Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,544 G→T 12.3% A476S (GCC→TAG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5440G→T12.3% 72.8 ‑1.3 ‑0.0270A476S (GCC→TCC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/20):  ref base (117/116):  total (131/139)
Fisher's exact test strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 291 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCC  >  NC_014034/1397444‑1397644
                                                                                                    |                                                                                                         
ccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAGgg                                                                                                           >  1:4556523/1‑101 (MQ=255)
   cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c..c                                                                                                     >  1:1106602/1‑101 (MQ=255)
   cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c..c                                                                                                     <  1:620070/101‑1 (MQ=255)
        gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                <  2:1917865/101‑1 (MQ=255)
          gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                              >  1:2949694/1‑101 (MQ=255)
            ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                            >  1:1866465/1‑101 (MQ=255)
                     cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                  >  2:2397761/1‑101 (MQ=255)
                        cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGt                                                                              >  1:2712480/1‑101 (MQ=255)
                          tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCg                                                                            >  1:1592378/1‑101 (MQ=255)
                              caccgcGCACCCGTCCTCCCCCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                        <  2:2744553/101‑1 (MQ=37)
                               accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                       <  1:3533878/101‑1 (MQ=255)
                                ccacGNNACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                      <  1:2327019/101‑1 (MQ=255)
                                 cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                     <  2:942914/101‑1 (MQ=255)
                                 cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                     <  2:201182/101‑1 (MQ=255)
                                  ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                    <  2:2214766/100‑1 (MQ=255)
                                  ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                    <  2:759481/100‑1 (MQ=255)
                                  ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                    <  2:4172688/100‑1 (MQ=255)
                                    gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                  >  1:3735399/1‑101 (MQ=255)
                                       aCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggg                                                               <  1:1902768/101‑1 (MQ=255)
                                            ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGt                                                          <  1:3101167/101‑1 (MQ=255)
                                             cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                         >  1:1805772/1‑101 (MQ=255)
                                              tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                        >  1:2096380/1‑101 (MQ=255)
                                               gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:3517195/101‑1 (MQ=255)
                                                 atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  1:718833/8‑101 (MQ=0)
                                                 acaggatggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:3886349/92‑1 (MQ=2)
                                                  gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  2:572672/96‑1 (MQ=0)
                                                  gcgctggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:2837856/96‑1 (MQ=0)
                                                    gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                   >  1:786080/5‑101 (MQ=0)
                                                     tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:2065067/98‑1 (MQ=2)
                                                     gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAc                                                >  1:3143688/4‑101 (MQ=2)
                                                     ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  2:3984654/3‑101 (MQ=2)
                                                      gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:2460562/99‑1 (MQ=2)
                                                      cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                <  2:4221004/101‑1 (MQ=255)
                                                       ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCCCGGGGGCGTGGATCGAAAcc                                               >  1:1224676/1‑100 (MQ=255)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggt                                            <  1:2166338/101‑5 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                             <  1:3926598/101‑1 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                             >  1:436994/1‑101 (MQ=255)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGTGGGTCGAAAcggggc                                           >  1:3562191/1‑96 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                            <  1:1673210/101‑2 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGGGGATCGACACATcg                                            >  2:2297310/1‑100 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                             >  1:3717322/1‑99 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                             >  1:2747654/1‑99 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                          <  2:851291/101‑7 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                          <  2:317070/101‑7 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                            <  2:2360360/101‑4 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                            >  1:2784935/1‑98 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATccc                                           >  2:1401004/1‑100 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                           <  1:1487832/101‑1 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                           <  2:1866339/101‑1 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                          >  1:202806/1‑98 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                           <  1:3500734/101‑5 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                          >  1:3344850/1‑101 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                          >  2:280529/1‑101 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                         >  2:1088950/1‑97 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                         >  1:410738/1‑97 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                         >  2:3078909/1‑97 (MQ=2)
                                                             cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                        <  2:3617962/100‑9 (MQ=2)
                                                             cccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                         <  2:2928646/100‑1 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                         >  1:1347954/1‑95 (MQ=0)
                                                               cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcgaaga                                      >  1:1981652/1‑91 (MQ=0)
                                                               cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcgaaaa                                      >  1:1729388/1‑91 (MQ=2)
                                                               cccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                       >  2:1327648/1‑101 (MQ=255)
                                                                ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATCC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACATcgatgatc                                      <  1:4617109/101‑8 (MQ=2)
                                                                ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                      >  1:2610532/1‑94 (MQ=2)
                                                                 cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                     <  1:3424577/101‑9 (MQ=0)
                                                                   cgcgGGGGCGTGGATCGNAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                   <  2:1591307/101‑1 (MQ=255)
                                                                   cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                   >  2:3270514/1‑101 (MQ=255)
                                                                   cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                   >  1:2494607/1‑101 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                 <  2:1828725/101‑1 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                 >  2:4511269/1‑101 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                 <  2:4320592/101‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                               <  1:3593312/101‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                               >  2:152396/1‑101 (MQ=255)
                                                                        gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTgc                              >  2:2104938/1‑100 (MQ=255)
                                                                        ccgatctgATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                              <  2:3381340/94‑1 (MQ=255)
                                                                         ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGACGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                             >  2:714505/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCACCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                             <  1:3994635/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                            <  1:2873334/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                            <  2:2188378/101‑1 (MQ=255)
                                                                           cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                           <  2:3457423/101‑1 (MQ=255)
                                                                           cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGCGGCGTGGATCGAAACACCCGCGGCTCGGCGTGAccc                           >  2:4434893/1‑101 (MQ=255)
                                                                            gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGGGCCGCGCT.GA.CGACGCTCCCCCCGCGGGTGCGTGGATCCAAACAACCGACGTTCTGCGGGAcccg                          >  2:174330/1‑101 (MQ=255)
                                                                            gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                          >  2:2517243/1‑101 (MQ=255)
                                                                             tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                         >  1:655630/1‑101 (MQ=255)
                                                                               gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcgg                       >  1:3381590/1‑94 (MQ=255)
                                                                               gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                       >  2:4074752/1‑101 (MQ=255)
                                                                                aTCGAACCAACCCTATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCCCCGGATCTGCGTGAcccgcccc                      <  2:1224584/101‑1 (MQ=38)
                                                                                 tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCGTCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                     <  1:4005618/101‑1 (MQ=255)
                                                                                       caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc               >  1:4302263/1‑101 (MQ=255)
                                                                                          cccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt            >  1:3823514/1‑101 (MQ=255)
                                                                                           ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc           <  2:102441/101‑1 (MQ=255)
                                                                                           ccGACCAGGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc           <  1:425616/101‑1 (MQ=255)
                                                                                            cGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg          <  1:2986720/101‑1 (MQ=255)
                                                                                              aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt        >  2:2664275/1‑101 (MQ=255)
                                                                                              aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt        <  2:1125639/101‑1 (MQ=255)
                                                                                              aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt        <  2:3893709/101‑1 (MQ=255)
                                                                                                cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGCCGCTcc      >  2:4669674/1‑101 (MQ=255)
                                                                                                 aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc     >  2:4614636/1‑101 (MQ=255)
                                                                                                   ggC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc   >  1:2652582/1‑101 (MQ=255)
                                                                                                   ggC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCCCCCCGCCCATCGGGCGCTccccc   >  2:3751881/1‑101 (MQ=255)
                                                                                                    |                                                                                                         
CCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCC  >  NC_014034/1397444‑1397644

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG