Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,318 A→G 4.1% G400G (GGA→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3180A→G 4.1% 13.4 0.0 ‑1.7219G400G (GGA→GGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/5):  ref base (88/122):  total (92/127)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.85e-02

Only 100 of 238 total aligned reads displayed.

GCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397218‑1397418
                                                                                                      |                                                                                                     
 cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                                                      >  2:2484313/1‑101 (MQ=255)
  cccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                                                     <  2:1977282/100‑1 (MQ=255)
  aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                                                     >  1:201206/1‑101 (MQ=255)
    cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                                                   <  1:1482345/101‑1 (MQ=255)
     ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                                                  <  2:2128452/91‑1 (MQ=2)
     aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                                                                  >  2:1454300/1‑101 (MQ=255)
      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                                                 <  1:4540257/101‑1 (MQ=255)
       aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGA.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                                                >  1:4620559/1‑101 (MQ=255)
        tCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAt                                                                                               <  2:2017155/101‑1 (MQ=255)
         ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                                              <  2:2443033/101‑1 (MQ=255)
         ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCATGGATc                                                                                              >  2:1008595/1‑101 (MQ=255)
          cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                                             <  1:1141819/101‑1 (MQ=255)
           gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                                            <  2:3966783/101‑1 (MQ=255)
           gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                                            >  2:24128/1‑101 (MQ=255)
           gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                                            >  1:1943787/1‑101 (MQ=255)
               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                                        >  2:2766710/1‑101 (MQ=255)
               cGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                                        <  2:1484855/101‑1 (MQ=255)
                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                                       >  2:2617040/1‑101 (MQ=255)
                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAAgg                                                                                       >  1:2379073/1‑101 (MQ=255)
                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                                      >  1:257602/1‑101 (MQ=255)
                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                                     <  2:144956/101‑1 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  2:3766375/1‑101 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  2:1708676/1‑101 (MQ=255)
                    cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                                                   >  2:3777662/1‑101 (MQ=255)
                     cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.a                                                                                 <  1:4020345/100‑1 (MQ=255)
                      ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                >  1:3664093/1‑101 (MQ=255)
                       cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACa                                                                               <  1:1708787/101‑1 (MQ=255)
                         cccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGc                                                                             <  2:3292836/101‑1 (MQ=255)
                          ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                                             <  2:1614773/101‑10 (MQ=0)
                          ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                                             >  2:2178612/1‑92 (MQ=0)
                           cgcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCt                                                                           <  1:3093508/101‑1 (MQ=255)
                            gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ATAGCCtc                                                                          >  2:851699/1‑100 (MQ=255)
                            gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCtt                                                                          <  1:1372434/101‑1 (MQ=255)
                            gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCtt                                                                          >  2:4328577/1‑101 (MQ=255)
                             cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                         >  1:3358813/1‑101 (MQ=255)
                              gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                        >  2:4367897/1‑101 (MQ=255)
                              gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.AAAGCCTTcg                                                                        >  2:2101737/1‑101 (MQ=255)
                               ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgc                                                                       <  1:4629642/101‑1 (MQ=255)
                                gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                      <  1:4052617/101‑1 (MQ=255)
                                 ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCgcgc                                                                     <  2:1530751/101‑1 (MQ=255)
                                     tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 >  2:1184583/1‑101 (MQ=255)
                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccccc                                                                >  2:2362525/1‑99 (MQ=255)
                                      ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                <  2:4071730/101‑1 (MQ=255)
                                       gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               >  2:4208451/1‑101 (MQ=255)
                                        aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACTACCTTCGCGCCCCACGc                                                              >  2:4185518/1‑101 (MQ=255)
                                         tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                             >  2:2935462/1‑101 (MQ=255)
                                          cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            <  2:1872221/101‑1 (MQ=255)
                                          cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            <  2:120787/101‑1 (MQ=255)
                                               cacCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAACCTTCGCGCCCCACCCCAgacgg                                                       >  2:1842964/1‑101 (MQ=255)
                                                 ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                     <  2:479539/101‑1 (MQ=255)
                                                   gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                   <  2:4504291/101‑1 (MQ=255)
                                                      gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                                                 >  1:1199583/1‑101 (MQ=255)
                                                         cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                                              <  2:3686076/101‑1 (MQ=255)
                                                             cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc                                          >  1:2148411/1‑101 (MQ=255)
                                                              gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                         <  1:2856031/101‑1 (MQ=255)
                                                               aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                                        <  1:3202019/101‑1 (MQ=255)
                                                               aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                                        <  2:3091255/101‑1 (MQ=255)
                                                                aCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcgc                                       >  1:2308827/1‑101 (MQ=255)
                                                                  ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                                     <  1:3853278/101‑1 (MQ=255)
                                                                  ggC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg                                     <  2:3576996/101‑1 (MQ=255)
                                                                    c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                   >  1:2592309/1‑101 (MQ=255)
                                                                      gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCCCggggg                                  >  1:2214928/1‑101 (MQ=255)
                                                                        cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg                                >  1:72042/1‑101 (MQ=255)
                                                                        cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg                                <  2:2646337/101‑1 (MQ=255)
                                                                         aGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCgg                               >  1:2430555/1‑100 (MQ=255)
                                                                         aGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACCCCAGACGGACGTCGCTCCCCCCGCGGGGCCgc                               >  1:3267203/1‑100 (MQ=255)
                                                                          gCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTg                              <  1:2611027/101‑1 (MQ=255)
                                                                             ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt                           <  1:656995/101‑1 (MQ=255)
                                                                             ccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGAACGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt                           <  1:4000469/101‑1 (MQ=255)
                                                                             ccGCGAACGTCGCGCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGAt                           <  1:3974246/101‑1 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  2:2223896/101‑1 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  2:4565162/101‑1 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  2:653697/101‑1 (MQ=255)
                                                                              cgcgAACGGCGCCCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  2:1300810/101‑1 (MQ=255)
                                                                               gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                         <  1:993556/101‑1 (MQ=255)
                                                                                cgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                        >  2:3587647/1‑101 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       <  2:271637/101‑1 (MQ=255)
                                                                                  aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                      <  2:3983586/101‑1 (MQ=255)
                                                                                      tCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                  <  1:3777937/101‑1 (MQ=255)
                                                                                      gcGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                  <  2:2306830/100‑1 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 >  1:4072814/1‑101 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 <  1:1558369/101‑1 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACTCtt                 >  1:3131235/1‑101 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                >  1:139468/1‑101 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                <  1:275239/101‑1 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccctc                >  2:827629/1‑96 (MQ=255)
                                                                                         cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa               <  1:4238388/101‑1 (MQ=255)
                                                                                           ccccccGCGGGAGCGTGGATCGAAGCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt             <  2:562270/101‑1 (MQ=255)
                                                                                           ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCCGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAAt             <  2:4033151/101‑1 (MQ=255)
                                                                                            cccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATg            <  1:3431782/101‑1 (MQ=255)
                                                                                            cccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATg            <  1:4185806/101‑1 (MQ=255)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          >  2:3534984/1‑91 (MQ=2)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      >  1:2744595/1‑101 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTG.ACAGCCTTCGCACCCCACGCAAGACGGACGTCGCACCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      <  2:2047121/101‑1 (MQ=38)
                                                                                                   gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTCTTCAATGGATATgg     >  2:3873653/1‑101 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    <  1:4332635/101‑1 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    <  1:1893408/101‑1 (MQ=255)
                                                                                                     gAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCa   >  1:3272915/1‑101 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  <  2:3410103/101‑1 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  <  1:1001380/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
GCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397218‑1397418

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG