Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,808 G→A 13.4% V230V (GTG→GTA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8080G→A13.4% 88.1 ‑0.3 ‑0.1253V230V (GTG→GTARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/17):  ref base (100/119):  total (117/136)
Fisher's exact test strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.33e-01

Only 100 of 271 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGC  >  NC_014034/1396708‑1396907
                                                                                                     |                                                                                                      
cgCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCNTGGATCGAAACCGtg.tg                                                                                                        >  2:1116123/1‑101 (MQ=255)
 gCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.Tgg                                                                                                       >  2:2920995/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat                                                                                                     >  1:1494579/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat                                                                                                     >  2:2313506/1‑101 (MQ=255)
   cccccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat                                                                                                     <  2:1724878/100‑1 (MQ=255)
     cccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat...ga                                                                                                <  2:1220315/101‑1 (MQ=255)
        ccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCa                                                                                             >  2:3383131/1‑101 (MQ=255)
         cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCAt                                                                                            <  2:849397/101‑1 (MQ=255)
         cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCAt                                                                                            <  1:4323159/101‑1 (MQ=255)
         cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCAt                                                                                            <  1:3380478/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGc                                                                                          <  2:4645357/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGc                                                                                          >  1:403485/1‑101 (MQ=255)
           cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGc                                                                                          <  1:2288068/101‑1 (MQ=255)
            gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCa                                                                                         <  1:1276729/101‑1 (MQ=255)
            ggcggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCa                                                                                         <  1:3689551/98‑1 (MQ=255)
             ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAg                                                                                        >  2:3677034/1‑101 (MQ=255)
              gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgc                                                                                       <  1:1434167/101‑1 (MQ=255)
              gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgc                                                                                       <  2:3990575/101‑1 (MQ=255)
                gCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAgcgc                                                                                     <  1:225827/101‑1 (MQ=255)
                 cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCGTGCAGcgcg                                                                                    >  2:1325037/1‑101 (MQ=255)
                  gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCgg                                                                                   >  2:3001832/1‑101 (MQ=255)
                   tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGa                                                                                  >  2:2566180/1‑101 (MQ=255)
                   tGGATCGAAACCCTGTCCCTCAGATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGa                                                                                  <  1:2929290/101‑1 (MQ=255)
                    ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGAc                                                                                 >  2:2238351/1‑101 (MQ=255)
                      aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                               <  2:3913857/101‑1 (MQ=255)
                      aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACgg                                                                               >  1:3776978/1‑101 (MQ=255)
                       tCGAAGCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGc                                                                              <  1:1470657/101‑1 (MQ=255)
                        cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTG.TGGAT...GATCATGCAGCGCGGACGGCt                                                                             >  2:785573/1‑101 (MQ=255)
                        cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCt                                                                             >  1:2693095/1‑101 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                            >  1:1251995/1‑101 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                            >  1:2872257/1‑101 (MQ=255)
                         gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCtt                                                                            <  1:2313668/101‑1 (MQ=255)
                          aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCCGCGCGGACGGCTTg                                                                           >  2:499963/1‑101 (MQ=255)
                          aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                           >  1:2190075/1‑101 (MQ=255)
                          aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                           >  2:2071986/1‑101 (MQ=255)
                          aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTg                                                                           <  2:567777/101‑1 (MQ=255)
                             cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGt                                                                        <  2:3772227/101‑1 (MQ=255)
                              ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTc                                                                       <  1:2745194/101‑1 (MQ=255)
                               cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCg                                                                      >  2:4016181/1‑101 (MQ=255)
                                  tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTc                                                                   >  1:2611965/1‑101 (MQ=255)
                                      tCATATGCTGCCACGATGCCCTTGAGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                              <  2:3475171/101‑1 (MQ=255)
                                       cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                              >  2:3230932/1‑101 (MQ=255)
                                       cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                              <  1:3521962/101‑1 (MQ=255)
                                       cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTcccccc                                                              <  2:1483760/101‑1 (MQ=255)
                                           tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCgg                                                          <  1:1116212/101‑1 (MQ=255)
                                            gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggg                                                         <  2:421625/101‑1 (MQ=255)
                                              tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCggggg                                                       <  1:3083897/101‑1 (MQ=255)
                                              tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTG.CGGAT...GATCACGCAGCGCGGACGGCTTGGGGCGCGCCCCCCGCggggg                                                       >  1:1630647/1‑101 (MQ=37)
                                                 cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                    >  2:1993233/1‑100 (MQ=255)
                                                  aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                                   >  2:2856777/1‑101 (MQ=255)
                                                   cgctGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:909212/98‑1 (MQ=255)
                                                    gATGCCCTTGAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  2:1630542/101‑1 (MQ=255)
                                                    gATGCCCGTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGGGGa                                                 >  2:955767/1‑101 (MQ=255)
                                                     tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:636037/91‑1 (MQ=2)
                                                     aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  1:180723/1‑101 (MQ=255)
                                                     aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:2480959/101‑1 (MQ=255)
                                                      tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCTGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:396184/101‑1 (MQ=255)
                                                      cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:463661/92‑1 (MQ=0)
                                                       gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              >  1:805437/1‑101 (MQ=255)
                                                       acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:2989080/93‑1 (MQ=2)
                                                        acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTC..GACCGAG.TTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:4225396/94‑1 (MQ=2)
                                                         atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  1:1867608/95‑1 (MQ=2)
                                                          cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  1:3889691/101‑1 (MQ=255)
                                                           ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAAc                                          >  2:1812804/1‑101 (MQ=255)
                                                             gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                        >  2:4353544/1‑101 (MQ=255)
                                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                         >  1:1370549/2‑100 (MQ=2)
                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                         >  1:3475209/2‑100 (MQ=2)
                                                              aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGGCGCCTCCCCCGCGGGGGCGTGGATCCAAACCgg                                       >  2:2076235/1‑101 (MQ=37)
                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCGCGGTCGGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                        <  1:535317/101‑3 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                       <  2:4491560/101‑4 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGTCGTGGATCGAAAcatgg                                      >  1:1905184/1‑97 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                      <  1:4461483/101‑5 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                      >  1:24101/1‑97 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                    <  2:3440308/101‑1 (MQ=255)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                    >  2:1390651/1‑101 (MQ=255)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGcc                                    >  2:1523293/1‑101 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                     >  1:3813163/1‑96 (MQ=2)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                 >  2:3735663/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGGTGCGCTCGCGG.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                  <  2:1164565/101‑9 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                <  1:4105269/101‑1 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                <  1:1155781/101‑1 (MQ=255)
                                                                       ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                              <  2:2611780/101‑1 (MQ=255)
                                                                          cgcgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCCCCCCCCGCGGGGGCGGGGGTCGAAACCGGTCCAGTGATAGa                           >  1:633962/1‑101 (MQ=38)
                                                                           gcgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                          >  1:200110/1‑101 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                         >  2:1659363/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACCGTG.TGGAT...GATTATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                        >  1:4145516/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                        <  1:1018737/101‑1 (MQ=255)
                                                                                 gCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                    <  2:2507611/101‑1 (MQ=255)
                                                                                   gTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                  >  2:2270850/1‑101 (MQ=255)
                                                                                    tGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                 >  1:749560/1‑101 (MQ=255)
                                                                                     ggATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                <  2:4049799/101‑1 (MQ=255)
                                                                                       aTCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc              <  2:3209286/101‑1 (MQ=255)
                                                                                          gAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc           >  2:2675261/1‑101 (MQ=255)
                                                                                           aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa          >  1:2038048/1‑101 (MQ=255)
                                                                                           aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa          <  1:255593/101‑1 (MQ=255)
                                                                                           aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa          >  2:3078874/1‑101 (MQ=255)
                                                                                           aaaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa          <  2:3579321/101‑1 (MQ=255)
                                                                                            aaCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg         >  1:3222348/1‑101 (MQ=255)
                                                                                             aCCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa        <  1:863821/101‑1 (MQ=255)
                                                                                                gtg.tGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt     <  2:1300988/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGCAGCGCGGACGGCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGC  >  NC_014034/1396708‑1396907

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG