Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,025 C→T 13.2% intergenic (+837/‑94) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,0250C→T13.2% 78.5 0.0 ‑0.0228intergenic (+837/‑94)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/17):  ref base (87/111):  total (100/128)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 245 total aligned reads displayed.

CGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395925‑1396125
                                                                                                     |                                                                                                       
cGAAACGATGCGTTCACGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGcc                                                                                                         >  1:1285202/1‑101 (MQ=255)
 gAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCt                                                                                                        <  2:2873276/101‑1 (MQ=255)
 gAAAAGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.GCGCCt                                                                                                        <  1:3435355/101‑1 (MQ=255)
  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                                       >  1:2084398/1‑101 (MQ=255)
  aaaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTg                                                                                                       <  1:3418462/101‑1 (MQ=255)
   aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                      >  1:4653511/1‑101 (MQ=255)
   aaCGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGc                                                                                                      >  2:2448755/1‑101 (MQ=255)
     cGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.cg                                                                                                   <  2:2567666/101‑1 (MQ=255)
        tGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..Gt                                                                                              <  2:2335059/101‑1 (MQ=255)
          cGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCg                                                                                            <  2:1699253/101‑1 (MQ=255)
                   ccTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcg                                                                                   <  2:522928/101‑1 (MQ=255)
                     tACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCcgcg                                                                                 >  2:2652065/1‑101 (MQ=255)
                        gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                              >  2:875408/1‑101 (MQ=255)
                        gTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCCC.ACGCCTGC.CGC..GTCGCTCCCCCCGCgggg                                                                              <  1:2868459/101‑1 (MQ=255)
                         tCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCggggg                                                                             <  1:261373/101‑1 (MQ=255)
                          cGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGc                                                                            <  2:148951/101‑1 (MQ=255)
                           gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                           >  1:2970081/1‑101 (MQ=255)
                           gggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCg                                                                           <  2:3790043/101‑1 (MQ=255)
                            ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                          <  2:1007737/101‑1 (MQ=255)
                            ggCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGt                                                                          <  2:4563317/101‑1 (MQ=255)
                              cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                        >  2:3148284/1‑101 (MQ=255)
                              cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                        >  2:695263/1‑101 (MQ=255)
                              cAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  2:215747/101‑1 (MQ=255)
                              cAGTGGACTGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                        <  1:3190873/101‑1 (MQ=255)
                                tctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  1:2224401/94‑1 (MQ=2)
                                gTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:3576604/1‑101 (MQ=255)
                                 ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      <  2:4377724/95‑1 (MQ=9)
                                 ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:3210158/7‑101 (MQ=9)
                                  ggcgcggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:203776/96‑1 (MQ=9)
                                   gcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:1989406/97‑1 (MQ=9)
                                     gcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:2213099/99‑1 (MQ=9)
                                     cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:2609692/101‑1 (MQ=255)
                                     cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:919845/101‑1 (MQ=255)
                                     cTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  1:4055892/1‑101 (MQ=255)
                                      tGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACTCGCCCTCCATCCACGCTTGCCTC.ACGCCTGC.CCC..GTCGCTCCACCCGCGGGGGCGTGGagctgaac                                                                >  2:405819/1‑94 (MQ=37)
                                      tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  1:1819565/1‑101 (MQ=255)
                                      tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  2:3553173/1‑101 (MQ=255)
                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  2:191010/1‑101 (MQ=9)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                               >  2:4120081/1‑100 (MQ=2)
                                        gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAg                                                              <  1:999350/101‑1 (MQ=255)
                                         gcGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgg                                                             <  2:2940811/100‑1 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtg                                                             >  1:1243905/1‑98 (MQ=2)
                                             tgcgcccGCGGGGGCGTGTATCGAAACTCGGCCTCGATCCG.GTCAGCCTCGTAGTGCGCGCTG..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                          <  2:1095472/97‑7 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                          <  2:1851575/101‑7 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                          >  1:2782159/1‑95 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatg                                                          >  1:4586988/1‑95 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                         <  2:1262980/101‑1 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCa                                                         >  2:3668696/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCAt                                                        <  1:2190562/101‑1 (MQ=255)
                                               cccccGGGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgac                                                        <  2:1642922/101‑9 (MQ=2)
                                                ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                       >  2:3971098/1‑92 (MQ=2)
                                                ccccGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCG.GTCAGCCTC..GTATTGCGCGCTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActtgatgacc                                                       <  2:1370798/101‑10 (MQ=2)
                                                    gcgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATg                                                  <  2:2084258/101‑1 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGGGCATCTGAGGt                                                 >  1:1338761/1‑101 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                                 >  1:4477014/1‑101 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGt                                                 <  1:226760/101‑1 (MQ=255)
                                                      gggggCGTGGATGGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGta                                                >  1:2531607/1‑101 (MQ=255)
                                                       ggggCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCCGCATCTGATGtat                                               >  1:3761663/1‑101 (MQ=255)
                                                           cGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGc                                           >  2:3342787/1‑101 (MQ=255)
                                                            gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                          >  2:721165/1‑101 (MQ=255)
                                                            gTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                          >  2:254650/1‑101 (MQ=255)
                                                            gTGGATCGAAACTCGCCCTCGATCCGCGCTGGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGcc                                          <  1:1509635/101‑1 (MQ=255)
                                                             tGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGccc                                         <  2:215449/101‑1 (MQ=255)
                                                              ggATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCg                                        >  2:3393876/1‑101 (MQ=255)
                                                               gATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCgg                                       >  2:4355629/1‑101 (MQ=255)
                                                                aTCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCCGATGTATATGCCCGGt                                      <  1:4074839/101‑1 (MQ=255)
                                                                   gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                   >  1:2499203/1‑101 (MQ=255)
                                                                   gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTgag                                   <  2:4214528/101‑1 (MQ=255)
                                                                   gAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GCCGCTCCCCCCGCGGGGGCGTGGATCGCAACAGCCGCATCTGATGTATATGCCCGGTgag                                   >  1:1494069/1‑101 (MQ=255)
                                                                      aCTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACg                                <  2:3919809/101‑1 (MQ=255)
                                                                       cTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACgg                               >  2:3330220/1‑101 (MQ=255)
                                                                        tCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGc                              <  2:1163960/101‑1 (MQ=255)
                                                                           cccTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGt                           <  1:1252788/101‑1 (MQ=255)
                                                                              tCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGc                        <  1:3317705/101‑1 (MQ=255)
                                                                               cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGACACAGGCGCATCTCATGTATATGCCCGGTGGGACGGCtcgcgcc                       >  2:3870468/1‑95 (MQ=38)
                                                                               cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                       >  1:802656/1‑101 (MQ=255)
                                                                               cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                       >  2:3069904/1‑101 (MQ=255)
                                                                               cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                       <  1:1809717/101‑1 (MQ=255)
                                                                               cGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCt                       >  1:2161361/1‑101 (MQ=255)
                                                                                gATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTc                      <  1:626277/101‑1 (MQ=255)
                                                                                   ccGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccc                   >  2:2749523/1‑101 (MQ=255)
                                                                                    cgcgCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTccccc                  >  1:4359143/1‑101 (MQ=255)
                                                                                     gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                 >  1:3865750/1‑101 (MQ=255)
                                                                                     gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                 <  2:4476719/101‑1 (MQ=255)
                                                                                     gcgcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTcccccc                 >  1:4090804/1‑101 (MQ=255)
                                                                                       gcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc               >  1:4393776/1‑101 (MQ=255)
                                                                                       gcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc               >  1:1696169/1‑101 (MQ=255)
                                                                                       gcTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCcgc               <  1:1042640/101‑1 (MQ=255)
                                                                                         ttGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgg             >  2:4507021/1‑101 (MQ=255)
                                                                                          tGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCggg            <  1:254499/101‑1 (MQ=255)
                                                                                           gCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg           >  1:2901705/1‑101 (MQ=255)
                                                                                           gCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg           <  1:1711527/101‑1 (MQ=255)
                                                                                           gCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCgggg           <  2:936394/101‑1 (MQ=255)
                                                                                             cTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGc         <  2:2615807/101‑1 (MQ=255)
                                                                                               c.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGt       <  1:4230070/101‑1 (MQ=255)
                                                                                                  cGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTgg     <  1:721240/101‑1 (MQ=255)
                                                                                                   gCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGa    <  1:3668971/101‑1 (MQ=255)
                                                                                                    ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt   <  1:3963340/101‑1 (MQ=255)
                                                                                                    ccTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt   <  1:2342707/101‑1 (MQ=255)
                                                                                                     cTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc  <  1:1596544/101‑1 (MQ=255)
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CGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGCCCTCGATCCGCGCTTGCCTC.ACGCCTGC.CGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395925‑1396125

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG