Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,560 C→A 16.3% A481E (GCG→GAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5600C→A16.3% 77.3 ‑1.7 ‑0.0252A481E (GCG→GAG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/20):  ref base (89/86):  total (123/129)
Fisher's exact test strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 287 total aligned reads displayed.

CGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397460‑1397660
                                                                                                       |                                                                                                    
      acCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGAc                                                                                                <  1:2021081/101‑1 (MQ=255)
        cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGt                                                                                              >  1:1728192/1‑101 (MQ=255)
             tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTc                                                                                         >  2:680126/1‑101 (MQ=255)
              caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcc                                                                                        >  1:1758253/1‑101 (MQ=255)
                ccacGNNACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccc                                                                                      <  1:2327019/101‑1 (MQ=255)
                 cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccccc                                                                                     <  2:942914/101‑1 (MQ=255)
                  acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccccc                                                                                    <  1:3502765/101‑1 (MQ=255)
                    gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                  >  2:2873118/1‑101 (MQ=255)
                     cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg                                                                                 >  2:4573429/1‑101 (MQ=255)
                      aaCCGCCCTGCGCCGCCGGTCCCACCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg                                                                                <  2:234324/101‑1 (MQ=38)
                          gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc                                                                            >  1:3362416/1‑101 (MQ=255)
                           cccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCg                                                                           <  2:3625684/101‑1 (MQ=255)
                            ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt                                                                          <  1:3101167/101‑1 (MQ=255)
                             cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                                         >  2:733346/1‑101 (MQ=255)
                              tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg                                                                        >  1:2096380/1‑101 (MQ=255)
                              ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  1:1662507/91‑1 (MQ=2)
                               gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa                                                                       <  2:3517195/101‑1 (MQ=255)
                                 atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:718833/8‑101 (MQ=0)
                                 acaggatggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:3886349/92‑1 (MQ=2)
                                  ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  2:4293502/101‑1 (MQ=255)
                                   caccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                   <  1:4545022/101‑1 (MQ=255)
                                    gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                                   >  1:786080/5‑101 (MQ=0)
                                     ccGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:3537631/101‑1 (MQ=255)
                                      cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  2:4221004/101‑1 (MQ=255)
                                      cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  2:116059/101‑1 (MQ=255)
                                       cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  1:3808232/100‑1 (MQ=2)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                              >  1:3719664/1‑101 (MQ=255)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggt                                                            <  1:2166338/101‑5 (MQ=2)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                              <  1:1207155/101‑2 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                           <  1:2699098/101‑6 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggc                                                           >  2:1030846/1‑96 (MQ=0)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                            <  2:4330274/101‑2 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                            <  1:2927772/101‑2 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGGGGATCGACACATcg                                                            >  2:2297310/1‑100 (MQ=2)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                             >  1:2747654/1‑99 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                            >  2:2025660/1‑98 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                            >  1:2784935/1‑98 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATccc                                                           >  2:1401004/1‑100 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                           <  1:1487832/101‑1 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                           <  2:1866339/101‑1 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                           >  2:2764952/1‑97 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGCGCA.CACCGATACCGCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACATcgatg                                                         >  1:3831201/1‑97 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                         >  2:3078909/1‑97 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                         >  1:410738/1‑97 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                          >  1:3495139/1‑96 (MQ=0)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                          >  1:3407149/1‑96 (MQ=0)
                                             cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                         <  1:1947330/100‑5 (MQ=2)
                                             cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                         <  2:529006/100‑5 (MQ=2)
                                             cccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                          <  1:2410790/100‑6 (MQ=0)
                                             cccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                         <  2:2928646/100‑1 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                         <  2:1967672/101‑7 (MQ=0)
                                              ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGAgg                                                        >  2:1813399/1‑101 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                       <  1:989310/101‑7 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGa                                                       >  2:1327648/1‑101 (MQ=255)
                                                ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATCCCGCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACATcgatgatc                                                      <  1:4617109/101‑8 (MQ=2)
                                                ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                      <  1:2628110/101‑8 (MQ=2)
                                                ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                      <  2:4043819/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                     <  1:3424577/101‑9 (MQ=0)
                                                 cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                     <  1:2833394/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                     <  2:4483735/101‑1 (MQ=255)
                                                 cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGACGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGagc                                                     >  2:4637007/1‑99 (MQ=255)
                                                   cggggggtCGTGGATCGAAACAACCCTATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                                   <  1:437389/98‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGNAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                                   <  2:1591307/101‑1 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                                   >  2:3270514/1‑101 (MQ=255)
                                                   cgcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                                   <  1:2486039/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                                  <  2:4115329/101‑1 (MQ=255)
                                                       ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                               >  2:152396/1‑101 (MQ=255)
                                                       ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                               <  1:3593312/101‑1 (MQ=255)
                                                        gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                              <  1:632945/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCACCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                             <  1:3994635/101‑1 (MQ=255)
                                                          gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                            <  1:2873334/101‑1 (MQ=255)
                                                           cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                           <  2:3457423/101‑1 (MQ=255)
                                                            gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGGGCCGCGCTGACGACGCTCCCCCCGCGGGTGCGTGGATCCAAACAACCGACGTTCTGCGGGAcccg                                          >  2:174330/1‑101 (MQ=255)
                                                            gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                          >  2:2517243/1‑101 (MQ=255)
                                                             tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGTGCCGAGCTGACGTCGCTGCCCCCGCTGGGGCGTGGATCGAAACATCCGCGGAACGGCGTGAccccc                                         >  2:3833732/1‑99 (MQ=38)
                                                             tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                         >  1:655630/1‑101 (MQ=255)
                                                                aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                      >  2:1095170/1‑101 (MQ=255)
                                                                aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                      >  2:2085186/1‑101 (MQ=255)
                                                                 tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCGTCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                     <  1:4005618/101‑1 (MQ=255)
                                                                       caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                               >  1:132750/1‑101 (MQ=255)
                                                                       caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                               >  1:4302263/1‑101 (MQ=255)
                                                                           ctgATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTc                           <  2:2188821/99‑1 (MQ=255)
                                                                           ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                           <  1:1843090/101‑1 (MQ=255)
                                                                           ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGACCCGCCCCGCCCCTCGGTc                           >  2:2227855/1‑101 (MQ=255)
                                                                           ccGACCAGGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                           <  1:425616/101‑1 (MQ=255)
                                                                            cGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                          <  1:2986720/101‑1 (MQ=255)
                                                                              aTCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                        >  2:2664275/1‑101 (MQ=255)
                                                                              aTCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                        <  2:3893709/101‑1 (MQ=255)
                                                                                    gC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                  >  2:2380990/1‑101 (MQ=255)
                                                                                       c..cGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc                <  2:3344601/101‑1 (MQ=255)
                                                                                          ccccTCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCcgcg               <  1:3283055/99‑1 (MQ=255)
                                                                                           gCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg              >  1:3970701/1‑101 (MQ=255)
                                                                                           gCCGCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg              <  1:1587090/101‑1 (MQ=255)
                                                                                              tCAGCGCCGCGCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg           <  2:2613076/101‑1 (MQ=255)
                                                                                                aGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg         >  1:2582337/1‑101 (MQ=255)
                                                                                                    ccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa     <  2:3970416/101‑1 (MQ=255)
                                                                                                     cgcgCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt    <  1:2534003/101‑1 (MQ=255)
                                                                                                     cgcgCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt    <  2:838341/101‑1 (MQ=255)
                                                                                                       cgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgggacc  >  1:3172003/1‑97 (MQ=255)
                                                                                                       cgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg  >  1:1893901/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
CGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1397460‑1397660

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG