BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,434,387	C→A	11.6%	intergenic (‑166/‑792)	RCAP_rcc01334 ← / → RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,434,387	0	C→A	11.6%	80.0 ‑0.2 ‑0.1	278	intergenic (‑166/‑792)	RCAP_rcc01334/RCAP_rcc01335	hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand): new base (16/16): ref base (115/128): total (131/147)
Fisher's exact test strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.29e-01

Only 100 of 299 total aligned reads displayed.


TATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTC  >  NC_014034/1434287‑1434487


tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                       <  1:4329956/101‑1 (MQ=255)


     gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                  <  1:1199351/101‑1 (MQ=255)


     gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                  <  1:3787739/101‑1 (MQ=255)


     gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                  <  1:2936694/101‑1 (MQ=255)


      cACCGCTTACCGTGGATCGAAACAGGCTGCTTTCGGCCGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTg                                                                                                 <  1:3977901/101‑1 (MQ=255)


       aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                                >  2:916584/1‑101 (MQ=255)


       aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                                <  2:1680102/101‑1 (MQ=255)


        ccGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGTTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGCGGAGCGAAAA..GTCTCCGCTCTGCt                                                                                               >  1:1148141/1‑101 (MQ=39)


          gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                             >  1:524585/1‑101 (MQ=255)


           cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCa                                                                                            >  2:4457253/1‑101 (MQ=255)


             tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                          >  2:2143110/1‑101 (MQ=255)


             tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                          <  2:278140/101‑1 (MQ=255)


             tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GACTCCGCTCTGCTTCACg                                                                                          >  1:2604530/1‑101 (MQ=255)


               ccGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.Gc                                                                                       >  2:2965074/1‑101 (MQ=255)


                cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCCTCACG.GCt                                                                                      >  2:173798/1‑101 (MQ=255)


                 gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                     >  1:4385546/1‑101 (MQ=255)


                 gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                     >  2:3881484/1‑101 (MQ=255)


                 gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTg                                                                                     >  1:2507446/1‑101 (MQ=255)


                  tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGc                                                                                    >  2:1163816/1‑101 (MQ=255)


                      tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCa                                                                                >  2:492742/1‑101 (MQ=255)


                       cGAAACAGGCTGCTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCaa                                                                               <  1:1641680/101‑1 (MQ=255)


                           aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                           <  1:1415629/101‑1 (MQ=255)


                            cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTg                                                                          >  2:805140/1‑101 (MQ=255)


                             aGGCTGCTTTGGGCGGAACACCTTATCCTGCTGGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCCACCTTGt                                                                         >  2:2063153/1‑101 (MQ=255)


                              ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTc                                                                        >  1:3532554/1‑101 (MQ=255)


                                   ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcc                                                                   >  1:3052970/1‑101 (MQ=255)


                                   ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcc                                                                   >  1:937790/1‑101 (MQ=255)


                                   ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcc                                                                   <  1:1328441/101‑1 (MQ=255)


                                     ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccc                                                                 >  1:3495625/1‑101 (MQ=255)


                                      tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                <  2:4604041/101‑1 (MQ=255)


                                      tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                >  2:1313353/1‑101 (MQ=255)


                                       gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccccc                                                               >  1:2988419/1‑101 (MQ=255)


                                       gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccccc                                                               <  2:372250/101‑1 (MQ=255)


                                       gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTcccccc                                                               >  1:3272869/1‑101 (MQ=255)


                                         gCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcgc                                                             <  2:2600703/101‑1 (MQ=255)


                                          cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcgcg                                                            <  1:2770927/101‑1 (MQ=255)


                                           ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                           <  1:3511598/101‑1 (MQ=255)


                                              acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCggggg                                                        >  1:4214885/1‑101 (MQ=255)


                                                acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                      >  1:2038398/1‑101 (MQ=255)


                                                  cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                    <  1:2143260/101‑1 (MQ=255)


                                                   tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:4304572/101‑1 (MQ=255)


                                                    gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  1:3316604/1‑101 (MQ=255)


                                                    gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGGGGa                                                  >  2:328221/1‑101 (MQ=255)


                                                     aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 >  2:1414698/1‑101 (MQ=255)


                                                      tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:756763/101‑1 (MQ=255)


                                                      tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:1747232/101‑1 (MQ=255)


                                                      cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  1:1890770/92‑1 (MQ=2)


                                                       ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:565587/101‑1 (MQ=255)


                                                        ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  2:964127/101‑1 (MQ=255)


                                                        ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:674866/1‑101 (MQ=255)


                                                         tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  2:2178806/101‑1 (MQ=255)


                                                         tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:4177615/101‑1 (MQ=255)


                                                         gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                >  2:1647679/7‑101 (MQ=2)


                                                         gatctcgTCGCTCCCCCCGCGGGGGCGTTGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:1528104/95‑1 (MQ=2)


                                                           tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  1:3693654/5‑101 (MQ=2)


                                                            tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                          >  2:3926307/1‑101 (MQ=255)


                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  2:2326872/100‑1 (MQ=2)


                                                              cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                           <  1:1805111/100‑1 (MQ=2)


                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                          >  2:350379/1‑100 (MQ=2)


                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                      >  2:4005541/1‑101 (MQ=255)


                                                                  gccccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                    <  1:2874637/98‑1 (MQ=255)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                    <  2:4385254/101‑1 (MQ=255)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                    <  2:2828805/101‑1 (MQ=255)


                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                    >  1:2282561/1‑101 (MQ=255)


                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAAGGATTg                                   >  2:3547502/1‑101 (MQ=255)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                     >  2:183132/1‑95 (MQ=2)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                     >  2:3157763/1‑95 (MQ=2)


                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacactgct                                     <  2:1525978/101‑5 (MQ=2)


                                                                     ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                 >  2:62589/1‑101 (MQ=255)


                                                                      cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                   <  2:52579/101‑9 (MQ=2)


                                                                      cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                <  1:3040541/101‑1 (MQ=255)


                                                                       ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                               <  2:178708/101‑1 (MQ=255)


                                                                       ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                               >  2:942864/1‑101 (MQ=255)


                                                                         ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                             <  1:652041/101‑1 (MQ=255)


                                                                              ggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCgg                        >  2:1592784/1‑100 (MQ=255)


                                                                              ggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                        >  2:287308/1‑101 (MQ=255)


                                                                              ggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                        >  1:4517388/1‑101 (MQ=255)


                                                                               gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                       <  1:3764676/101‑1 (MQ=255)


                                                                               gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                       <  1:4414769/101‑1 (MQ=255)


                                                                                ggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                      <  1:4606876/101‑1 (MQ=255)


                                                                                 gCGTGGATCGAAAC..GTCTCCGCTCTGTTTCACG.GCTGCGTCAACCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                     <  1:3921921/101‑1 (MQ=255)


                                                                                  cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                    <  2:321228/101‑1 (MQ=255)


                                                                                     ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                 <  2:4153396/101‑1 (MQ=255)


                                                                                      gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCGGATTGCATGGCGCCGAGGGTCAgg                >  1:2802372/1‑101 (MQ=255)


                                                                                      gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGTGACAgg                <  2:3052726/101‑1 (MQ=255)


                                                                                       aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc               >  2:700890/1‑101 (MQ=255)


                                                                                       aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc               >  2:2729808/1‑101 (MQ=255)


                                                                                         cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt             >  2:2544789/1‑101 (MQ=255)


                                                                                          gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt            >  1:4104612/1‑101 (MQ=255)


                                                                                          gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt            <  1:3734791/101‑1 (MQ=255)


                                                                                          gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt            <  2:1759928/101‑1 (MQ=255)


                                                                                            aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc          <  1:2063293/101‑1 (MQ=255)


                                                                                              c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        <  1:3838794/101‑1 (MQ=255)


                                                                                              c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg        >  2:3594787/1‑101 (MQ=255)


                                                                                                  tctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc      <  1:1571422/101‑1 (MQ=255)


                                                                                                   ctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg     <  2:2510143/101‑1 (MQ=255)


                                                                                                    tcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc    >  1:2379243/1‑101 (MQ=255)


                                                                                                      cGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc  >  1:2188238/1‑101 (MQ=255)


                                                                                                      cGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc  <  1:1163908/101‑1 (MQ=255)


                                                                                                      cGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc  <  1:844708/101‑1 (MQ=255)


TATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTC  >  NC_014034/1434287‑1434487

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG