Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,013 T→C 15.8% M299M (TTG→CAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,0130T→C15.8% 95.7 ‑2.5 ‑1.1246M299M (TTG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/22):  ref base (122/85):  total (139/107)
Fisher's exact test strand distribution p-value = 8.13e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.07e-02

Only 100 of 284 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCG  >  NC_014034/1396913‑1397112
                                                                                                      |                                                                                                       
 cgcgGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGtt                                                                                                        >  1:378779/1‑101 (MQ=255)
    gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGt                                                                                                     >  2:2223901/1‑101 (MQ=255)
    gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGt                                                                                                     >  2:3149880/1‑101 (MQ=255)
    gggggCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGt                                                                                                     >  2:3471174/1‑101 (MQ=255)
        gCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGc                                                                                                 >  2:2040512/1‑101 (MQ=255)
         cGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGcc                                                                                                >  2:4261865/1‑101 (MQ=255)
          gTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCg                                                                                               >  1:2625043/1‑101 (MQ=255)
                   aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAATCATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                     >  1:4036891/1‑101 (MQ=255)
                   aaCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                                                                     >  2:3023395/1‑101 (MQ=255)
                    aCAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTcc                                                                                    <  1:3150124/101‑1 (MQ=255)
                     cAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...g                                                                                <  2:1251902/101‑1 (MQ=255)
                       gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTc                                                                              >  1:3512471/1‑101 (MQ=255)
                         cTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGc                                                                            >  2:461799/1‑101 (MQ=255)
                           gcgcGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTc                                                                          <  2:1653807/101‑1 (MQ=255)
                            cgcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                                         >  2:1320461/1‑101 (MQ=255)
                              cgGCCGCCTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCCGGC.GATGTCC...GTCGCTcccc                                                                       <  2:3296814/101‑1 (MQ=255)
                               ggCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccccc                                                                      >  2:3539394/1‑101 (MQ=255)
                                gCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccccc                                                                     <  2:2356756/101‑1 (MQ=255)
                                  cGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgc                                                                   >  2:2962206/1‑101 (MQ=255)
                                   gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                                  <  2:756917/101‑1 (MQ=255)
                                   gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                                  >  1:2411195/1‑101 (MQ=255)
                                      tCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCgggg                                                               <  1:4161544/101‑1 (MQ=255)
                                       cGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggggg                                                              <  2:1096522/101‑1 (MQ=255)
                                         gCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCg                                                            >  1:4543313/1‑101 (MQ=255)
                                             ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:462783/1‑101 (MQ=255)
                                              gatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTgg                                                         >  1:2302473/10‑101 (MQ=2)
                                              cGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  1:2072126/101‑1 (MQ=255)
                                               gtcccgccggTCGCTCCCGCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  1:1603174/93‑1 (MQ=2)
                                               gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      >  2:1954706/1‑101 (MQ=255)
                                               gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  1:1119958/101‑1 (MQ=255)
                                               gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGCGGATc                                                      >  1:85295/1‑101 (MQ=255)
                                               atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  2:240526/9‑101 (MQ=2)
                                                 cTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  2:4094920/1‑101 (MQ=255)
                                                  ccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     <  2:2653565/96‑1 (MQ=2)
                                                  agcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCG.CCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     >  2:251018/7‑101 (MQ=2)
                                                   gCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:375360/101‑1 (MQ=255)
                                                    cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:2136283/101‑1 (MQ=255)
                                                    cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:785648/101‑1 (MQ=255)
                                                    cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 >  1:4279885/1‑101 (MQ=255)
                                                     ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCG.CCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  1:523809/4‑101 (MQ=0)
                                                     ggcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCG.CCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:1396239/98‑1 (MQ=0)
                                                     ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:1806798/99‑1 (MQ=2)
                                                     ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:4353667/99‑1 (MQ=2)
                                                     ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:1034040/99‑1 (MQ=2)
                                                      tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                               <  1:907884/101‑1 (MQ=255)
                                                      tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                               >  1:3686622/1‑101 (MQ=255)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGTGGGCGTGGATCGAAAc                                                 <  1:4486819/100‑1 (MQ=2)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 >  2:2026198/2‑101 (MQ=2)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:1883622/100‑1 (MQ=2)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 >  1:1380751/2‑101 (MQ=2)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:3897068/100‑1 (MQ=2)
                                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                >  1:394476/1‑100 (MQ=2)
                                                       cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCG.CCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                >  2:581921/2‑100 (MQ=2)
                                                         gccccccccccGGGGGGGGGGGGAACCAAACAACAGGCCCG.T.GTTGGGTAGCCCGAC.GATGGCC...GGCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCCg                                            <  2:309393/96‑1 (MQ=9)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGt                                           >  1:3312953/1‑101 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                            >  2:1833530/1‑99 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                          <  2:2187362/101‑1 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCG.CCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                            >  1:1441731/1‑96 (MQ=2)
                                                           ccTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                          <  1:1222609/100‑1 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                          >  2:3662002/1‑97 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                        >  2:62121/1‑101 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAAC.CCG.CCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactgac                                          >  1:622795/1‑94 (MQ=2)
                                                             cccccccGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                          <  1:103860/100‑5 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                         <  1:2719672/101‑6 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTg                                       <  2:2735368/101‑1 (MQ=255)
                                                              ccccccGCAGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                         >  2:4522955/1‑96 (MQ=2)
                                                                cgccGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                     >  2:1880572/3‑101 (MQ=255)
                                                                ccccGCGGGGGCGTGGATCGAAACATCA.GCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatc                                       <  1:2253792/101‑8 (MQ=2)
                                                                ccccGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAg                                     <  1:1619865/101‑1 (MQ=255)
                                                                 cccGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGt                                    <  2:2293144/101‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                              >  2:706963/1‑101 (MQ=255)
                                                                       ggggCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                              <  1:1256621/101‑1 (MQ=255)
                                                                        gggCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgggg                             >  1:4113737/1‑101 (MQ=255)
                                                                           cGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgc                          >  1:1484964/1‑101 (MQ=255)
                                                                            gTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                         <  2:3222105/101‑1 (MQ=255)
                                                                             tGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGa                        <  2:3996950/101‑1 (MQ=255)
                                                                              ggATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAg                       <  2:2458696/101‑1 (MQ=255)
                                                                              ggATCGAAACATCA.GCTCG.TCGTTGGTAAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATAGAAACTGCCGTCTTGAGTTATGGGGCGCAAg                       >  2:4158622/1‑101 (MQ=38)
                                                                               gATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                      >  1:989637/1‑101 (MQ=255)
                                                                                 tCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGGGCGAGcac                    >  2:876812/1‑101 (MQ=255)
                                                                                  cGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca                   >  1:280764/1‑101 (MQ=255)
                                                                                   gAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                  >  2:1594751/1‑101 (MQ=255)
                                                                                    aaaCATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                 <  2:4330745/101‑1 (MQ=255)
                                                                                    aaaCATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATg                 >  1:2658734/1‑101 (MQ=255)
                                                                                     aaCACCT.GCACG.TCGTTGGTCAGGCGGAC.GATGTGC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                <  2:4536463/101‑1 (MQ=37)
                                                                                      aCATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtc               <  2:3698749/101‑1 (MQ=255)
                                                                                        ctCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt             <  2:85281/100‑1 (MQ=255)
                                                                                        aTCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt             <  1:2175456/101‑1 (MQ=255)
                                                                                        aTCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt             >  1:4244494/1‑101 (MQ=255)
                                                                                             gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt         <  1:4210281/101‑1 (MQ=255)
                                                                                               tcg.tcgTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcc       <  1:2393973/101‑1 (MQ=255)
                                                                                                cg.tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc      >  1:2017295/1‑101 (MQ=255)
                                                                                                 g.tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccc     >  2:1766061/1‑101 (MQ=255)
                                                                                                   tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc    <  1:2224063/101‑1 (MQ=255)
                                                                                                    cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc   >  1:3260647/1‑101 (MQ=255)
                                                                                                    cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc   >  1:1977417/1‑101 (MQ=255)
                                                                                                    cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc   <  2:4448888/101‑1 (MQ=255)
                                                                                                    cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc   >  1:2945225/1‑101 (MQ=255)
                                                                                                    cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc   <  1:2538913/101‑1 (MQ=255)
                                                                                                     gtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg  >  2:4468523/1‑101 (MQ=255)
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CCGCGGGGGCGTGGATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCA.GCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCG  >  NC_014034/1396913‑1397112

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG