Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,827 A→G 14.3% T237A (ACG→GCG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8270A→G14.3% 75.7 ‑0.2 ‑1.2243T237A (ACG→GCG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/18):  ref base (94/112):  total (112/131)
Fisher's exact test strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.85e-02

Only 100 of 257 total aligned reads displayed.

TGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1396727‑1396927
                                                                                                         |                                                                                                     
tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGa                                                                                                       >  1:2247754/1‑101 (MQ=255)
tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGa                                                                                                       >  1:1451926/1‑101 (MQ=255)
 ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGAc                                                                                                      <  2:1658855/101‑1 (MQ=255)
 ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGAc                                                                                                      <  2:3222869/101‑1 (MQ=255)
  gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACg                                                                                                     >  2:2744996/1‑101 (MQ=255)
   aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACg.g                                                                                                   >  1:3776978/1‑101 (MQ=255)
    tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.Gc                                                                                                  >  1:2699648/1‑101 (MQ=255)
       aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                               >  2:4247782/1‑101 (MQ=255)
       aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTg                                                                                               >  2:588735/1‑101 (MQ=255)
          cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGt                                                                                            >  2:2073594/1‑101 (MQ=255)
           ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTc                                                                                           <  1:2745194/101‑1 (MQ=255)
             tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGc                                                                                         <  1:139998/101‑1 (MQ=255)
              gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCt                                                                                        >  2:4003438/1‑101 (MQ=255)
                 ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccc                                                                                     >  2:1155689/1‑101 (MQ=255)
                 ccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccc                                                                                     >  1:2940287/1‑101 (MQ=255)
                  cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccc                                                                                    >  1:4646107/1‑101 (MQ=255)
                   tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccccc                                                                                   >  1:2425587/1‑101 (MQ=255)
                    cATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTcccccc                                                                                  <  1:3521962/101‑1 (MQ=255)
                     atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTccccccc                                                                                 >  2:2647099/1‑100 (MQ=255)
                     atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                                 >  2:160747/1‑101 (MQ=255)
                     atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                                 <  1:1272622/101‑1 (MQ=255)
                     atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                                 <  1:2584655/101‑1 (MQ=255)
                     atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCcg                                                                                 >  2:3561534/1‑101 (MQ=255)
                         gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCggg                                                                             <  2:812241/101‑1 (MQ=255)
                         gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCggg                                                                             >  1:382258/1‑101 (MQ=255)
                          ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCgggg                                                                            >  1:3383951/1‑101 (MQ=255)
                           tgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGTGCGGAT...GATCACGC..AGCGCGGACG.GCTTGGGGCGCGCCCCCCGCggggg                                                                           >  1:1630647/1‑101 (MQ=37)
                                cgctGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  1:909212/98‑1 (MQ=255)
                                  aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  1:2003292/101‑1 (MQ=255)
                                  aTGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    >  1:180723/1‑101 (MQ=255)
                                   tGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCTGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:396184/101‑1 (MQ=255)
                                   cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:3011092/10‑101 (MQ=0)
                                    gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:805437/1‑101 (MQ=255)
                                    gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  2:2556904/101‑1 (MQ=255)
                                    gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  2:1917032/1‑101 (MQ=255)
                                    acatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:2871247/93‑1 (MQ=2)
                                      atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:1867608/95‑1 (MQ=2)
                                      atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  1:2549706/7‑101 (MQ=2)
                                         tGAGTCGCTCCCCTCGCGGGGGCGTGGATCGAACCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                             <  1:4007296/101‑1 (MQ=255)
                                         tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                             <  1:454440/101‑1 (MQ=255)
                                         ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGTGTAGTTCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               <  2:3931010/98‑1 (MQ=2)
                                          gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                            >  2:2045414/1‑101 (MQ=255)
                                          gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                            >  2:2112991/1‑101 (MQ=255)
                                          ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGAT....CTCAGTCTGGGTGCGCTCGCGGTTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                              >  2:4031131/3‑101 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                            >  2:3631583/1‑99 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                            >  1:823039/1‑99 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCGCGGTCGGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                            <  1:535317/101‑3 (MQ=2)
                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGt                                                          >  2:563352/1‑101 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                           >  1:2911402/1‑98 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGTCGTGGATCGAAAcatgg                                                          >  1:1905184/1‑97 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatgg                                                          <  1:4461483/101‑5 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTcc                                                        <  2:3440308/101‑1 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                       <  2:3283615/101‑1 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                                       <  1:1322040/101‑1 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                        <  1:3629569/101‑7 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGG..TGCGCTCGCG.G.TTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                                        <  1:733724/101‑7 (MQ=2)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                                     >  2:3735663/1‑101 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                                    <  2:1310431/101‑1 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCagag                                                    <  1:4105269/101‑1 (MQ=255)
                                                    ccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                                  <  1:2258836/101‑1 (MQ=255)
                                                     cccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATa                                                 <  1:1477626/101‑1 (MQ=255)
                                                      ccGGGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                                <  2:3393740/101‑1 (MQ=255)
                                                      ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                                <  1:4248070/101‑1 (MQ=255)
                                                        gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                                              <  2:44070/101‑1 (MQ=255)
                                                         cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                             <  1:2356412/101‑1 (MQ=255)
                                                          gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                            <  1:1018737/101‑1 (MQ=255)
                                                          gggggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                            <  1:1131648/101‑1 (MQ=255)
                                                             ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                                         <  1:2123608/101‑1 (MQ=255)
                                                             ggCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                                         <  1:3752958/101‑1 (MQ=255)
                                                               cGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                                       <  2:756386/101‑1 (MQ=255)
                                                                gTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                      >  2:2270850/1‑101 (MQ=255)
                                                                gTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                                      >  2:172390/1‑101 (MQ=255)
                                                                 tGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                                     >  1:749560/1‑101 (MQ=255)
                                                                   gATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                                   >  2:2296332/1‑101 (MQ=255)
                                                                    aTCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                                  >  1:4192734/1‑101 (MQ=255)
                                                                      cGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                                <  2:3777610/101‑1 (MQ=255)
                                                                       gacaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                               >  1:2667070/4‑101 (MQ=255)
                                                                       gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                               >  2:4598020/1‑101 (MQ=255)
                                                                       gAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                               >  2:1500178/1‑101 (MQ=255)
                                                                        aaaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                              <  2:1777652/101‑1 (MQ=255)
                                                                         aaCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                             >  1:3222348/1‑101 (MQ=255)
                                                                          aCCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                            <  2:4645806/101‑1 (MQ=255)
                                                                           ccGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGAc                           >  1:1188569/1‑101 (MQ=255)
                                                                            cGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACg                          >  2:2445451/1‑101 (MQ=255)
                                                                               gtgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                       <  2:1264005/101‑1 (MQ=255)
                                                                               gtgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg                       >  2:3017081/1‑101 (MQ=255)
                                                                                tgGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGc                      >  2:894744/1‑101 (MQ=255)
                                                                                  gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc                    <  2:1433707/101‑1 (MQ=255)
                                                                                  gat...gatCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTc                    <  2:4072002/101‑1 (MQ=255)
                                                                                    t...gatCATGC..AGCGCGGACG.GCTGGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc                  <  2:1469332/101‑1 (MQ=255)
                                                                                         atCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccctc                >  1:1096611/1‑99 (MQ=255)
                                                                                          tCATGG..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc               <  2:2610581/101‑1 (MQ=255)
                                                                                          tCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc               <  2:3086578/101‑1 (MQ=255)
                                                                                             tGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCcgcg            >  2:1006468/1‑101 (MQ=255)
                                                                                               c..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg          >  1:1540687/1‑101 (MQ=255)
                                                                                               c..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg          <  1:2196747/101‑1 (MQ=255)
                                                                                               c..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg          <  1:588796/101‑1 (MQ=255)
                                                                                               c..AGCGCGGACG.GCTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCggg          >  2:2620381/1‑101 (MQ=255)
                                                                                                     gcgGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCg      <  1:1993368/101‑1 (MQ=255)
                                                                                                       ggACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTg    >  2:2201751/1‑101 (MQ=255)
                                                                                                         |                                                                                                     
TGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGC..AGCGCGGACG.GCTTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1396727‑1396927

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG