Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,291 C→A 3.3% S391R (AGC→GGA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2910C→A 3.3% 8.0 0.0 ‑0.0210S391R (AGC→AGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (100/103):  total (103/107)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.88e-01

Only 100 of 235 total aligned reads displayed.

ATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397191‑1397391
                                                                                                      |                                                                                                    
aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGc                                                                                                      >  2:25788/1‑101 (MQ=255)
aTCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGc                                                                                                      >  1:3455723/1‑101 (MQ=255)
  cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                                    >  1:75549/1‑101 (MQ=255)
  cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GGCAGccc                                                                                                    >  1:63327/1‑101 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                   <  1:1088438/101‑1 (MQ=255)
   gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                                   >  1:2306992/1‑101 (MQ=255)
    aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                                  >  1:3411801/1‑101 (MQ=255)
       cGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGa                                                                                               >  1:479587/1‑101 (MQ=255)
       cGTGGCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGa                                                                                               <  2:4508229/101‑1 (MQ=255)
        gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                              >  2:4268317/1‑101 (MQ=255)
        gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                              <  2:3165458/101‑1 (MQ=255)
          gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                                            >  2:4238100/1‑101 (MQ=255)
          gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                                            >  1:120801/1‑101 (MQ=255)
            cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGACCGTc                                                                                          >  2:653502/1‑101 (MQ=255)
            cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                                          <  2:3252108/101‑1 (MQ=255)
             tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCg                                                                                         <  1:140490/101‑1 (MQ=255)
               ccACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCt                                                                                       <  1:3618659/101‑1 (MQ=255)
                 acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                     >  2:4154511/1‑101 (MQ=255)
                 acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                     >  1:3866418/1‑101 (MQ=255)
                  cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                    <  2:2092694/101‑1 (MQ=255)
                  cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                    <  2:1137387/101‑1 (MQ=255)
                   gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                                                                                   >  2:3385227/1‑101 (MQ=255)
                     cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                 <  1:1861510/101‑1 (MQ=255)
                     cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                 <  2:4478903/101‑1 (MQ=255)
                      gCCCTGCCCCAGATCCGCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                                                                                <  1:3964973/101‑1 (MQ=255)
                      gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                                                                                >  2:4052332/1‑101 (MQ=255)
                      gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                                                                                >  2:4330662/1‑101 (MQ=255)
                         cTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCgg                                                                             >  2:2831036/1‑101 (MQ=255)
                           gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                           <  2:936958/101‑1 (MQ=255)
                            cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                          >  2:2484313/1‑101 (MQ=255)
                             cccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                         <  2:1977282/100‑1 (MQ=255)
                             aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCTCGGGAGc                                                                         >  2:2069662/1‑101 (MQ=255)
                             aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                         >  1:4056694/1‑101 (MQ=255)
                                ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                      <  2:2128452/91‑1 (MQ=2)
                                ggccctcgcacGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                      <  2:1320627/91‑1 (MQ=2)
                                aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                                      >  2:1454300/1‑101 (MQ=255)
                                 gATCGGCTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGATCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                     <  2:2982316/101‑1 (MQ=37)
                                  aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa                                                                    >  1:3268872/1‑101 (MQ=255)
                                  aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGA.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                    >  1:4620559/1‑101 (MQ=255)
                                   tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAt                                                                   <  1:2607590/101‑1 (MQ=255)
                                   tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAt                                                                   <  1:4013739/101‑1 (MQ=255)
                                    ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                  <  2:2443033/101‑1 (MQ=255)
                                    ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                  <  2:119085/101‑1 (MQ=255)
                                    ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCATGGATc                                                                  >  2:1008595/1‑101 (MQ=255)
                                     cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                 <  2:3692258/101‑1 (MQ=255)
                                     cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                 <  1:1141819/101‑1 (MQ=255)
                                      gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                >  1:1943787/1‑101 (MQ=255)
                                      gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                <  2:3585997/101‑1 (MQ=255)
                                       cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                               <  2:2693226/101‑1 (MQ=255)
                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                            >  2:2766710/1‑101 (MQ=255)
                                          cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                            >  2:3885226/1‑101 (MQ=255)
                                          cGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                            <  2:1484855/101‑1 (MQ=255)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                           >  2:2617040/1‑101 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGACGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                          >  1:4033436/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                         >  2:3797580/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                        <  2:75543/101‑1 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                        >  2:3766375/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                        >  1:4516705/1‑101 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                        >  2:1708676/1‑101 (MQ=255)
                                               ccccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                       <  1:25793/99‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                     >  1:4452474/1‑101 (MQ=255)
                                                  cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                                    <  1:1708787/101‑1 (MQ=255)
                                                     ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGTGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                 >  2:2460396/1‑92 (MQ=0)
                                                     ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                 <  2:1614773/101‑10 (MQ=0)
                                                      cgcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCt                                                <  1:3093508/101‑1 (MQ=255)
                                                       gcgGGGGCGTGGATCGAAACGCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                               <  1:3131291/101‑1 (MQ=255)
                                                       gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTATAGCCtc                                               >  2:851699/1‑100 (MQ=255)
                                                       gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                               <  1:1372434/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                             >  2:4367897/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                             <  1:2387283/101‑1 (MQ=255)
                                                          ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                            <  1:4629642/101‑1 (MQ=255)
                                                            ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                          >  2:1802699/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                          <  2:1530751/101‑1 (MQ=255)
                                                            ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                                          <  2:4188680/101‑1 (MQ=255)
                                                             gCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcc                                         >  2:2354799/1‑101 (MQ=255)
                                                                 ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGTGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                     <  2:2378911/101‑1 (MQ=255)
                                                                   aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCCCGc                                   >  1:3296661/1‑101 (MQ=255)
                                                                   aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                                   >  2:3539109/1‑101 (MQ=255)
                                                                     cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                 <  2:120787/101‑1 (MQ=255)
                                                                      gAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                                <  1:3750765/101‑1 (MQ=255)
                                                                              gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                        <  1:1526164/101‑1 (MQ=255)
                                                                                 gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                      <  2:2248389/101‑1 (MQ=255)
                                                                                 gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                      >  2:1885703/1‑101 (MQ=255)
                                                                                 gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                      >  2:1001297/1‑101 (MQ=255)
                                                                                 gcACGCGCGAACGGC.GTCAGCCCCGCGAACGGCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCCGACGGACGTcc                      >  1:2744751/1‑100 (MQ=255)
                                                                                 gcACGCCCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                      <  1:2101877/101‑1 (MQ=255)
                                                                                  cACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc                     >  1:3258160/1‑101 (MQ=255)
                                                                                      cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc                 >  1:2571660/1‑101 (MQ=255)
                                                                                         gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc              <  1:2856031/101‑1 (MQ=255)
                                                                                          aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg             <  1:3202019/101‑1 (MQ=255)
                                                                                             ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg          <  1:3853278/101‑1 (MQ=255)
                                                                                              gccgccggCCCCGCGACCGTCGCGCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg          <  2:3979135/94‑1 (MQ=38)
                                                                                                 gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg       >  2:4642789/1‑101 (MQ=255)
                                                                                                 gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCCCggggg       >  1:2214928/1‑101 (MQ=255)
                                                                                                  tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc      <  1:414384/101‑1 (MQ=255)
                                                                                                   cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg     >  1:72042/1‑101 (MQ=255)
                                                                                                   cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg     <  2:2646337/101‑1 (MQ=255)
                                                                                                   cAGCCCCGCGAACGTCGCCCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg     <  1:4198080/101‑1 (MQ=255)
                                                                                                    aGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCgg    >  1:2430555/1‑100 (MQ=255)
                                                                                                      ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg  <  1:2354961/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
ATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397191‑1397391

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG