Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,816 A→T 12.7% H233L (CAT→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8160A→T12.7% 92.7 ‑0.3 ‑0.0262H233L (CAT→CTT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/17):  ref base (100/127):  total (117/145)
Fisher's exact test strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.47e-01

Only 100 of 273 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCG  >  NC_014034/1396716‑1396915
                                                                                                       |                                                                                                      
ccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCa                                                                                                        >  2:3383131/1‑101 (MQ=255)
 cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                       <  2:849397/101‑1 (MQ=255)
 cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                       <  1:4323159/101‑1 (MQ=255)
 cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                       <  1:3380478/101‑1 (MQ=255)
 cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                       <  1:2905163/101‑1 (MQ=255)
   cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGc                                                                                                     >  1:403485/1‑101 (MQ=255)
    gggggCGTGTATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..a                                                                                                  >  1:2277309/1‑101 (MQ=255)
    ggcggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..a                                                                                                  <  1:3689551/98‑1 (MQ=255)
     ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..Ag                                                                                                 >  2:3677034/1‑101 (MQ=255)
       ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..Agcg                                                                                               >  2:3521704/1‑101 (MQ=255)
       ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCACGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..Agcg                                                                                               >  1:2356918/1‑101 (MQ=255)
           tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..GGGGCggg                                                                                           >  2:133146/1‑100 (MQ=255)
           tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGa                                                                                           >  1:2247754/1‑101 (MQ=255)
            ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGAc                                                                                          <  2:3222869/101‑1 (MQ=255)
               tCGAAGCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.Gc                                                                                      <  1:1470657/101‑1 (MQ=255)
               tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.Gc                                                                                      >  1:2699648/1‑101 (MQ=255)
                cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTGGAT...GATCATGC..AGCGCGGACG.GCt                                                                                     >  2:785573/1‑101 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                    <  1:899166/101‑1 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                    <  1:4507316/101‑1 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                    <  1:2313668/101‑1 (MQ=255)
                  aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..CGCGCGGACG.GCTTg                                                                                   >  2:499963/1‑101 (MQ=255)
                  aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                   <  2:567777/101‑1 (MQ=255)
                  aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                   >  2:2071986/1‑101 (MQ=255)
                          tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTc                                                                           <  2:2616594/101‑1 (MQ=255)
                           cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcc                                                                          <  2:4001571/101‑1 (MQ=255)
                            ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccc                                                                         >  1:2940287/1‑101 (MQ=255)
                            ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccc                                                                         >  2:1155689/1‑101 (MQ=255)
                               cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccccc                                                                      <  1:1411448/101‑1 (MQ=255)
                                atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                     <  1:2584655/101‑1 (MQ=255)
                                atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                     >  2:160747/1‑101 (MQ=255)
                                atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                     <  1:1272622/101‑1 (MQ=255)
                                 tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcgc                                                                    >  2:4627804/1‑101 (MQ=255)
                                    gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCggg                                                                 <  2:421625/101‑1 (MQ=255)
                                      tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTGCGGAT...GATCACGC..AGCGCGGACG.GCTTGGGGCGCGCCCCCCGCggggg                                                               >  1:1630647/1‑101 (MQ=37)
                                       gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGc                                                              <  1:1335289/101‑1 (MQ=255)
                                        ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCg                                                             <  1:379670/101‑1 (MQ=255)
                                         cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCgg                                                            >  2:1993233/1‑100 (MQ=255)
                                         cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGt                                                            <  2:2589778/101‑1 (MQ=255)
                                          aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTg                                                           >  2:2856777/1‑101 (MQ=255)
                                            gATGCCCGTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGGGGa                                                         >  2:955767/1‑101 (MQ=255)
                                             tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:636037/91‑1 (MQ=2)
                                              tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCTGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:396184/101‑1 (MQ=255)
                                              tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       <  2:2277147/101‑1 (MQ=255)
                                              cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  1:463661/92‑1 (MQ=0)
                                              cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  1:3011092/10‑101 (MQ=0)
                                                 atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  1:1867608/95‑1 (MQ=2)
                                                  cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  1:284593/101‑1 (MQ=255)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAAc                                                  >  2:1812804/1‑101 (MQ=255)
                                                    ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTAGTTCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  2:3931010/98‑1 (MQ=2)
                                                    gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   <  1:354658/98‑1 (MQ=2)
                                                     gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                >  1:1248166/1‑101 (MQ=255)
                                                     gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                >  2:4353544/1‑101 (MQ=255)
                                                     ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGAT....CTCAGTCTGGGTGCGCTCGCGGTTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                  >  2:4031131/3‑101 (MQ=2)
                                                      cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                 >  2:3629301/2‑100 (MQ=2)
                                                      cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                 >  1:3475209/2‑100 (MQ=2)
                                                      aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                               >  1:767946/1‑101 (MQ=255)
                                                      aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGGCGCCTCCCCCGCGGGGGCGTGGATCCAAACCgg                                               >  2:2076235/1‑101 (MQ=37)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                >  1:823039/1‑99 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCGCGGTCGGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                <  1:535317/101‑3 (MQ=2)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                               <  2:4491560/101‑4 (MQ=2)
                                                        tCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAcatg                                               <  1:2456057/101‑4 (MQ=2)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                            <  2:3440308/101‑1 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                           <  1:748881/101‑1 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                           <  2:3283615/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                          <  2:2932864/101‑9 (MQ=2)
                                                             ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                        <  1:4105269/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                        >  1:1713005/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                        <  2:1310431/101‑1 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                        <  1:199595/101‑11 (MQ=0)
                                                               ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                        <  1:3885/101‑11 (MQ=0)
                                                                 ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                    <  1:4248070/101‑1 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                 >  2:1659363/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                <  1:1018737/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                <  1:1131648/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCCGAGATAGAccc                                >  1:944597/1‑100 (MQ=255)
                                                                        ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                             <  1:2123608/101‑1 (MQ=255)
                                                                        ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                             <  1:3752958/101‑1 (MQ=255)
                                                                         gCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                            <  2:2507611/101‑1 (MQ=255)
                                                                         gCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                            <  1:1480308/101‑1 (MQ=255)
                                                                           gTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                          >  2:172390/1‑101 (MQ=255)
                                                                             ggATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                        <  2:4049799/101‑1 (MQ=255)
                                                                                 cGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                    <  2:3777610/101‑1 (MQ=255)
                                                                                  gacaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                   >  1:2667070/4‑101 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                   >  2:2675261/1‑101 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                   >  2:1500178/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                  >  1:614368/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                  >  1:2038048/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                  <  1:255593/101‑1 (MQ=255)
                                                                                    aaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                 <  2:1188644/101‑1 (MQ=255)
                                                                                    aaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                 >  1:3222348/1‑101 (MQ=255)
                                                                                      ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc               <  1:189207/101‑1 (MQ=255)
                                                                                      ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc               <  1:4178367/101‑1 (MQ=255)
                                                                                       cGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg              <  2:3763606/101‑1 (MQ=255)
                                                                                          gtgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg           >  2:3017081/1‑101 (MQ=255)
                                                                                             gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc        <  2:1433707/101‑1 (MQ=255)
                                                                                               t...gatCATGC..AGCGCGGACG.GCTGGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc      <  2:1469332/101‑1 (MQ=255)
                                                                                                   gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccc     >  2:2148416/1‑101 (MQ=255)
                                                                                                    atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccctc    >  1:1096611/1‑99 (MQ=255)
                                                                                                     tCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc   <  2:2304326/101‑1 (MQ=255)
                                                                                                     tCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc   <  2:3086578/101‑1 (MQ=255)
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CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCG  >  NC_014034/1396716‑1396915

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG