Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,564 G→C 13.8% L482L (CTG→ACC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5640G→C13.8% 74.6 ‑2.1 ‑0.0263L482L (CTG→CTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/20):  ref base (114/99):  total (133/130)
Fisher's exact test strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.05e-01

Only 100 of 294 total aligned reads displayed.

ACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397464‑1397664
                                                                                                       |                                                                                                    
acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTg                                                                                                      >  2:3500112/1‑101 (MQ=255)
   ccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACg                                                                                                   <  1:2678088/101‑1 (MQ=255)
     gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTc                                                                                                 <  1:2844909/101‑1 (MQ=255)
         tcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTc                                                                                             >  2:680126/1‑101 (MQ=255)
          caccgcGCACCCGTCCTCCCCCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcc                                                                                            <  2:2744553/101‑1 (MQ=37)
           accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGCCGTCGCTccc                                                                                           >  2:1370096/1‑101 (MQ=255)
             cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccccc                                                                                         <  2:942914/101‑1 (MQ=255)
             cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTccccc                                                                                         <  2:3066163/101‑1 (MQ=255)
              acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccccc                                                                                        <  1:688969/101‑1 (MQ=255)
              acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTcccccc                                                                                        <  1:3502765/101‑1 (MQ=255)
               cGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                                                                                       <  1:653564/101‑1 (MQ=255)
                gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                      >  1:3735399/1‑101 (MQ=255)
                gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                                                                                      >  2:1776701/1‑101 (MQ=255)
                 cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg                                                                                     >  2:4573429/1‑101 (MQ=255)
                 cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgcg                                                                                     >  1:2810384/1‑101 (MQ=255)
                  aaCCGCCCTGCGCCGCCGGTCCCACCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg                                                                                    <  2:234324/101‑1 (MQ=38)
                  aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg                                                                                    <  1:2206861/101‑1 (MQ=255)
                     cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggggg                                                                                 <  2:3452186/101‑1 (MQ=255)
                         cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                                             >  1:1805772/1‑101 (MQ=255)
                         cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                                             >  2:733346/1‑101 (MQ=255)
                         cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg                                                                             <  1:2022339/101‑1 (MQ=255)
                            ccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          <  2:1592273/99‑1 (MQ=255)
                             atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          >  1:4271121/8‑101 (MQ=0)
                             acaggatggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  1:3886349/92‑1 (MQ=2)
                             acaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGCGGGTCg                                                                        >  2:2591913/8‑101 (MQ=0)
                              ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                        <  2:4293502/101‑1 (MQ=255)
                                accGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                      <  1:4600414/101‑1 (MQ=255)
                                 gatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAc                                                                    >  1:3143688/4‑101 (MQ=2)
                                 ccGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  1:3537631/101‑1 (MQ=255)
                                  cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                    <  2:4221004/101‑1 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                   >  2:1507559/1‑101 (MQ=2)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                  <  1:1962536/101‑1 (MQ=255)
                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                                  <  1:4333897/101‑1 (MQ=255)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                                  <  1:1207155/101‑2 (MQ=2)
                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                                 <  1:602425/101‑1 (MQ=255)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtc                                                               <  1:2744248/101‑6 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGTGGGTCGAAAcggggc                                                               >  1:3562191/1‑96 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCCCGGGGGCGGGGATCGaaaaggggc                                                               >  1:884633/1‑95 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                <  1:2927772/101‑2 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                <  2:1231429/101‑2 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                                <  2:4330274/101‑2 (MQ=2)
                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                                 >  1:3717322/1‑99 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                                >  2:2025660/1‑98 (MQ=2)
                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                               <  1:1487832/101‑1 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                              >  1:3344850/1‑101 (MQ=255)
                                        cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                                              >  2:280529/1‑101 (MQ=255)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtagatg                                                             >  2:1847543/1‑96 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                             >  1:410738/1‑97 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                             >  2:1088950/1‑97 (MQ=2)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                              >  1:3495139/1‑96 (MQ=0)
                                         tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                             >  1:2478826/1‑101 (MQ=255)
                                         cccccccGGGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                             <  2:3311328/100‑1 (MQ=255)
                                         cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                            <  2:3617962/100‑9 (MQ=2)
                                         cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                             <  2:529006/100‑5 (MQ=2)
                                         cccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                             <  2:2928646/100‑1 (MQ=255)
                                          ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCTATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                           <  1:72623/101‑10 (MQ=2)
                                          ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                             <  2:1967672/101‑7 (MQ=0)
                                            ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                          <  2:2188897/101‑8 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                                          >  1:1722554/1‑94 (MQ=2)
                                            ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                          >  1:2613261/1‑101 (MQ=255)
                                            ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                                          <  2:4043819/101‑1 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                         <  1:3424577/101‑9 (MQ=0)
                                             cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                         <  1:2766908/101‑1 (MQ=255)
                                             cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGACGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGagc                                                         >  2:4637007/1‑99 (MQ=255)
                                                gcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGc                                                      <  2:4115329/101‑1 (MQ=255)
                                                     ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCACCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                                 <  1:3994635/101‑1 (MQ=255)
                                                      gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                                <  2:2188378/101‑1 (MQ=255)
                                                        gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                              <  1:2132300/101‑1 (MQ=255)
                                                         tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGTGCCGAGCTGACGTCGCTGCCCCCGCTGGGGCGTGGATCGAAACATCCGCGGAACGGCGTGAccccc                                             >  2:3833732/1‑99 (MQ=38)
                                                           gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcgg                                           >  1:3381590/1‑94 (MQ=255)
                                                            aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                          >  2:2085186/1‑101 (MQ=255)
                                                            aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                          >  2:3249656/1‑101 (MQ=255)
                                                             tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCGTCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                         <  1:4005618/101‑1 (MQ=255)
                                                                   caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                                   >  1:132750/1‑101 (MQ=255)
                                                                      cccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGt                                >  1:3823514/1‑101 (MQ=255)
                                                                       ccGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                               <  2:102441/101‑1 (MQ=255)
                                                                        cGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                              <  1:2986720/101‑1 (MQ=255)
                                                                          agcATGTC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                            <  2:1524283/99‑1 (MQ=255)
                                                                          aTCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                            <  2:1125639/101‑1 (MQ=255)
                                                                             aTGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc                         <  2:2494441/101‑1 (MQ=255)
                                                                                gC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc                      >  2:2380990/1‑101 (MQ=255)
                                                                                       gCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg                  >  1:3970701/1‑101 (MQ=255)
                                                                                       gCCTCAGCGCCGCGCGGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg                  <  1:3227249/101‑1 (MQ=255)
                                                                                       gCCGCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgg                  <  1:1587090/101‑1 (MQ=255)
                                                                                         cTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg                >  1:439575/1‑101 (MQ=255)
                                                                                           cAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc              >  2:2630849/1‑101 (MQ=255)
                                                                                            aGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg             >  2:2239488/1‑101 (MQ=255)
                                                                                            aGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg             >  1:2582337/1‑101 (MQ=255)
                                                                                               gccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg          >  1:1746190/1‑101 (MQ=255)
                                                                                                ccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa         <  2:3970416/101‑1 (MQ=255)
                                                                                                ccgcGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa         >  1:2397332/1‑101 (MQ=255)
                                                                                                 cgcgCTGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt        <  2:3903600/101‑1 (MQ=255)
                                                                                                 cgcgCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt        <  1:2534003/101‑1 (MQ=255)
                                                                                                 cgcgCTGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt        <  2:838341/101‑1 (MQ=255)
                                                                                                   cgcTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg      >  1:1893901/1‑101 (MQ=255)
                                                                                                   cccTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  1:305815/99‑1 (MQ=255)
                                                                                                    gcTGACGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACATCCGCGGATCGGCGTGACCCGCCTCGCCCCTCGGTCGCTCCCCCCGCGGGGGAGGGGATCGa     >  2:4481218/1‑101 (MQ=38)
                                                                                                     cTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  2:3729443/101‑1 (MQ=255)
                                                                                                      tGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  1:1620517/1‑101 (MQ=255)
                                                                                                       gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  >  1:4251076/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
ACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397464‑1397664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG