Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,813 A→G 13.4% D232G (GAT→GGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8130A→G13.4% 75.8 ‑0.2 ‑0.7248D232G (GAT→GGT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/17):  ref base (97/117):  total (114/134)
Fisher's exact test strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.79e-01

Only 100 of 271 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCC  >  NC_014034/1396713‑1396913
                                                                                                        |                                                                                                       
cccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGgat...ga                                                                                                         <  2:1220315/101‑1 (MQ=255)
  cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATc                                                                                                       >  1:1144959/1‑101 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCAt                                                                                                     <  2:849397/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCAt                                                                                                     <  1:4323159/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATg                                                                                                    <  1:3383381/101‑1 (MQ=255)
      cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGc                                                                                                   >  1:403485/1‑101 (MQ=255)
      cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGc                                                                                                   <  2:4645357/101‑1 (MQ=255)
       gggggCGTGTATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..a                                                                                                >  1:2277309/1‑101 (MQ=255)
        ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..Ag                                                                                               <  2:3458928/101‑1 (MQ=255)
          ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..Agcg                                                                                             >  2:3521704/1‑101 (MQ=255)
          ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCACGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..Agcg                                                                                             >  1:2356918/1‑101 (MQ=255)
            cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCGTGC..AGcgcg                                                                                           >  2:1325037/1‑101 (MQ=255)
             gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCgg                                                                                          >  2:3001832/1‑101 (MQ=255)
              tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGa                                                                                         >  1:1451926/1‑101 (MQ=255)
              tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGa                                                                                         >  1:2247754/1‑101 (MQ=255)
               ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGAc                                                                                        <  2:3222869/101‑1 (MQ=255)
               ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGAc                                                                                        <  1:2974115/101‑1 (MQ=255)
               ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGAc                                                                                        >  2:2238351/1‑101 (MQ=255)
                 aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACg.g                                                                                     >  1:3776978/1‑101 (MQ=255)
                   cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTG.TGGAT...GATCATGC..AGCGCGGACG.GCt                                                                                   >  2:785573/1‑101 (MQ=255)
                    gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                  <  1:899166/101‑1 (MQ=255)
                     aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..CGCGCGGACG.GCTTg                                                                                 >  2:499963/1‑101 (MQ=255)
                     aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                 <  2:567777/101‑1 (MQ=255)
                       aCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGt                                                                              >  1:3475429/1‑101 (MQ=255)
                        cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGt                                                                              >  2:2073594/1‑101 (MQ=255)
                              cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcc                                                                        <  2:4001571/101‑1 (MQ=255)
                                 ccATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccccc                                                                     <  2:3966676/100‑1 (MQ=255)
                                  cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccccc                                                                    <  1:1411448/101‑1 (MQ=255)
                                   atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                   <  1:2584655/101‑1 (MQ=255)
                                   atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                   <  1:1272622/101‑1 (MQ=255)
                                      tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCgg                                                                >  2:1585227/1‑101 (MQ=255)
                                       gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCggg                                                               <  2:421625/101‑1 (MQ=255)
                                         tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTG.CGGAT...GATCACGC..AGCGCGGACG.GCTTGGGGCGCGCCCCCCGCggggg                                                             >  1:1630647/1‑101 (MQ=37)
                                          gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGc                                                            <  1:1335289/101‑1 (MQ=255)
                                             aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                                         >  2:2856777/1‑101 (MQ=255)
                                               gATGCCCTTGAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:1630542/101‑1 (MQ=255)
                                               gATGCCCGTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGGGGa                                                       >  2:955767/1‑101 (MQ=255)
                                                tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:636037/91‑1 (MQ=2)
                                                aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:2003292/101‑1 (MQ=255)
                                                 tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:2277147/101‑1 (MQ=255)
                                                 cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:3011092/10‑101 (MQ=0)
                                                  gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:2556904/101‑1 (MQ=255)
                                                  gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:1917032/1‑101 (MQ=255)
                                                   acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGCTC..GACCGAG...TTCACGGCGG.GCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:4225396/94‑1 (MQ=2)
                                                    atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  1:2549706/7‑101 (MQ=2)
                                                     cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:284593/101‑1 (MQ=255)
                                                     cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:3889691/101‑1 (MQ=255)
                                                      ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                >  2:3716133/1‑101 (MQ=255)
                                                       ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTG.TAGTTCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:3931010/98‑1 (MQ=2)
                                                       gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:354658/98‑1 (MQ=2)
                                                        gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                              >  2:2045414/1‑101 (MQ=255)
                                                        gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                              >  2:4353544/1‑101 (MQ=255)
                                                        gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                              >  2:2112991/1‑101 (MQ=255)
                                                        gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                              >  1:1248166/1‑101 (MQ=255)
                                                        ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGAT....CTCAGTCTGGGTGCGCTCGCGGTTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                >  2:4031131/3‑101 (MQ=2)
                                                         cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                               >  2:3629301/2‑100 (MQ=2)
                                                         aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                             >  1:767946/1‑101 (MQ=255)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                              >  1:530319/1‑99 (MQ=2)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                              >  1:823039/1‑99 (MQ=2)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                             >  1:2911402/1‑98 (MQ=2)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                             <  2:4491560/101‑4 (MQ=2)
                                                           tCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAcatg                                             <  1:2456057/101‑4 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                            <  1:4461483/101‑5 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                          >  1:2304488/1‑101 (MQ=255)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                          >  2:1390651/1‑101 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                           >  1:3813163/1‑96 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                         <  2:3283615/101‑1 (MQ=255)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                         <  1:748881/101‑1 (MQ=255)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                          >  1:2684458/1‑95 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                        <  2:1164565/101‑9 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                      <  1:4105269/101‑1 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                      >  1:1713005/1‑101 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACGGCG.TAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                      <  1:199595/101‑11 (MQ=0)
                                                                  ccccGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                    <  2:2611780/101‑1 (MQ=255)
                                                                    ccGGGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                  <  2:3393740/101‑1 (MQ=255)
                                                                        gggggCGTGGATCGAAACCGTG.TGGAT...GATTATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                              >  1:4145516/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                              <  1:1018737/101‑1 (MQ=255)
                                                                        gggggCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCCGAGATAGAccc                              >  1:944597/1‑100 (MQ=255)
                                                                           ggCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                           <  1:2123608/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                          <  1:1480308/101‑1 (MQ=255)
                                                                               tGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                       <  2:1712894/101‑1 (MQ=255)
                                                                                ggATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                      <  1:1812930/101‑1 (MQ=255)
                                                                                 gATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                     >  1:2735566/1‑101 (MQ=255)
                                                                                  aTCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                    <  2:3209286/101‑1 (MQ=255)
                                                                                    cGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                  <  2:3777610/101‑1 (MQ=255)
                                                                                    cGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                  <  2:1451817/101‑1 (MQ=255)
                                                                                     gAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                 >  2:2675261/1‑101 (MQ=255)
                                                                                     gAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                 <  2:1484193/101‑1 (MQ=255)
                                                                                      aaaCCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                >  1:614368/1‑101 (MQ=255)
                                                                                      aaaCCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                <  2:1777652/101‑1 (MQ=255)
                                                                                       aaCCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg               >  1:3222348/1‑101 (MQ=255)
                                                                                         ccGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc             <  1:189207/101‑1 (MQ=255)
                                                                                          cGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg            >  2:2445451/1‑101 (MQ=255)
                                                                                          cGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg            <  2:3763606/101‑1 (MQ=255)
                                                                                           gtg.tGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt           <  2:1300988/101‑1 (MQ=255)
                                                                                                 gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc      >  1:3699024/1‑101 (MQ=255)
                                                                                                 gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc      <  2:4072002/101‑1 (MQ=255)
                                                                                                   t...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc    <  1:4628105/101‑1 (MQ=255)
                                                                                                   t...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc    <  2:2872041/101‑1 (MQ=255)
                                                                                                        atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccctc  >  1:1096611/1‑99 (MQ=255)
                                                                                                        |                                                                                                       
CCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTG.TGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCC  >  NC_014034/1396713‑1396913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG