Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,552 C→G 13.6% L478L (CTC→GTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5520C→G13.6% 71.1 ‑2.1 0.0264L478L (CTC→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/20):  ref base (112/98):  total (132/132)
Fisher's exact test strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

GCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397452‑1397652
                                                                                                       |                                                                                                    
gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                      <  2:2175556/101‑1 (MQ=255)
 cGTGGATCGAAACACCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCa                                                                                                     <  2:4072528/101‑1 (MQ=255)
  gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                    >  1:2745492/1‑101 (MQ=255)
  gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAg                                                                                                    >  1:2976212/1‑101 (MQ=255)
   tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgc                                                                                                   <  1:139921/101‑1 (MQ=255)
   cgatctgAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgc                                                                                                   <  1:1847999/95‑1 (MQ=255)
    ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                                  >  2:4555664/1‑101 (MQ=255)
        cGAAAAACCGTCATCACCACGCAACCGCCCTGCACCACCGGCCGCTCCCCCCGCGGGGGCGGGGATCGAACCAACCCGACAATGCC.C..CCCCTAAGcgccgc                                                                                              >  2:2292805/1‑101 (MQ=37)
          aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCgcgc                                                                                            >  2:1902633/1‑101 (MQ=255)
           aaCCCCGTCATCACCACGCACCCGCCCTTCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCt                                                                                           <  1:1897870/101‑1 (MQ=255)
              acCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGAc                                                                                        <  1:2021081/101‑1 (MQ=255)
                cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGt                                                                                      >  1:1728192/1‑101 (MQ=255)
                 gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTc                                                                                     <  1:2844909/101‑1 (MQ=255)
                    atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                                  >  1:4259734/1‑101 (MQ=255)
                    atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCt                                                                                  <  1:3370832/101‑1 (MQ=255)
                       accccGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccc                                                                               <  2:601023/101‑1 (MQ=255)
                       accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGCCGTCGCTccc                                                                               >  2:1370096/1‑101 (MQ=255)
                        ccacGNNACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccc                                                                              <  1:2327019/101‑1 (MQ=255)
                         cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccccc                                                                             <  2:942914/101‑1 (MQ=255)
                          acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccccc                                                                            <  1:688969/101‑1 (MQ=255)
                           cGCGACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                           <  2:724879/101‑1 (MQ=255)
                           cGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                           <  1:653564/101‑1 (MQ=255)
                           cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCATGGCGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                           <  1:3986866/101‑1 (MQ=255)
                            gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                          >  2:1776701/1‑101 (MQ=255)
                            gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                          >  1:3735399/1‑101 (MQ=255)
                             cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg                                                                         >  1:2810384/1‑101 (MQ=255)
                              aaCCGCCCTGCGCCGCCGGTCCCACCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg                                                                        <  2:234324/101‑1 (MQ=38)
                              aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg                                                                        >  2:3868070/1‑101 (MQ=255)
                                 cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggggg                                                                     >  1:3172526/1‑101 (MQ=255)
                                  cccgtGCGCCACCGGTCGCCCCCCCGTCGGGGGCGTGGATCGAAACAGCCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCGCCCCCCGCGGGGGc                                                                    <  1:3548910/97‑1 (MQ=37)
                                    ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                                  <  1:3101167/101‑1 (MQ=255)
                                      tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg                                                                >  1:2096380/1‑101 (MQ=255)
                                      ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:1662507/91‑1 (MQ=2)
                                       gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  2:3517195/101‑1 (MQ=255)
                                        ccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  2:1592273/99‑1 (MQ=255)
                                         acaggatggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:3886349/92‑1 (MQ=2)
                                          cgttgcgTCGCTCCCCCCGCGGGGGCCTGGATCGAAACACCCCGAACCTGTCGA..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:774801/95‑1 (MQ=2)
                                          cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTC.G..GGGGCGAACACCGATACCGCGTCGCTCCCCCCGCGGGGCCGTGGATCg                                                            >  2:4619420/7‑101 (MQ=0)
                                          ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:4293502/101‑1 (MQ=255)
                                            accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  1:4600414/101‑1 (MQ=255)
                                              gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  1:2460562/99‑1 (MQ=2)
                                              cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  2:4221004/101‑1 (MQ=255)
                                              cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  2:116059/101‑1 (MQ=255)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                      <  1:3312653/101‑1 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                       >  2:1507559/1‑101 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                      <  1:4333897/101‑1 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggt                                                    <  1:2166338/101‑5 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                     <  1:3926598/101‑1 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                      <  1:1207155/101‑2 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                     <  1:602425/101‑1 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                   <  1:2699098/101‑6 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggc                                                   >  2:1030846/1‑96 (MQ=0)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGGGGATCGaaaaggggc                                                   >  1:884633/1‑95 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                    <  1:2927772/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                    <  2:1231429/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                    <  2:4330274/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                     >  1:3717322/1‑99 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                    >  2:2025660/1‑98 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                   >  2:2764952/1‑97 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                  >  2:280529/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtagatg                                                 >  2:1847543/1‑96 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                 >  2:3078909/1‑97 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                  >  1:3495139/1‑96 (MQ=0)
                                                     cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                <  2:3617962/100‑9 (MQ=2)
                                                     cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                 <  1:1947330/100‑5 (MQ=2)
                                                     cccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                 <  2:2928646/100‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                               <  1:72623/101‑10 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                 <  2:1967672/101‑7 (MQ=0)
                                                        ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                              <  1:2628110/101‑8 (MQ=2)
                                                        ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                              >  1:2610532/1‑94 (MQ=2)
                                                        ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                              <  2:2188897/101‑8 (MQ=2)
                                                         cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATcgatgatca                                             >  2:521830/1‑93 (MQ=0)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                             <  1:2766908/101‑1 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                           >  1:2494607/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                           >  2:3270514/1‑101 (MQ=255)
                                                            gcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                          <  2:4115329/101‑1 (MQ=255)
                                                              gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                        >  1:3048734/1‑101 (MQ=255)
                                                               ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                       <  1:3593312/101‑1 (MQ=255)
                                                                gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                      >  1:3407490/1‑101 (MQ=255)
                                                                 ggCGTGGATCGAAACACCCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                     <  1:1765857/101‑1 (MQ=255)
                                                                   cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                   <  2:3457423/101‑1 (MQ=255)
                                                                    gtcgATCAAAACACCCCGATCCTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                  <  2:4452179/98‑1 (MQ=38)
                                                                    gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCCGCGGATCGGCGTGAcccg                                  <  1:4143628/101‑1 (MQ=255)
                                                                       gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGTCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                               <  1:4000622/101‑1 (MQ=255)
                                                                        aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                              >  2:2085186/1‑101 (MQ=255)
                                                                        aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                              >  2:1095170/1‑101 (MQ=255)
                                                                           gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                           >  2:3677890/1‑101 (MQ=255)
                                                                               caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                       >  1:132750/1‑101 (MQ=255)
                                                                                aacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                      >  1:352202/1‑101 (MQ=255)
                                                                                  cccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt                    >  1:3823514/1‑101 (MQ=255)
                                                                                      agcATGTC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                <  2:1524283/99‑1 (MQ=255)
                                                                                      aTCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                <  1:4068725/101‑1 (MQ=255)
                                                                                      aTCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                >  2:2664275/1‑101 (MQ=255)
                                                                                         aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc             >  2:3983887/1‑101 (MQ=255)
                                                                                         aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc             <  2:2494441/101‑1 (MQ=255)
                                                                                            gC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc          >  2:2380990/1‑101 (MQ=255)
                                                                                               c..cGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc        <  2:3344601/101‑1 (MQ=255)
                                                                                                   gCCGCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg      <  1:1587090/101‑1 (MQ=255)
                                                                                                     cTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg    >  1:439575/1‑101 (MQ=255)
                                                                                                      tCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg   <  2:2613076/101‑1 (MQ=255)
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GCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397452‑1397652

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG