Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,390 T→C 12.7% intergenic (‑169/‑789) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3900T→C12.7% 82.3 ‑0.2 ‑0.5276intergenic (‑169/‑789)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (16/19):  ref base (115/125):  total (131/145)
Fisher's exact test strand distribution p-value = 8.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.09e-01

Only 100 of 295 total aligned reads displayed.

ATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCC  >  NC_014034/1434290‑1434490
                                                                                                       |                                                                                                       
atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ct                                                                                                         >  2:1175227/1‑101 (MQ=255)
atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ct                                                                                                         <  1:1970527/101‑1 (MQ=255)
  gCACCGCTTCCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctct                                                                                                       <  2:1823823/101‑1 (MQ=255)
  gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctct                                                                                                       <  2:883446/101‑1 (MQ=255)
  gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.ctct                                                                                                       <  1:2936694/101‑1 (MQ=255)
   cACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAC..GTCTCCG.CTCTg                                                                                                      <  2:2432069/101‑1 (MQ=255)
       gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTc                                                                                                 >  1:3521257/1‑101 (MQ=255)
        cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCa                                                                                                <  1:3557944/101‑1 (MQ=255)
         ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                               >  1:720066/1‑101 (MQ=255)
         ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCAc                                                                                               >  1:1669602/1‑101 (MQ=255)
              gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                         <  1:4201866/101‑1 (MQ=255)
              gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                         >  2:3881484/1‑101 (MQ=255)
                ggATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCTCTCCCCCCGCGGGGGCGTGGATCGCAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.ct                                                                                      >  1:4353143/1‑100 (MQ=255)
                 gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGt                                                                                     >  2:2874421/1‑101 (MQ=255)
                  aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTc                                                                                    <  2:63991/101‑1 (MQ=255)
                     gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                 <  1:2766325/101‑1 (MQ=255)
                     gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                 >  2:1313836/1‑101 (MQ=255)
                       aaCAGGCTGCTTTGGGCGGAACACCTGATCNTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                               <  1:1879391/101‑1 (MQ=255)
                       aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                               <  1:3696718/101‑1 (MQ=255)
                         cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTg                                                                             >  1:2178956/1‑101 (MQ=255)
                         cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTg                                                                             >  1:3772572/1‑101 (MQ=255)
                          aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGt                                                                            >  1:785918/1‑101 (MQ=255)
                              tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCGCCG.CTCCG.CTTCACG.GCTG.AGTCAATCTTGTCGCt                                                                        >  2:4437409/1‑101 (MQ=37)
                               gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTc                                                                       <  2:3196879/101‑1 (MQ=255)
                               gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTc                                                                       >  2:4606575/1‑101 (MQ=255)
                                ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcc                                                                      >  1:3052970/1‑101 (MQ=255)
                                 tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccc                                                                     <  1:2799537/101‑1 (MQ=255)
                                  ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccc                                                                    >  2:2159241/1‑101 (MQ=255)
                                  ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccc                                                                    >  1:3495625/1‑101 (MQ=255)
                                    gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                                  >  1:3272869/1‑101 (MQ=255)
                                    gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                                  >  1:2988419/1‑101 (MQ=255)
                                    gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                                  <  2:372250/101‑1 (MQ=255)
                                     ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCcg                                                                 <  1:536016/101‑1 (MQ=255)
                                          aaCCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                            <  1:1544539/101‑1 (MQ=255)
                                          aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                            >  1:2339473/1‑101 (MQ=255)
                                          aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                            >  1:178732/1‑101 (MQ=255)
                                           acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggggg                                                           >  1:4214885/1‑101 (MQ=255)
                                            cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                          <  2:2088441/101‑1 (MQ=255)
                                             acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                         <  1:90968/101‑1 (MQ=255)
                                              ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGt                                                        >  1:2098000/1‑101 (MQ=255)
                                               cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                       <  2:2748498/101‑1 (MQ=255)
                                                tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:4304572/101‑1 (MQ=255)
                                                 gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                     >  1:3316604/1‑101 (MQ=255)
                                                 gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTA.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                     >  1:3866924/1‑101 (MQ=255)
                                                  gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:3816331/91‑1 (MQ=2)
                                                  aTCCTGCTGGGTCGCACCCCCCGCGGGGGCGTGGCTCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:2219710/101‑1 (MQ=255)
                                                   cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:509264/92‑1 (MQ=2)
                                                    gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                     >  2:1761486/9‑101 (MQ=2)
                                                     gcctctcgTCGCCCCCACCGGGGGGGCGTCGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  2:3131461/94‑1 (MQ=0)
                                                     ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                 <  2:964127/101‑1 (MQ=255)
                                                      gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:971766/95‑1 (MQ=2)
                                                       gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:3762010/101‑1 (MQ=255)
                                                         tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                             >  2:3926307/1‑101 (MQ=255)
                                                         tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAaca                                             >  1:4488476/1‑101 (MQ=255)
                                                         atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  1:4110520/4‑101 (MQ=2)
                                                         atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                >  1:658813/4‑101 (MQ=2)
                                                          gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                            <  2:748633/101‑1 (MQ=255)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                             >  2:350379/1‑100 (MQ=2)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                             <  2:2791081/101‑2 (MQ=2)
                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTGCTGACACCGCCAACAAA.TATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                             <  1:115299/101‑2 (MQ=2)
                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                         >  2:4005541/1‑101 (MQ=255)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                           >  1:1262194/1‑98 (MQ=2)
                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                           >  1:206309/1‑98 (MQ=2)
                                                               gccccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                       <  1:2874637/98‑1 (MQ=255)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                          >  2:1993789/1‑97 (MQ=2)
                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTACG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                       <  1:3999806/101‑1 (MQ=255)
                                                                cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGATTg                                      >  1:1605890/1‑101 (MQ=255)
                                                                cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                      >  1:4652101/1‑101 (MQ=255)
                                                                cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAGTg                                      >  2:2210707/1‑101 (MQ=255)
                                                                 tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGGTTGc                                     >  2:547723/1‑101 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                   <  1:3040541/101‑1 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAAC..GACTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                   <  2:2604345/101‑1 (MQ=255)
                                                                    ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                  >  2:942864/1‑101 (MQ=255)
                                                                    ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                  <  1:4528654/101‑1 (MQ=255)
                                                                        gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                              >  1:4388995/1‑101 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                             <  1:1414793/101‑1 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                             >  2:2840235/1‑101 (MQ=255)
                                                                          gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                            <  1:3951109/101‑1 (MQ=255)
                                                                          gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                            <  2:3218284/101‑1 (MQ=255)
                                                                          gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                            <  1:629433/101‑1 (MQ=255)
                                                                           ggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCgg                           >  2:1592784/1‑100 (MQ=255)
                                                                             ggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAgg                         <  2:1661345/101‑1 (MQ=255)
                                                                                gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                      <  2:1732667/101‑1 (MQ=255)
                                                                                 tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                     <  2:917198/101‑1 (MQ=255)
                                                                                  ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                    <  2:1491105/101‑1 (MQ=255)
                                                                                  ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                    <  2:4153396/101‑1 (MQ=255)
                                                                                        aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg              <  1:2597101/101‑1 (MQ=255)
                                                                                        aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg              <  1:2805309/101‑1 (MQ=255)
                                                                                          aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa            <  2:2551529/101‑1 (MQ=255)
                                                                                          aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa            <  2:2527067/101‑1 (MQ=255)
                                                                                          aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa            <  2:4492058/101‑1 (MQ=255)
                                                                                           c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg           >  2:3594787/1‑101 (MQ=255)
                                                                                           c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg           <  2:1741810/101‑1 (MQ=255)
                                                                                              gTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt          <  1:2385628/101‑1 (MQ=255)
                                                                                              gTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt          <  1:1197904/101‑1 (MQ=255)
                                                                                               tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc         >  1:1486492/1‑101 (MQ=255)
                                                                                                 tcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc       <  2:3532296/101‑1 (MQ=255)
                                                                                                 tcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc       <  2:3043609/101‑1 (MQ=255)
                                                                                                      ctctG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGGCAGGCTTTGCAGTCGCTccc   >  2:1635489/1‑101 (MQ=255)
                                                                                                       tctG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc  <  1:1093017/101‑1 (MQ=255)
                                                                                                       |                                                                                                       
ATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCC  >  NC_014034/1434290‑1434490

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG