Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,476 C→A 14.4% T453N (ACC→AAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4760C→A14.4% 94.1 ‑0.3 0.0256T453N (ACC→AAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (18/19):  ref base (116/103):  total (134/122)
Fisher's exact test strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCC  >  NC_014034/1397376‑1397576
                                                                                                      |                                                                                                     
ccccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.ac                                                                                                       <  2:2412066/101‑1 (MQ=255)
 cccGAGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                                      <  1:4139572/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acca                                                                                                     <  1:2831252/101‑1 (MQ=255)
   cgcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.accac                                                                                                    <  1:1233358/101‑1 (MQ=255)
    gcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACg                                                                                                   >  1:1918000/1‑101 (MQ=255)
      gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                                 >  1:2469420/1‑101 (MQ=255)
      gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                                 <  1:851775/101‑1 (MQ=255)
        gggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.c                                                                                              >  2:157749/1‑101 (MQ=255)
         ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.cc                                                                                             <  1:1885829/101‑1 (MQ=255)
         ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.CCATC.ACCACGCAA.cc                                                                                             >  2:2559721/1‑101 (MQ=255)
         ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAAACGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCACAG.CCAAC.ACCACGCAA.cc                                                                                             >  2:3282810/1‑101 (MQ=38)
          gCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCg                                                                                            <  2:1672134/101‑1 (MQ=255)
          cgtgtgATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCg                                                                                            >  2:1906694/6‑101 (MQ=255)
             tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGccc                                                                                         <  2:2025561/101‑1 (MQ=255)
             tGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGccc                                                                                         <  2:1190888/101‑1 (MQ=255)
              ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                        >  1:116073/1‑101 (MQ=255)
              ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                        >  1:3011685/1‑101 (MQ=255)
                  cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcac                                                                                    >  2:4257060/1‑101 (MQ=255)
                   gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacc                                                                                   >  2:3994350/1‑101 (MQ=255)
                    aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                  >  1:144264/1‑101 (MQ=255)
                    aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                  >  1:4222761/1‑101 (MQ=255)
                      aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                >  2:965055/1‑101 (MQ=255)
                       ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                               <  1:4368189/101‑1 (MQ=255)
                         ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                             <  2:3322449/101‑1 (MQ=255)
                         ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                             <  2:2209123/101‑1 (MQ=255)
                         ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                             >  1:3382523/1‑101 (MQ=255)
                         ctTCAATGGATATGGCAAAACCGTACGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                             <  1:4528589/101‑1 (MQ=255)
                          ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                            <  1:3953511/101‑1 (MQ=255)
                          ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                            >  1:1125728/1‑101 (MQ=255)
                           tCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCg                                                                           >  2:1045489/1‑101 (MQ=255)
                            cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                          <  2:2350885/101‑1 (MQ=255)
                            cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                          >  2:1765738/1‑101 (MQ=255)
                            cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                          >  1:3230188/1‑101 (MQ=255)
                             aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                         >  1:2372495/1‑101 (MQ=255)
                             aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                         <  1:120773/101‑1 (MQ=255)
                             aaTGGATAGGGCAAAACCGTCCGCGACATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                         <  1:3153146/101‑1 (MQ=255)
                              aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                        >  2:3038784/1‑101 (MQ=255)
                              aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                        >  1:202947/1‑101 (MQ=255)
                                 gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTcccc                                                                     >  2:591482/1‑101 (MQ=255)
                                  atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTccccc                                                                    >  1:3738694/1‑101 (MQ=255)
                                  atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTccccc                                                                    <  2:2678479/101‑1 (MQ=255)
                                   tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTcccccc                                                                   <  2:3189238/101‑1 (MQ=255)
                                    atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                  <  2:1924332/101‑1 (MQ=255)
                                     tGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgc                                                                 <  2:1347622/101‑1 (MQ=255)
                                      ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgcg                                                                <  1:1539989/101‑1 (MQ=255)
                                         aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgggg                                                             >  1:528065/1‑101 (MQ=255)
                                         aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCgggg                                                             <  2:3759825/101‑1 (MQ=255)
                                          aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                            >  2:4544727/1‑101 (MQ=255)
                                          aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                            >  2:410429/1‑101 (MQ=255)
                                           aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGc                                                           <  1:3313883/101‑1 (MQ=255)
                                              cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                        <  1:4257348/101‑1 (MQ=255)
                                               gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  2:1269005/101‑1 (MQ=255)
                                                tCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                      <  2:3266958/101‑1 (MQ=255)
                                                ccgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTg                                                        <  2:2099562/91‑1 (MQ=0)
                                                ccgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTg                                                        >  2:4335789/11‑101 (MQ=0)
                                                 cgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTgg                                                       >  2:3633653/10‑101 (MQ=0)
                                                   gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   >  1:1045310/1‑101 (MQ=255)
                                                   gcgACATGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:3246454/101‑1 (MQ=255)
                                                   ataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCCATC.GTCGCTCCCCCCGCGGGGGCGGggcc                                                     >  1:431979/8‑99 (MQ=0)
                                                    taccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGATc                                                    >  2:4016980/7‑101 (MQ=0)
                                                        gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                >  1:2929118/3‑101 (MQ=2)
                                                        gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                >  2:2717086/3‑101 (MQ=2)
                                                        aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                              >  2:3924702/1‑101 (MQ=255)
                                                        aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCA.TCATC.ACCACGCAA.CCGGCATGCACCACCTGGCGCTCCCCCCGCGGCGGCGCGGATCGAaaca                                              >  2:230504/1‑101 (MQ=25)
                                                          gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacg                                              >  2:3576413/1‑100 (MQ=2)
                                                          ctCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacg                                              <  1:4040175/100‑2 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggt                                            >  2:4616541/1‑98 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggg                                            >  2:3823899/1‑98 (MQ=2)
                                                              ccccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgt                                          <  2:3531662/99‑6 (MQ=2)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgt                                          >  2:2864458/1‑96 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                       >  1:2188528/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                      >  2:2533829/1‑101 (MQ=255)
                                                                 cccccGCGGGGGCGTGGTTCGAAACATCGATGATCAAACCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                       <  2:4057666/101‑9 (MQ=2)
                                                                 cccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCa                                     <  1:410466/101‑1 (MQ=255)
                                                                  ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                    <  2:4182587/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                  >  1:2076417/1‑101 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                  <  2:760505/101‑1 (MQ=255)
                                                                    ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAGgg                                  >  1:4556523/1‑101 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                <  2:3093492/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                >  1:1641126/1‑101 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                               <  1:620070/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                               >  1:1106602/1‑101 (MQ=255)
                                                                        gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                              <  2:3382273/101‑1 (MQ=255)
                                                                          gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                            <  1:3106458/101‑1 (MQ=255)
                                                                              gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                        >  1:2976212/1‑101 (MQ=255)
                                                                               cgatctgAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                       <  1:1847999/95‑1 (MQ=255)
                                                                                    cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                  >  1:2110832/1‑101 (MQ=255)
                                                                                      aaaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgc                >  2:1902633/1‑101 (MQ=255)
                                                                                        acacCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTg              >  2:3500112/1‑101 (MQ=255)
                                                                                           ccG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACg           <  1:2678088/101‑1 (MQ=255)
                                                                                            cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt          >  1:1728192/1‑101 (MQ=255)
                                                                                            cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt          >  1:2712480/1‑101 (MQ=255)
                                                                                               tcatc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCg        >  1:1592378/1‑101 (MQ=255)
                                                                                                 atc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt      <  2:168292/101‑1 (MQ=255)
                                                                                                  tc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTc     >  2:680126/1‑101 (MQ=255)
                                                                                                   c.accgcGCAC.CCGTCCTCCCCCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc    <  2:2744553/101‑1 (MQ=37)
                                                                                                   c.accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc    >  1:1758253/1‑101 (MQ=255)
                                                                                                     accccGCAC.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc   <  2:601023/101‑1 (MQ=255)
                                                                                                     accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc   <  1:3533878/101‑1 (MQ=255)
                                                                                                      ccacGNNA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc  <  1:2327019/101‑1 (MQ=255)
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CCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCC  >  NC_014034/1397376‑1397576

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG