BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,890	T→A	1.8%	intergenic (+194/+1704)	RCAP_rcc01307 → / ← RCAP_rcc01309	hypothetical protein/IS66 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,890	0	T→A	1.8%	2.9 ‑1.1 ‑0.2	219	intergenic (+194/+1704)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand): new base (3/1): ref base (92/122): total (96/123)
Fisher's exact test strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.85e-01

Only 100 of 232 total aligned reads displayed.


ATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGG  >  NC_014034/1397790‑1397988


aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCTCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCt                                                                                                    >  2:3873893/1‑101 (MQ=255)


aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCt                                                                                                    <  2:4477909/101‑1 (MQ=255)


 tCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTg                                                                                                   >  2:2589450/1‑101 (MQ=255)


   gAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGtc                                                                                                 >  2:3839793/1‑101 (MQ=255)


    aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGtct                                                                                                >  2:2944336/1‑101 (MQ=255)


      aCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGtctcc                                                                                              >  2:1900609/1‑100 (MQ=255)


        ggTGTCCAGCGTGACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCAcc                                                                                            >  1:2532475/1‑101 (MQ=255)


           gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                         >  2:155956/1‑101 (MQ=255)


           gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                         <  1:174478/101‑1 (MQ=255)


            tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                        <  1:4561146/101‑1 (MQ=255)


              cAGCGCCACATGCAGCGGCGCCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                      <  2:4150632/101‑1 (MQ=255)


               aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                     >  1:4350476/1‑101 (MQ=255)


               aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                     <  1:77548/101‑1 (MQ=255)


                  gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                                  >  1:234946/1‑101 (MQ=255)


                  gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                                  <  2:3653525/101‑1 (MQ=255)


                     acaTGCAGCGGCGTCGTATCGCCATGTCGCCCCCCCCGCGGGTGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                               <  2:203155/101‑1 (MQ=255)


                     acaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                               <  1:1056945/101‑1 (MQ=255)


                       aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                             >  1:678395/1‑101 (MQ=255)


                       aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                             <  2:2301919/101‑1 (MQ=255)


                        tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                            <  1:4066234/101‑1 (MQ=255)


                         gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                           <  2:1863348/101‑1 (MQ=255)


                         gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                           <  1:3502049/101‑1 (MQ=255)


                              ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                      <  1:386495/101‑1 (MQ=255)


                              ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                      >  1:1195844/1‑101 (MQ=255)


                              ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                      <  2:317970/101‑1 (MQ=255)


                              ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                      >  1:2008577/1‑101 (MQ=255)


                              ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                      >  2:4303715/1‑101 (MQ=255)


                                cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                                    <  1:2913575/101‑1 (MQ=255)


                                  tCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                                  <  1:1551712/101‑1 (MQ=255)


                                   cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                 <  1:2589635/101‑1 (MQ=255)


                                   cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                 <  1:46576/101‑1 (MQ=255)


                                    gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                <  1:1134509/101‑1 (MQ=255)


                                     ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CACCAATGC..CGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                <  2:3017994/92‑1 (MQ=0)


                                     ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CACCAATGC..CGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                >  1:2126738/10‑101 (MQ=0)


                                      aTCGCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:2969641/101‑1 (MQ=255)


                                       tccccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:103095/98‑1 (MQ=255)


                                       tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:3766457/101‑1 (MQ=255)


                                       tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:1731624/101‑1 (MQ=255)


                                       cgatcttGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:839584/95‑1 (MQ=255)


                                        cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            >  1:2262209/1‑101 (MQ=255)


                                        cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            >  2:4550602/1‑101 (MQ=255)


                                           ggcctCGCCCCCCCCGCGGGGGCGTGGATCGAAC.C.CCGCCACGTCTTGGAACAGCGTGCTAT.TGGCC.CCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  2:1082909/97‑1 (MQ=2)


                                           cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:4240862/1‑101 (MQ=255)


                                           cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:357511/1‑101 (MQ=255)


                                            aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        >  2:3483505/1‑101 (MQ=255)


                                            aTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  1:2438615/101‑1 (MQ=255)


                                             tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                       <  1:2029159/101‑1 (MQ=255)


                                             tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                       >  2:1922316/1‑101 (MQ=255)


                                             tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                       >  1:4115292/1‑101 (MQ=255)


                                             tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGGGGTcagatcg                                                       >  1:839660/1‑95 (MQ=37)


                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAG.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCCCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                      <  2:3562664/101‑1 (MQ=255)


                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACC.CCAATGC..CGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                       >  1:1947895/1‑100 (MQ=2)


                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAActc                                                      >  1:859420/1‑101 (MQ=255)


                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                                     <  1:1878612/101‑1 (MQ=255)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                                   >  1:3054244/1‑101 (MQ=255)


                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                                   <  2:143153/101‑1 (MQ=255)


                                                     cccccGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggga                                               <  1:2836061/101‑1 (MQ=255)


                                                     cccccGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggga                                               <  2:678333/101‑1 (MQ=255)


                                                      ccccGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                              <  2:4441557/101‑1 (MQ=255)


                                                      ccccGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                              <  1:3793499/101‑1 (MQ=255)


                                                       atctcgGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggagg                                             >  2:2802117/5‑101 (MQ=255)


                                                         cgcgGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggct                                           >  2:471132/1‑101 (MQ=255)


                                                          gcgGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                                          >  2:4114109/1‑101 (MQ=255)


                                                           cgGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                         >  2:623629/1‑101 (MQ=255)


                                                            gggggCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                        >  2:3688212/1‑101 (MQ=255)


                                                              gggCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTTACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                                      >  1:3983640/1‑101 (MQ=255)


                                                              gggCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                                      <  2:2532301/101‑1 (MQ=255)


                                                                gCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggagg                                    <  1:3633921/101‑1 (MQ=255)


                                                                 cGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggcggctagggaggc                                   >  2:1153129/1‑101 (MQ=255)


                                                                 cGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                                   <  1:2944565/101‑1 (MQ=255)


                                                                 cGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                                   >  1:4278602/1‑101 (MQ=255)


                                                                  gTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                  <  2:3990572/101‑1 (MQ=255)


                                                                   tGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                 <  1:47978/101‑1 (MQ=255)


                                                                    ggATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                >  2:3045109/1‑101 (MQ=255)


                                                                     gATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                               >  2:4340832/1‑101 (MQ=255)


                                                                     gATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                               <  1:3964668/101‑1 (MQ=255)


                                                                     gATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                               <  1:1475859/101‑1 (MQ=255)


                                                                      aTCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc                              >  1:520079/1‑101 (MQ=255)


                                                                        cGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                            >  2:1295068/1‑101 (MQ=255)


                                                                         gAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                           >  2:899211/1‑101 (MQ=255)


                                                                          aaa.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                          <  1:4193423/101‑1 (MQ=255)


                                                                           aa.C.CCGGCAC..CTCAGCCCCGCCGGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt                         <  2:772075/101‑1 (MQ=255)


                                                                           aa.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt                         <  1:2802333/101‑1 (MQ=255)


                                                                            a.C.CCGGCAC..CTCAGCCCCGCCCGCTGTATCACCAACAGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtcc                        <  2:4081661/101‑3 (MQ=255)


                                                                                ccGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                      >  2:1917612/1‑101 (MQ=255)


                                                                                 cGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                     <  1:4475096/101‑1 (MQ=255)


                                                                                  ggCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                    <  2:234922/101‑1 (MQ=255)


                                                                                  ggCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                    <  2:4030613/101‑1 (MQ=255)


                                                                                   gCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg                   <  2:228437/101‑1 (MQ=255)


                                                                                   gCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg                   >  1:1536681/1‑101 (MQ=255)


                                                                                    cAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGGAGCTACGCACCTTCAAAAGt                  >  2:563080/1‑101 (MQ=255)


                                                                                    cAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                  <  2:1547175/101‑1 (MQ=255)


                                                                                         cTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGc               <  1:2774167/101‑1 (MQ=255)


                                                                                           cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc             <  2:1592499/101‑1 (MQ=255)


                                                                                           cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc             <  2:3323767/101‑1 (MQ=255)


                                                                                            aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcc            <  1:1827272/101‑1 (MQ=255)


                                                                                            aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcc            <  1:3102963/101‑1 (MQ=255)


                                                                                               cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc         <  1:623690/101‑1 (MQ=255)


                                                                                                ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc        <  2:3243515/101‑1 (MQ=255)


                                                                                                 cgcccgcTGTCTCACCAACAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg       <  1:4452357/101‑1 (MQ=255)


ATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.C.CCGGCAC..CTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGG  >  NC_014034/1397790‑1397988

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG