BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,883	C→A	3.0%	intergenic (+187/+1711)	RCAP_rcc01307 → / ← RCAP_rcc01309	hypothetical protein/IS66 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,883	0	C→A	3.0%	9.0 ‑0.9 ‑0.1	202	intergenic (+187/+1711)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand): new base (4/2): ref base (88/108): total (92/110)
Fisher's exact test strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.71e-01

Only 100 of 237 total aligned reads displayed.


GGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCG  >  NC_014034/1397783‑1397983


 gagTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCcccg                                                                                                     <  2:2182148/99‑1 (MQ=255)


  cGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCcccgc                                                                                                    >  1:772150/1‑101 (MQ=255)


   gTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCcccgcc                                                                                                   >  2:2835845/1‑101 (MQ=255)


    tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCcccgccc                                                                                                  <  2:4095144/101‑1 (MQ=255)


     ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCcccgcccg                                                                                                 >  1:1731743/1‑101 (MQ=255)


       aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCt                                                                                               <  2:1919225/101‑1 (MQ=255)


        tCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTg                                                                                              >  2:2589450/1‑101 (MQ=255)


        tCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTg                                                                                              <  1:4489390/101‑1 (MQ=255)


          gAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGtc                                                                                            >  2:3839793/1‑101 (MQ=255)


           aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGtct                                                                                           >  2:2944336/1‑101 (MQ=255)


           aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAA..CTCAGCCCCGCCCGCTGtct                                                                                           >  2:1764428/1‑101 (MQ=255)


            aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGtctc                                                                                          <  2:3999184/101‑1 (MQ=255)


             aCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGtctcc                                                                                         >  2:1900609/1‑100 (MQ=255)


               ggTGTCCAGCGTGACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCAcc                                                                                       >  1:2532475/1‑101 (MQ=255)


               ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCCCTGTCTCAcc                                                                                       >  1:3665879/1‑101 (MQ=255)


                gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCa                                                                                      <  2:309017/101‑1 (MQ=255)


                gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCa                                                                                      <  2:191118/101‑1 (MQ=255)


                  gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                    >  2:155956/1‑101 (MQ=255)


                   tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                   <  1:4561146/101‑1 (MQ=255)


                   tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                   <  1:3840078/101‑1 (MQ=255)


                     cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                 <  2:38840/101‑1 (MQ=255)


                     cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                 <  1:3098531/101‑1 (MQ=255)


                      aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                >  1:4350476/1‑101 (MQ=255)


                         gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                             >  1:651283/1‑101 (MQ=255)


                         gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                             >  1:234946/1‑101 (MQ=255)


                          tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                            <  1:2123664/101‑1 (MQ=255)


                          tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                            <  2:496797/101‑1 (MQ=255)


                            acaTGCAGCGGCGTCGTATCGCCATGTCGCCCCCCCCGCGGGTGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                          <  2:203155/101‑1 (MQ=255)


                            acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                          >  1:2429417/1‑101 (MQ=255)


                            acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                          <  1:1056945/101‑1 (MQ=255)


                               tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                       >  2:4472639/1‑101 (MQ=255)


                               tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                       <  1:4066234/101‑1 (MQ=255)


                               tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                       <  2:760495/101‑1 (MQ=255)


                                  aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                                    >  2:4329540/1‑101 (MQ=255)


                                     ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                 >  1:3251769/1‑101 (MQ=255)


                                     ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                 >  1:2008577/1‑101 (MQ=255)


                                     ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                 <  2:317970/101‑1 (MQ=255)


                                     ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                 >  1:1592604/1‑101 (MQ=255)


                                       cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                               <  1:2913575/101‑1 (MQ=255)


                                         tCGCGTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGC.CCGGCAA..CTCAGCCCCGCCCGCTGGCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                             <  2:4544718/101‑1 (MQ=38)


                                          cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:2589635/101‑1 (MQ=255)


                                          cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:3781734/101‑1 (MQ=255)


                                          cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:181636/101‑1 (MQ=255)


                                          cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:46576/101‑1 (MQ=255)


                                          cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGAAC..CTCAGCCCCCCCCGCTGTCTCACCAACAGCCGCTCCCCCCGCGCGGGCggg                                                            >  1:3887715/1‑99 (MQ=37)


                                           gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:1134509/101‑1 (MQ=255)


                                            ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGC..CGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:3017994/92‑1 (MQ=0)


                                            cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:345847/101‑1 (MQ=255)


                                             aTCGCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:2969641/101‑1 (MQ=255)


                                              tccccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:103095/98‑1 (MQ=255)


                                              tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:1731624/101‑1 (MQ=255)


                                              tCGCCATGTCGCTCCCCCCGCGGGGGCGTCGATCAAACC.CCGGCAC..CTCAGCCCCCCCCCCTGTCGCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3665611/101‑1 (MQ=25)


                                               cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:1651787/101‑1 (MQ=255)


                                               cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  1:2262209/1‑101 (MQ=255)


                                                  ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACGTCTTGGAACAGCGCGCCAT.TGGCC.CCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  1:482172/4‑101 (MQ=2)


                                                  cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:357511/1‑101 (MQ=255)


                                                  cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:4240863/1‑101 (MQ=255)


                                                    tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                  <  1:2029159/101‑1 (MQ=255)


                                                    tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                  <  1:4291013/101‑1 (MQ=255)


                                                    tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                  >  1:4115292/1‑101 (MQ=255)


                                                    tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGGGGTcagatcg                                                  >  1:839660/1‑95 (MQ=37)


                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAGC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCCCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                 <  2:3562664/101‑1 (MQ=255)


                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                                 >  1:859420/1‑101 (MQ=255)


                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                                <  1:1878612/101‑1 (MQ=255)


                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                              <  2:143153/101‑1 (MQ=255)


                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                              >  1:3054244/1‑101 (MQ=255)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                                            <  1:983300/101‑1 (MQ=255)


                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCCAGGATCGAAACTCTATgg                                            >  2:785560/1‑101 (MQ=255)


                                                            cccccGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACTCTATggga                                          >  2:4193135/1‑101 (MQ=255)


                                                            cccccGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggga                                          <  1:2836061/101‑1 (MQ=255)


                                                             ccccGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                         <  2:4441557/101‑1 (MQ=255)


                                                              atctcgGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                                        >  2:2802117/5‑101 (MQ=255)


                                                                 gcgGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                                     >  2:4114109/1‑101 (MQ=255)


                                                                   gggggCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                   >  2:3688212/1‑101 (MQ=255)


                                                                     gggCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTTACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                                 >  1:3983640/1‑101 (MQ=255)


                                                                       gCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggagg                               >  2:234698/1‑101 (MQ=255)


                                                                        cGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggcggctagggaggc                              >  2:1153129/1‑101 (MQ=255)


                                                                        cGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                              <  1:2944565/101‑1 (MQ=255)


                                                                         gTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                             >  1:1325071/1‑101 (MQ=255)


                                                                          tGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                            >  2:67365/1‑101 (MQ=255)


                                                                          tGGATCGAAAC.CCGGCAC..CCCAGCCCCGCCCGCTCTCTCACCAACAGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                            <  2:859344/101‑1 (MQ=255)


                                                                           ggATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                           >  2:2285639/1‑101 (MQ=255)


                                                                            gATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                          <  1:3964668/101‑1 (MQ=255)


                                                                            gATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                          <  1:1475859/101‑1 (MQ=255)


                                                                             aTCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc                         >  1:520079/1‑101 (MQ=255)


                                                                               cGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                       >  2:1295068/1‑101 (MQ=255)


                                                                                 aaaC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                     <  1:4193423/101‑1 (MQ=255)


                                                                                  aaC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt                    <  1:2802333/101‑1 (MQ=255)


                                                                                   aC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc                   <  1:1905722/101‑1 (MQ=255)


                                                                                   aC.CCGGCAC..CTCAGCCCCGCCCGCTGTATCACCAACAGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtcc                   <  2:4081661/101‑3 (MQ=255)


                                                                                         gCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg              >  1:1536681/1‑101 (MQ=255)


                                                                                         gCAC..CTCAGCCCCGCCCGCTGGCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg              <  2:610396/101‑1 (MQ=255)


                                                                                          cAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt             <  2:1547175/101‑1 (MQ=255)


                                                                                               cTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGc          <  1:2774167/101‑1 (MQ=255)


                                                                                                 cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc        >  2:2425375/1‑101 (MQ=255)


                                                                                                     cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc    >  2:457957/1‑101 (MQ=255)


                                                                                                     cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc    <  1:720301/101‑1 (MQ=255)


                                                                                                      ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc   <  1:357548/101‑1 (MQ=255)


                                                                                                       cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg  <  2:3251524/101‑1 (MQ=255)


                                                                                                       cgcccgcTGTCTCACCAACAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg  <  1:4452357/101‑1 (MQ=255)


GGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCG  >  NC_014034/1397783‑1397983

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG