Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,162 G→C 6.5% V348V (GTG→GCC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1620G→C 6.5% 26.7 0.0 ‑0.5215V348V (GTG→GTCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/7):  ref base (95/106):  total (102/113)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.03e-01

Only 100 of 244 total aligned reads displayed.

AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397062‑1397261
                                                                                                     |                                                                                                    
aaaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTg                                                                                                      >  2:3268915/1‑101 (MQ=255)
 aaCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgc                                                                                                     <  1:931211/101‑1 (MQ=255)
  aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcg                                                                                                    >  1:3758672/1‑101 (MQ=255)
  aCTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTgcc                                                                                                    >  1:1364818/1‑100 (MQ=255)
         tCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGc                                                                                             >  2:3663534/1‑101 (MQ=255)
          cTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCt                                                                                            >  1:3632454/1‑101 (MQ=255)
            tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcc                                                                                          >  1:523025/1‑101 (MQ=255)
             gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                         <  2:875024/101‑1 (MQ=255)
             gAGTGATGGGGCGCGAGCACATGTCGCCGCTCCCCCCGCGGGGGCGTGGATCGTAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                         <  2:4036606/101‑1 (MQ=255)
              aGTGATGGGGCGCGAGCCCATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                                        <  2:805363/101‑1 (MQ=255)
              aGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccc                                                                                        <  1:2955663/101‑1 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                       >  2:2424334/1‑101 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                       <  1:2372460/101‑1 (MQ=255)
               gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GAGCGCCGTCGCTccccc                                                                                       >  1:161347/1‑101 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                      >  1:2785702/1‑101 (MQ=255)
                tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                      <  1:23178/101‑1 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                     >  1:3686351/1‑101 (MQ=255)
                 gATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                     >  1:2518812/1‑101 (MQ=255)
                   tGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgcg                                                                                   >  1:2983925/1‑101 (MQ=255)
                    ggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCgg                                                                                  >  1:2121870/1‑101 (MQ=255)
                     gggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCggg                                                                                 >  1:3165702/1‑101 (MQ=255)
                        cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                                              >  2:1088349/1‑101 (MQ=255)
                         gctaGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCg                                                                             <  2:594348/98‑1 (MQ=255)
                         gcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCg                                                                             >  1:1531685/1‑101 (MQ=255)
                          cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGt                                                                            >  2:4001553/1‑101 (MQ=255)
                          cgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGt                                                                            <  2:4560179/101‑1 (MQ=255)
                           gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:672399/1‑101 (MQ=255)
                           gAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:1983278/101‑1 (MQ=255)
                              cggctgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:2007450/93‑1 (MQ=0)
                              cacaTGTCGTCGCTGCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  1:4036465/101‑1 (MQ=255)
                              cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:4087144/101‑1 (MQ=255)
                              cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:2226353/101‑1 (MQ=255)
                              cacaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:3481830/101‑1 (MQ=255)
                               acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:2844813/101‑1 (MQ=255)
                                 aTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:672461/101‑1 (MQ=255)
                                  tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:1507668/97‑1 (MQ=2)
                                  tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:1920896/97‑1 (MQ=2)
                                  tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:772335/5‑101 (MQ=2)
                                  tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:3114752/1‑101 (MQ=255)
                                  tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:938555/101‑1 (MQ=255)
                                  tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  2:281380/1‑101 (MQ=255)
                                   gcggtcgCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:130476/98‑1 (MQ=2)
                                     ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                 >  2:1545564/2‑100 (MQ=2)
                                     ggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                 >  1:443637/2‑100 (MQ=2)
                                       tcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtg                                                               >  1:1142146/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                            <  2:1413181/101‑1 (MQ=255)
                                           ctcccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                                           <  1:1753730/99‑1 (MQ=255)
                                           cccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                                           <  1:3430785/100‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                          <  2:3965394/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                          <  2:3632186/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGc                                                          >  2:1287847/1‑101 (MQ=255)
                                               cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.c                                                      >  1:2693422/1‑101 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                     <  2:482053/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                     <  2:4436551/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                   <  1:3269160/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                   <  2:3079885/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                 >  1:636298/1‑101 (MQ=255)
                                                       ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                              <  1:3538228/101‑1 (MQ=255)
                                                       ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                              >  1:4333536/1‑101 (MQ=255)
                                                        gcggttaTCGAACCGTCCAGCGGGCGTCGAATGTTTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTg                                             <  2:633901/95‑1 (MQ=21)
                                                           tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAc                                          <  1:1594856/101‑1 (MQ=255)
                                                           tGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAc                                          >  2:1372339/1‑101 (MQ=255)
                                                           ctgATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAc                                          <  1:3062956/99‑1 (MQ=255)
                                                            ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                         <  2:3853943/101‑1 (MQ=255)
                                                             gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCa                                        >  2:414064/1‑101 (MQ=255)
                                                              aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAg                                       <  2:2689353/101‑1 (MQ=255)
                                                                cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCCGAt                                     >  2:1703813/1‑101 (MQ=255)
                                                                 gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                    >  2:2721376/1‑101 (MQ=255)
                                                                  aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                   >  1:3242167/1‑101 (MQ=255)
                                                                   aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                  <  1:2040652/101‑1 (MQ=255)
                                                                    aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACGCGTCTGCCA.CGACGCCCTGCACCAGATCcgc                                 <  1:4145751/101‑1 (MQ=255)
                                                                    aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCGGATCcgc                                 >  1:4086263/1‑101 (MQ=255)
                                                                       tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                              >  1:271669/1‑101 (MQ=255)
                                                                       tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                              <  1:4578381/101‑1 (MQ=255)
                                                                         cAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGt                            <  1:2250011/101‑1 (MQ=255)
                                                                          cgCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                           <  1:2620566/100‑1 (MQ=255)
                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                           <  1:1027333/101‑1 (MQ=255)
                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                           >  2:2474732/1‑101 (MQ=255)
                                                                          aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                           >  2:1136019/1‑101 (MQ=255)
                                                                           gCTCGCGCCACAGGTCTTCGACC.GTGCGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCg                          <  2:1504542/101‑1 (MQ=255)
                                                                            cTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGc                         >  1:1475467/1‑101 (MQ=255)
                                                                             tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                        <  2:3312704/101‑1 (MQ=255)
                                                                             tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CAACGCCCTGCACCAGATCCGCGCCGTCGCt                        >  2:755258/1‑101 (MQ=255)
                                                                             tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCACCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                        <  1:4014290/101‑1 (MQ=255)
                                                                                cgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                     <  1:858148/101‑1 (MQ=255)
                                                                                cgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                     >  2:1240039/1‑101 (MQ=255)
                                                                                 gcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc                    >  2:4197792/1‑101 (MQ=255)
                                                                                   cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                  <  1:970237/101‑1 (MQ=255)
                                                                                   cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                  <  2:378742/101‑1 (MQ=255)
                                                                                   cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                  >  2:3106214/1‑101 (MQ=255)
                                                                                     caTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgc                >  2:4627473/1‑101 (MQ=255)
                                                                                        gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCC.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg             >  2:3973961/1‑101 (MQ=255)
                                                                                         tCTTCGCCC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg            <  2:2112615/101‑1 (MQ=255)
                                                                                           ttCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc          >  1:1767604/1‑101 (MQ=255)
                                                                                            tCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCg         >  1:1113752/1‑101 (MQ=255)
                                                                                             cGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCgg        >  1:4479198/1‑100 (MQ=255)
                                                                                               aCC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg      <  2:1767485/101‑1 (MQ=255)
                                                                                                cc.GTGCGCCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa     <  2:4279597/101‑1 (MQ=255)
                                                                                                    tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:2915556/101‑1 (MQ=255)
                                                                                                     gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  2:1403129/101‑1 (MQ=2)
                                                                                                     |                                                                                                    
AAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397062‑1397261

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG