Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,817 T→G 13.3% H233Q (CAT→CTG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8170T→G13.3% 88.3 ‑0.3 ‑0.0257H233Q (CAT→CAGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/17):  ref base (100/122):  total (117/140)
Fisher's exact test strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.81e-01

Only 100 of 273 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCG  >  NC_014034/1396717‑1396917
                                                                                                       |                                                                                                       
cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                         <  2:849397/101‑1 (MQ=255)
cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                         <  1:4323159/101‑1 (MQ=255)
cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                         <  1:3380478/101‑1 (MQ=255)
cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                         <  1:2905163/101‑1 (MQ=255)
  cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGc                                                                                                       >  1:403485/1‑101 (MQ=255)
   gggggCGTGTATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..a                                                                                                    >  1:2277309/1‑101 (MQ=255)
   ggcggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..a                                                                                                    <  1:3689551/98‑1 (MQ=255)
    ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..Ag                                                                                                   >  2:3677034/1‑101 (MQ=255)
      ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..Agcg                                                                                                 >  2:3521704/1‑101 (MQ=255)
      ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCACGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..Agcg                                                                                                 >  1:2356918/1‑101 (MQ=255)
       gCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTAGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGGGGAT...GATCATGC..Agcac                                                                                                >  2:1152829/1‑99 (MQ=255)
        cGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCGTGC..AGcgcg                                                                                               >  2:1325037/1‑101 (MQ=255)
         gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCgg                                                                                              >  2:3001832/1‑101 (MQ=255)
          tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGa                                                                                             >  1:2247754/1‑101 (MQ=255)
          tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGa                                                                                             >  2:2566180/1‑101 (MQ=255)
           ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGAc                                                                                            <  2:1658855/101‑1 (MQ=255)
            gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACg                                                                                           >  2:2744996/1‑101 (MQ=255)
              tCGAAGCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.Gc                                                                                        <  1:1470657/101‑1 (MQ=255)
              tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.Gc                                                                                        >  1:2699648/1‑101 (MQ=255)
               cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTGGAT...GATCATGC..AGCGCGGACG.GCt                                                                                       >  2:785573/1‑101 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                      <  1:4507316/101‑1 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                      <  1:899166/101‑1 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCtt                                                                                      <  1:2313668/101‑1 (MQ=255)
                 aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..CGCGCGGACG.GCTTg                                                                                     >  2:499963/1‑101 (MQ=255)
                 aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                     <  2:567777/101‑1 (MQ=255)
                    cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGt                                                                                  >  2:2073594/1‑101 (MQ=255)
                          cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcc                                                                            <  2:4001571/101‑1 (MQ=255)
                           ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccc                                                                           >  1:2940287/1‑101 (MQ=255)
                              cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccccc                                                                        <  1:1411448/101‑1 (MQ=255)
                               atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccccccc                                                                       >  2:2647099/1‑100 (MQ=255)
                               atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                       <  1:2584655/101‑1 (MQ=255)
                               atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                       <  1:1272622/101‑1 (MQ=255)
                                tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcgc                                                                      >  2:4627804/1‑101 (MQ=255)
                                 atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcgcg                                                                     <  2:3237843/101‑1 (MQ=255)
                                  tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCgg                                                                    >  2:1585227/1‑101 (MQ=255)
                                   gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCggg                                                                   <  2:421625/101‑1 (MQ=255)
                                     tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTGCGGAT...GATCACGC..AGCGCGGACG.GCTTGGGGCGCGCCCCCCGCggggg                                                                 >  1:1630647/1‑101 (MQ=37)
                                      gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGc                                                                <  1:1335289/101‑1 (MQ=255)
                                       ccACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCg                                                               <  1:379670/101‑1 (MQ=255)
                                           gATGCCCGTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGGGGa                                                           >  2:955767/1‑101 (MQ=255)
                                            tcacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTgg                                                            <  2:636037/91‑1 (MQ=2)
                                             tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCTGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                         <  2:396184/101‑1 (MQ=255)
                                             cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                           <  1:463661/92‑1 (MQ=0)
                                             cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                           >  1:3011092/10‑101 (MQ=0)
                                              gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        >  2:1917032/1‑101 (MQ=255)
                                                atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                        <  1:1867608/95‑1 (MQ=2)
                                                 cTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  1:284593/101‑1 (MQ=255)
                                                   ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTAGTTCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  2:3931010/98‑1 (MQ=2)
                                                   gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                     <  1:354658/98‑1 (MQ=2)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                  >  2:2112991/1‑101 (MQ=255)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                  >  2:2045414/1‑101 (MQ=255)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                  >  2:4353544/1‑101 (MQ=255)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                  >  1:1248166/1‑101 (MQ=255)
                                                    ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGAT....CTCAGTCTGGGTGCGCTCGCGGTTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                    >  2:4031131/3‑101 (MQ=2)
                                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                   >  1:3475209/2‑100 (MQ=2)
                                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                   >  2:3629301/2‑100 (MQ=2)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCgg                                                 >  1:767946/1‑101 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                  >  1:823039/1‑99 (MQ=2)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCGCGGTCGGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                  <  1:535317/101‑3 (MQ=2)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                 <  2:4491560/101‑4 (MQ=2)
                                                       tCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                 <  1:2456057/101‑4 (MQ=2)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                              >  2:1390651/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                              <  2:3440308/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                             <  1:748881/101‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                            <  2:1164565/101‑9 (MQ=2)
                                                            ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                          >  1:1713005/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                          <  1:4105269/101‑1 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                          <  1:199595/101‑11 (MQ=0)
                                                              ccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctatgg                                          <  1:3885/101‑11 (MQ=0)
                                                              ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                        <  2:2611780/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                      <  1:4248070/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                  <  1:1018737/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                  <  1:1131648/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCCGAGATAGAccc                                  >  1:944597/1‑100 (MQ=255)
                                                                       ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                               <  1:3752958/101‑1 (MQ=255)
                                                                       ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                               <  1:2123608/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgcg                              <  1:1480308/101‑1 (MQ=255)
                                                                          gTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                            >  2:2270850/1‑101 (MQ=255)
                                                                           tGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                           <  2:1712894/101‑1 (MQ=255)
                                                                            ggATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                          <  2:4049799/101‑1 (MQ=255)
                                                                             gATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                         >  2:2296332/1‑101 (MQ=255)
                                                                                cGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                      <  2:1451817/101‑1 (MQ=255)
                                                                                cGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                      <  2:3777610/101‑1 (MQ=255)
                                                                                 gacaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                     >  1:2667070/4‑101 (MQ=255)
                                                                                 gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                     <  2:1484193/101‑1 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                    >  1:2038048/1‑101 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                    >  1:614368/1‑101 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                    <  1:255593/101‑1 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                    <  2:1777652/101‑1 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                    >  2:3078874/1‑101 (MQ=255)
                                                                                   aaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                   >  1:3222348/1‑101 (MQ=255)
                                                                                     ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                 <  1:189207/101‑1 (MQ=255)
                                                                                     ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                 <  1:4178367/101‑1 (MQ=255)
                                                                                      cGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                <  2:3763606/101‑1 (MQ=255)
                                                                                      cGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                >  2:2445451/1‑101 (MQ=255)
                                                                                       gtgtGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt               <  2:1300988/101‑1 (MQ=255)
                                                                                         gtgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg             <  2:3210238/101‑1 (MQ=255)
                                                                                              t...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc        <  2:2872041/101‑1 (MQ=255)
                                                                                                   atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccctc      >  1:1096611/1‑99 (MQ=255)
                                                                                                   atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc      >  2:338001/1‑101 (MQ=255)
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CGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCG  >  NC_014034/1396717‑1396917

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG