Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,955 C→A 13.2% G279G (GGC→ATA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9550C→A13.2% 96.4 ‑0.9 0.0342G279G (GGC→GGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/18):  ref base (130/137):  total (173/169)
Fisher's exact test strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396855‑1397055
                                                                                                         |                                                                                                          
cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                                            <  2:1144867/101‑1 (MQ=255)
cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                                            <  2:3003358/101‑1 (MQ=255)
 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACA.GCCT.GCGCG.GG.CGCA.TCGGCt                                                                                                           >  2:1619760/1‑101 (MQ=255)
   ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.Tc                                                                                                        >  2:4250725/1‑101 (MQ=255)
     aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.Cg                                                                                                     <  1:1523402/101‑1 (MQ=255)
     aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.Cg                                                                                                     >  2:3148728/1‑101 (MQ=255)
        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                                 <  2:2383937/101‑1 (MQ=255)
        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                                 <  1:1094541/101‑1 (MQ=255)
        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                                 <  1:1872164/101‑1 (MQ=255)
        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                                 <  2:2247220/101‑1 (MQ=255)
          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                                             <  2:1532053/101‑1 (MQ=255)
          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                                             <  2:1890588/101‑1 (MQ=255)
            ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..Cgg                                                                                           >  2:1347213/1‑100 (MQ=255)
             cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..Cggg                                                                                          >  1:627415/1‑99 (MQ=255)
              ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGCg.g                                                                                        >  1:3224278/1‑101 (MQ=255)
               gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCGCGCGGGGGCGTGGATCGAAGCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CCG..CGGg.gc                                                                                       >  1:3985993/1‑100 (MQ=38)
               gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.Gt                                                                                       <  1:2045554/101‑1 (MQ=255)
                    gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTc                                                                                  <  1:527195/101‑1 (MQ=255)
                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccc                                                                               <  2:4145934/101‑1 (MQ=255)
                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                                             <  2:1248073/101‑1 (MQ=255)
                         aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCCcccccc                                                                             >  2:210502/1‑101 (MQ=255)
                          gtccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCACCCcg                                                                            <  2:944517/99‑1 (MQ=255)
                              gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                        <  2:3347745/101‑1 (MQ=255)
                              gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                        >  1:1477450/1‑101 (MQ=255)
                                gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                      <  1:2271012/101‑1 (MQ=255)
                                 cgGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                     <  1:2493101/101‑1 (MQ=255)
                                 cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                     <  1:1034042/101‑1 (MQ=255)
                                     cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:4598320/101‑1 (MQ=255)
                                      ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 >  1:1780407/11‑101 (MQ=9)
                                       gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  2:3607657/92‑1 (MQ=9)
                                        gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgggt                                                               >  2:4362149/8‑99 (MQ=0)
                                        gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               <  2:826403/94‑1 (MQ=2)
                                        ccccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:1919304/94‑1 (MQ=0)
                                         ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  2:3939412/8‑101 (MQ=2)
                                         ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:2797965/101‑1 (MQ=255)
                                         cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  2:1207063/7‑101 (MQ=2)
                                         caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTc                                                              >  1:4261340/8‑101 (MQ=9)
                                         caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  2:233688/94‑1 (MQ=9)
                                          aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:4392637/95‑1 (MQ=9)
                                           gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  1:3430769/97‑1 (MQ=2)
                                            ggtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGGGCG.TCTGGA.ACA..GGAT.GTCGCTCCCCCCGCCGGGGCGTGGAtggga                                                           >  1:3690788/5‑97 (MQ=0)
                                            cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  1:3730702/5‑101 (MQ=2)
                                            aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          >  2:1756737/1‑101 (MQ=255)
                                             tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGGGTGGATCGaaa                                                          >  2:1783993/3‑101 (MQ=2)
                                               ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCCCGGGGGCGGGGGTCGAAActa                                                       >  1:759243/2‑99 (MQ=0)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcc                                                        >  2:472197/1‑100 (MQ=2)
                                               cGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       <  2:563786/101‑1 (MQ=255)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         <  1:1240454/101‑1 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                                       >  2:4608724/1‑99 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCCCAACac                                                       >  2:3204231/1‑100 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        <  2:2825788/101‑1 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                     <  2:2444381/101‑1 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                       >  2:4065884/1‑100 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                       <  1:3073927/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                                    >  1:1653918/1‑101 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                      <  1:1346730/101‑3 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                    <  2:346236/101‑6 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                    >  2:2959887/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                   >  1:97918/1‑101 (MQ=255)
                                                    ccccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                                  <  2:4447132/99‑8 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                    <  2:3521576/101‑2 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                  >  1:2141991/1‑94 (MQ=9)
                                                     gccccccGCGGGGGCGTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                 <  1:210526/100‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                   >  1:2893108/1‑99 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGGGGGGGCGTGGATCGAAAcccgccacg                                                 >  1:3922382/1‑93 (MQ=0)
                                                      ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                <  2:2699672/101‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                <  1:2572266/101‑10 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                <  1:4485809/101‑10 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                 >  1:2991329/1‑93 (MQ=9)
                                                       cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                <  1:2700506/101‑10 (MQ=9)
                                                        ccccGCGGGGGCTTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTC..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                              <  2:200086/101‑1 (MQ=255)
                                                        ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                               >  1:421990/1‑91 (MQ=2)
                                                        ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCTGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                              >  1:855830/1‑101 (MQ=255)
                                                           cgggggggCGTGCATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                           <  2:1426817/98‑1 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                           >  2:2638554/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                           >  1:378779/1‑101 (MQ=255)
                                                             ggggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                         <  2:2189925/100‑1 (MQ=255)
                                                                    gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                                  >  1:2625043/1‑101 (MQ=255)
                                                                     tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                 >  2:3703090/1‑101 (MQ=255)
                                                                      ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCCGCCGGa                                >  2:1108903/1‑101 (MQ=255)
                                                                      ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                >  2:1046852/1‑101 (MQ=255)
                                                                      ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                <  2:3776703/101‑1 (MQ=255)
                                                                        aTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                              >  2:4014005/1‑101 (MQ=255)
                                                                          cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                            <  2:2494166/101‑1 (MQ=255)
                                                                             aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAATCATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                         >  1:4036891/1‑101 (MQ=255)
                                                                              aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                        <  1:3150124/101‑1 (MQ=255)
                                                                               cA.GCCG.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                       <  1:894820/101‑1 (MQ=255)
                                                                                a.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGt                      <  2:2037908/101‑1 (MQ=255)
                                                                                  gCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                     >  1:3512471/1‑101 (MQ=255)
                                                                                    cT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGGTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                   >  2:2538739/1‑101 (MQ=255)
                                                                                        cgcg.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc                >  2:1320461/1‑101 (MQ=255)
                                                                                        cgcg.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc                >  2:701828/1‑101 (MQ=255)
                                                                                             gC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc            <  2:4511398/101‑1 (MQ=255)
                                                                                                 gCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg         >  1:2411195/1‑101 (MQ=255)
                                                                                                 gCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg         >  1:2214320/1‑101 (MQ=255)
                                                                                                   a.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg       >  2:3046191/1‑101 (MQ=255)
                                                                                                   a.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg       >  1:2390105/1‑101 (MQ=255)
                                                                                                      cgccT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg     <  2:1688783/98‑1 (MQ=255)
                                                                                                        gCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg   >  1:1973543/1‑101 (MQ=255)
                                                                                                        gCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg   <  2:855754/101‑1 (MQ=255)
                                                                                                         |                                                                                                          
CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396855‑1397055

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG