BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,960	G→T	10.5%	R281L (CGC→TTT)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,960	0	G→T	10.5%	85.4 ‑0.7 ‑0.4	332	R281L (CGC→CTC)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (20/15): ref base (146/151): total (166/166)
Fisher's exact test strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.29e-01

Only 100 of 362 total aligned reads displayed.


ATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396860‑1397060


aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                                          <  1:1523402/101‑1 (MQ=255)


aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                                          >  2:3148728/1‑101 (MQ=255)


   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGTCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                      <  2:2383937/101‑1 (MQ=255)


   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                      <  1:1094541/101‑1 (MQ=255)


   gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                      <  2:2247220/101‑1 (MQ=255)


    aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTg                                                                                                     <  1:187150/101‑1 (MQ=255)


     aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                                  <  2:1532053/101‑1 (MQ=255)


     aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                                  <  2:1890588/101‑1 (MQ=255)


      aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..Cg                                                                                                 >  2:162702/1‑101 (MQ=255)


         ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg.g                                                                                             <  1:2691227/101‑1 (MQ=255)


         ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGCg.g                                                                                             >  1:3224278/1‑101 (MQ=255)


           tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                           <  1:2797972/101‑1 (MQ=255)


           tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                           <  2:463615/101‑1 (MQ=255)


            ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCg                                                                                          <  1:3079118/101‑1 (MQ=255)


             cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGc                                                                                         <  1:3716408/101‑1 (MQ=255)


                agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcc                                                                                      >  2:4007777/1‑101 (MQ=255)


                 gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                                     >  2:4221109/1‑101 (MQ=255)


                    aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCCcccccc                                                                                  >  2:210502/1‑101 (MQ=255)


                     gtccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCACCCcg                                                                                 <  2:944517/99‑1 (MQ=255)


                       ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCCCCCCCcgcg                                                                               >  1:351994/1‑101 (MQ=255)


                         gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                             <  2:3347745/101‑1 (MQ=255)


                         gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                             >  1:1477450/1‑101 (MQ=255)


                          cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                                            <  2:4304358/101‑1 (MQ=255)


                           gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                           <  1:2271012/101‑1 (MQ=255)


                            cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                          <  1:1034042/101‑1 (MQ=255)


                             ggccctcctCAGACGTCGCCCCCCCCGCGGGGAGTTGGATCGAAACA.GCCT.GCGCG.G..CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                                        <  1:4003723/98‑1 (MQ=38)


                                 ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  1:1780407/11‑101 (MQ=9)


                                 attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:2704551/11‑101 (MQ=0)


                                  gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  2:3607657/92‑1 (MQ=9)


                                  gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  1:477244/92‑1 (MQ=9)


                                  cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  2:4593709/9‑101 (MQ=2)


                                  cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     <  2:2701087/93‑1 (MQ=2)


                                   gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                    <  2:826403/94‑1 (MQ=2)


                                   ccccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  1:1919304/94‑1 (MQ=0)


                                    ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  >  2:3939412/8‑101 (MQ=2)


                                    cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  1:2026338/95‑1 (MQ=2)


                                    caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                   <  2:233688/94‑1 (MQ=9)


                                     aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:4392637/95‑1 (MQ=9)


                                      acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  1:782169/6‑101 (MQ=9)


                                       ccccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                  <  1:2345228/98‑1 (MQ=2)


                                       aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                               >  2:1756737/1‑101 (MQ=255)


                                        tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGGGTGGATCGaaa                                                               >  2:1783993/3‑101 (MQ=2)


                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCCCAACac                                                            >  2:3204231/1‑100 (MQ=2)


                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                            >  2:2680248/1‑99 (MQ=9)


                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                          <  2:2444381/101‑1 (MQ=255)


                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                                         >  1:1653918/1‑101 (MQ=255)


                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                         >  1:3830550/1‑96 (MQ=2)


                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                          >  1:572720/1‑97 (MQ=9)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                                           <  1:1346730/101‑3 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                         <  2:346236/101‑6 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                         <  1:2911496/101‑6 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                        >  1:97918/1‑101 (MQ=255)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                        <  1:3008021/101‑7 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                        <  2:523761/101‑7 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                        <  2:1113765/101‑7 (MQ=2)


                                               cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccgcca                                                        >  2:1186824/1‑94 (MQ=2)


                                                tCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                         <  2:3521576/101‑2 (MQ=2)


                                                tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                       >  1:2141991/1‑94 (MQ=9)


                                                 ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                        <  1:247915/101‑3 (MQ=2)


                                                 ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                     <  1:4485809/101‑10 (MQ=2)


                                                  cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                                       <  1:920291/101‑4 (MQ=2)


                                                  cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                                                    >  1:2614198/1‑91 (MQ=2)


                                                   ccccGCGGGGGCTTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTC..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                                   <  2:200086/101‑1 (MQ=255)


                                                   ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                                      <  2:2927158/101‑5 (MQ=2)


                                                   ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                                    >  1:421990/1‑91 (MQ=2)


                                                   ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCTGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                                   >  1:855830/1‑101 (MQ=255)


                                                   ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                    <  2:4425292/101‑11 (MQ=2)


                                                   ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                                    >  1:2507218/1‑91 (MQ=2)


                                                    cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                                     <  2:1212956/101‑6 (MQ=0)


                                                      cgggggggCGTGCATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                                <  2:1426817/98‑1 (MQ=255)


                                                        ggggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                              <  2:2189925/100‑1 (MQ=255)


                                                        cgGGGGGGTGGATCGAAACA.GCCT.GCCCG.GN.CGCA.TCGGCT.TCCTC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                              <  1:1231237/101‑1 (MQ=255)


                                                              cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                                        >  1:2408764/1‑101 (MQ=255)


                                                                tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                      >  2:3703090/1‑101 (MQ=255)


                                                                 ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     <  2:3776703/101‑1 (MQ=255)


                                                                 ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                                     >  2:1046852/1‑101 (MQ=255)


                                                                  gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                                    <  1:3735938/101‑1 (MQ=255)


                                                                     cGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                                 <  2:2494166/101‑1 (MQ=255)


                                                                       aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                               >  1:3480855/1‑101 (MQ=255)


                                                                        aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                              >  2:132336/1‑101 (MQ=255)


                                                                         aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                             <  1:3150124/101‑1 (MQ=255)


                                                                          cA.GCGT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                            <  2:2985757/101‑1 (MQ=255)


                                                                          cA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                            <  2:1251902/101‑1 (MQ=255)


                                                                           a.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGt                           <  2:2037908/101‑1 (MQ=255)


                                                                               cT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGGTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                        >  2:2538739/1‑101 (MQ=255)


                                                                               cT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGCCGGACGAGGTCCGTCGc                        >  2:414347/1‑101 (MQ=255)


                                                                                   cgcg.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc                     >  2:701828/1‑101 (MQ=255)


                                                                                        gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc                 <  2:4511398/101‑1 (MQ=255)


                                                                                            gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg              >  1:2214320/1‑101 (MQ=255)


                                                                                             cA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg             >  1:652429/1‑101 (MQ=255)


                                                                                              a.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg            >  2:3046191/1‑101 (MQ=255)


                                                                                                tCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg           <  1:4161544/101‑1 (MQ=255)


                                                                                                tCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgggg           <  1:393653/101‑1 (MQ=255)


                                                                                                 cgccT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggggg          <  2:1688783/98‑1 (MQ=255)


                                                                                                   gCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg        <  2:855754/101‑1 (MQ=255)


                                                                                                   gCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg        >  1:1973543/1‑101 (MQ=255)


                                                                                                    gtttgcGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCg        >  1:4317809/6‑101 (MQ=255)


                                                                                                     t.tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCggg      >  2:1386681/1‑99 (MQ=255)


                                                                                                       tCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGGgg     >  1:4574773/1‑101 (MQ=255)


                                                                                                         cGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGAt   <  1:2072126/101‑1 (MQ=255)


ATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396860‑1397060

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG