ=9)
                                    ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:1696571/1‑101 (MQ=255)
                                     gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:1989406/97‑1 (MQ=9)
                                       gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  2:3920860/99‑1 (MQ=9)
                                       gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                <  1:2213099/99‑1 (MQ=9)
                                        tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:3553173/1‑101 (MQ=255)
                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                             >  2:3914623/1‑101 (MQ=255)
                                         ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                             <  2:121286/101‑1 (MQ=255)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                             <  2:4136029/101‑2 (MQ=2)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                            >  1:1779890/1‑101 (MQ=255)
                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                            <  1:2840967/101‑1 (MQ=255)
                                          gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                            <  1:999350/101‑1 (MQ=255)
                                          gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                            <  1:653094/101‑1 (MQ=255)
                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                           <  2:1031684/101‑1 (MQ=255)
                                           gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                           <  2:145249/100‑1 (MQ=255)
                                           gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                           <  2:2940811/100‑1 (MQ=255)
                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                           >  1:1243905/1‑98 (MQ=2)
                                              cTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGgcgc                                                        >  1:4082608/1‑101 (MQ=255)
                                               tgcgcccGCGGGGGCGTGTATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTAGTGCGCGCTG..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                        <  2:1095472/97‑7 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                        <  1:3504259/101‑7 (MQ=2)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCTCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                       <  1:2909626/101‑1 (MQ=255)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                       <  2:1262980/101‑1 (MQ=255)
                                               tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                       >  2:3668696/1‑101 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                      <  1:2190562/101‑1 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                      >  1:3396542/1‑101 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                      <  2:1917427/101‑1 (MQ=255)
                                                ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                      <  2:3277937/101‑1 (MQ=255)
                                                   cccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTg                                                   <  1:3046737/101‑1 (MQ=255)
                                                     cgcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAt                                                 <  1:37383/101‑1 (MQ=255)
                                                      gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                >  2:4370404/1‑101 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGGGCATCTGAGGt                                               >  1:1338761/1‑101 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                               >  1:4477014/1‑101 (MQ=255)
                                                       cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                               <  1:226760/101‑1 (MQ=255)
                                                         ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                             <  2:4478126/101‑1 (MQ=255)
                                                         ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                             >  1:1646869/1‑101 (MQ=255)
                                                         ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCGCATCTGATGtat                                             >  1:3761663/1‑101 (MQ=255)
                                                          gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                            <  1:261038/101‑1 (MQ=255)
                                                          gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                            <  1:4599816/101‑1 (MQ=255)
                                                          gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.AGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                            <  1:946039/101‑1 (MQ=255)
                                                           ggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTatat                                           <  1:2420385/101‑1 (MQ=255)
                                                             cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                         >  2:3342787/1‑101 (MQ=255)
                                                              gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                        >  2:721165/1‑101 (MQ=255)
                                                              gTGGATCGAAACTCGCCCTCGATCCGCGCTGGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                        <  1:1509635/101‑1 (MQ=255)
                                                               tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                       <  2:215449/101‑1 (MQ=255)
                                                                   tCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                   <  1:2005719/101‑1 (MQ=255)
                                                                     gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GCCGCTCCCCCCGCGGGGGCGTGGATCGCAACAGCCGCATCTGATGTATATGCCCGGTgag                                 >  1:1494069/1‑101 (MQ=255)
                                                                       aaCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGAc                               >  1:2741028/1‑101 (MQ=255)
                                                                           cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                           <  1:3587646/101‑1 (MQ=255)
                                                                           cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                           <  1:1814049/101‑1 (MQ=255)
                                                                           cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                           <  2:1646758/101‑1 (MQ=255)
                                                                             cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                         >  2:3372681/1‑101 (MQ=255)
                                                                             cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                         <  1:1252788/101‑1 (MQ=255)
                                                                              ccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                        <  1:3914908/101‑1 (MQ=255)
                                                                              ccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                        >  2:4349471/1‑101 (MQ=255)
                                                                                tCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc                      >  1:885535/1‑101 (MQ=255)
                                                                                 cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                     >  1:2161361/1‑101 (MQ=255)
                                                                                    tCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccc                  >  1:3286095/1‑101 (MQ=255)
                                                                                     ccGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                 >  2:2749523/1‑101 (MQ=255)
                                                                                       gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc               <  2:4055606/101‑1 (MQ=255)
                                                                                        cgcTTCCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg              <  2:2133090/101‑1 (MQ=255)
                                                                                         gcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc             <  1:1042640/101‑1 (MQ=255)
                                                                                             gCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg         <  2:936394/101‑1 (MQ=255)
                                                                                               cTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc       <  2:2615807/101‑1 (MQ=255)
                                                                                                 c.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGt     <  2:3831616/101‑1 (MQ=255)
                                                                                                   gcGCCTGC.AAC..GTCGCTCCACCCGCGGGGGCGTGGATCGAAACGGGCGCATCTGATGTATTTGCCGGGTGAGACGGCTGTCGCTCCCCCCGCGGGTGCGTg    <  1:405856/100‑1 (MQ=37)
                                                                                                    cGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTgg   <  1:721240/101‑1 (MQ=255)
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ATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395923‑1396123

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG