Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,024 C→A 13.1% intergenic (+836/‑95) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0240C→A13.1% 72.2 0.0 0.0228intergenic (+836/‑95)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/17):  ref base (87/111):  total (100/128)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

TCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395924‑1396124
                                                                                                     |                                                                                                       
tCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGc                                                                                                         <  2:2295264/101‑1 (MQ=255)
 cGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGcc                                                                                                        >  2:254475/1‑101 (MQ=255)
 cGAAACGATGCGTTCACGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGcc                                                                                                        >  1:1285202/1‑101 (MQ=255)
  gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCt                                                                                                       <  2:2873276/101‑1 (MQ=255)
   aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                                      <  1:3418462/101‑1 (MQ=255)
   aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                                      >  1:2084398/1‑101 (MQ=255)
    aaCGCTGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                     <  2:2324272/101‑1 (MQ=255)
    aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                     >  1:4653511/1‑101 (MQ=255)
    aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                     >  2:2448755/1‑101 (MQ=255)
    aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                     <  2:3003347/101‑1 (MQ=255)
      cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.cg                                                                                                  <  2:2567666/101‑1 (MQ=255)
           cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCg                                                                                           <  2:1699253/101‑1 (MQ=255)
                 tGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTcccc                                                                                     >  1:3320483/1‑101 (MQ=255)
                    ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                  <  2:522928/101‑1 (MQ=255)
                      tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                >  2:2652065/1‑101 (MQ=255)
                      tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                <  1:2550393/101‑1 (MQ=255)
                         gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                             >  2:875408/1‑101 (MQ=255)
                         gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCCC.ACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                             <  1:2868459/101‑1 (MQ=255)
                           cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                           <  2:148951/101‑1 (MQ=255)
                            gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                          <  2:3790043/101‑1 (MQ=255)
                            gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                          >  1:2970081/1‑101 (MQ=255)
                             ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                         <  2:4563317/101‑1 (MQ=255)
                             ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                         <  1:3451351/101‑1 (MQ=255)
                               cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                       >  2:695263/1‑101 (MQ=255)
                               cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                       >  2:3148284/1‑101 (MQ=255)
                               cAGTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                       <  1:3190873/101‑1 (MQ=255)
                                 tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                      <  1:2224401/94‑1 (MQ=2)
                                 gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     >  1:3576604/1‑101 (MQ=255)
                                  tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  2:1905792/101‑1 (MQ=255)
                                  tGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:4469809/101‑1 (MQ=255)
                                  ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     >  1:3210158/7‑101 (MQ=9)
                                  ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  2:4377724/95‑1 (MQ=9)
                                   ggcgcggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:203776/96‑1 (MQ=9)
                                   ggACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  1:1696571/1‑101 (MQ=255)
                                    gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  2:1989406/97‑1 (MQ=9)
                                      gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  1:2213099/99‑1 (MQ=9)
                                      cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                >  1:4055892/1‑101 (MQ=255)
                                      cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:2609692/101‑1 (MQ=255)
                                       tGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACTCGCCCTCCATCCACGCTTGCCTC.ACGCCTGC.CCC..GTCGCTCCACCCGCGGGGGCGTGGagctgaac                                                               >  2:405819/1‑94 (MQ=37)
                                       tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  2:3553173/1‑101 (MQ=255)
                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                              <  2:121286/101‑1 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                              >  2:4120081/1‑100 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                             >  1:1779890/1‑101 (MQ=255)
                                         gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                             <  1:999350/101‑1 (MQ=255)
                                         gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                             <  1:653094/101‑1 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                            <  2:1031684/101‑1 (MQ=255)
                                          gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                            <  2:2940811/100‑1 (MQ=255)
                                          gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                            <  2:145249/100‑1 (MQ=255)
                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                            >  1:1243905/1‑98 (MQ=2)
                                              tgcgcccGCGGGGGCGTGTATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTAGTGCGCGCTG..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                         <  2:1095472/97‑7 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                         >  1:2782159/1‑95 (MQ=2)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                        >  2:3668696/1‑101 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                        <  2:1262980/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatga                                                        <  1:4268064/101‑8 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  2:3277937/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  2:1917427/101‑1 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                       <  1:2190562/101‑1 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                      >  2:3971098/1‑92 (MQ=2)
                                                    cgcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGAt                                                  <  1:37383/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGGGCATCTGAGGt                                                >  1:1338761/1‑101 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                                >  1:4477014/1‑101 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                                <  1:226760/101‑1 (MQ=255)
                                                       gggggCGTGGATGGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                               >  1:2531607/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtat                                              >  1:1646869/1‑101 (MQ=255)
                                                        ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCGCATCTGATGtat                                              >  1:3761663/1‑101 (MQ=255)
                                                         gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                             <  1:4599816/101‑1 (MQ=255)
                                                         gggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.AGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGtata                                             <  1:946039/101‑1 (MQ=255)
                                                            cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                          >  2:3342787/1‑101 (MQ=255)
                                                             gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                         >  2:721165/1‑101 (MQ=255)
                                                             gTGGATCGAAACTCGCCCTCGATCCGCGCTGGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                         <  1:1509635/101‑1 (MQ=255)
                                                              tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                        <  2:215449/101‑1 (MQ=255)
                                                                gATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCgg                                      >  2:4355629/1‑101 (MQ=255)
                                                                 aTCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCCGATGTATATGCCCGGt                                     <  1:4074839/101‑1 (MQ=255)
                                                                  tCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTg                                    <  1:2005719/101‑1 (MQ=255)
                                                                    gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                  >  1:2499203/1‑101 (MQ=255)
                                                                    gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GCCGCTCCCCCCGCGGGGGCGTGGATCGCAACAGCCGCATCTGATGTATATGCCCGGTgag                                  >  1:1494069/1‑101 (MQ=255)
                                                                       aCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                               <  2:3919809/101‑1 (MQ=255)
                                                                          cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                            <  1:1814049/101‑1 (MQ=255)
                                                                          cGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCt                            <  2:1646758/101‑1 (MQ=255)
                                                                            cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                          >  2:3372681/1‑101 (MQ=255)
                                                                            cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                          <  1:1252788/101‑1 (MQ=255)
                                                                             ccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTc                         <  1:3914908/101‑1 (MQ=255)
                                                                               tCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc                       >  1:885535/1‑101 (MQ=255)
                                                                                cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGACACAGGCGCATCTCATGTATATGCCCGGTGGGACGGCtcgcgcc                      >  2:3870468/1‑95 (MQ=38)
                                                                                cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                      >  1:2161361/1‑101 (MQ=255)
                                                                                    ccGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                  >  2:2749523/1‑101 (MQ=255)
                                                                                     cgcgCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccccc                 >  1:4359143/1‑101 (MQ=255)
                                                                                      gcgcTTGCCTC.CCGCCTAC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                <  2:4187224/101‑1 (MQ=255)
                                                                                      gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                >  1:4090804/1‑101 (MQ=255)
                                                                                      gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                <  2:4476719/101‑1 (MQ=255)
                                                                                       cgcTTCCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcg               <  2:2133090/101‑1 (MQ=255)
                                                                                        gcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc              <  1:1042640/101‑1 (MQ=255)
                                                                                          ttGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgg            >  2:4507021/1‑101 (MQ=255)
                                                                                            gCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg          >  1:2901705/1‑101 (MQ=255)
                                                                                            gCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg          <  2:936394/101‑1 (MQ=255)
                                                                                              cTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        <  2:2615807/101‑1 (MQ=255)
                                                                                              cTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc        <  1:2697158/101‑1 (MQ=255)
                                                                                                   cGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTgg    <  1:721240/101‑1 (MQ=255)
                                                                                                     ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt  <  1:3963340/101‑1 (MQ=255)
                                                                                                     ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt  <  1:2342707/101‑1 (MQ=255)
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TCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1395924‑1396124

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG