Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,406 G→A 12.3% intergenic (‑185/‑773) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4060G→A12.3% 84.2 ‑0.2 ‑0.0274intergenic (‑185/‑773)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (16/18):  ref base (108/132):  total (124/150)
Fisher's exact test strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.75e-01

Only 100 of 283 total aligned reads displayed.

GATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1434307‑1434506
                                                                                                         |                                                                                                      
 aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCCCG.GCTG.CGTc                                                                                                      >  1:2302557/1‑101 (MQ=255)
 aTCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.Cgcc                                                                                                      >  1:3279339/1‑99 (MQ=255)
  tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCa                                                                                                     >  2:492742/1‑101 (MQ=255)
      aaCAGGCTGCTTTGGGCGGAACACCTGATCNTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCt                                                                                                 <  1:1879391/101‑1 (MQ=255)
      aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTTTG.CTTCACG.GCTG.CGTCAACCt                                                                                                 <  2:3520964/101‑1 (MQ=255)
       aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                                                <  1:1415629/101‑1 (MQ=255)
        cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..g                                                                                             >  2:805140/1‑101 (MQ=255)
        cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..g                                                                                             >  1:2178956/1‑101 (MQ=255)
         aGGCTGCTTTGGGCGGAACACCTTATCCTGCTGGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCCACCTT..Gt                                                                                            >  2:2063153/1‑101 (MQ=255)
         aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..Gt                                                                                            >  2:2626646/1‑101 (MQ=255)
         aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..Gt                                                                                            >  2:2804979/1‑101 (MQ=255)
         aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..Gt                                                                                            >  2:162380/1‑101 (MQ=255)
         aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..Gt                                                                                            >  1:785918/1‑101 (MQ=255)
          ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTc                                                                                           <  2:3553959/101‑1 (MQ=255)
               ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcc                                                                                      >  1:937790/1‑101 (MQ=255)
               ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcc                                                                                      <  1:1328441/101‑1 (MQ=255)
               ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcc                                                                                      >  1:3052970/1‑101 (MQ=255)
                 ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccc                                                                                    >  1:3495625/1‑101 (MQ=255)
                 ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccc                                                                                    >  2:2159241/1‑101 (MQ=255)
                  tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                                   >  2:1313353/1‑101 (MQ=255)
                  tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                                   <  2:4604041/101‑1 (MQ=255)
                   gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                                  <  2:372250/101‑1 (MQ=255)
                   gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                                  >  1:2988419/1‑101 (MQ=255)
                    ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcg                                                                                 <  2:672895/101‑1 (MQ=255)
                    ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcg                                                                                 <  1:536016/101‑1 (MQ=255)
                     gCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgc                                                                                <  2:2600703/101‑1 (MQ=255)
                      cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgcg                                                                               <  1:2770927/101‑1 (MQ=255)
                       ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgg                                                                              <  1:3511598/101‑1 (MQ=255)
                         aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                            >  1:178732/1‑101 (MQ=255)
                            acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                                                         >  1:2038398/1‑101 (MQ=255)
                             ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                        >  1:2098000/1‑101 (MQ=255)
                               tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                      >  2:2870376/1‑101 (MQ=255)
                               tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                                      <  2:4445119/101‑1 (MQ=255)
                                gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:3316604/1‑101 (MQ=255)
                                gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGGGGa                                                                     >  2:328221/1‑101 (MQ=255)
                                gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTA.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGa                                                                     >  1:3866924/1‑101 (MQ=255)
                                 gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTg                                                                       <  2:1650218/91‑1 (MQ=2)
                                 gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTg                                                                       <  1:3816331/91‑1 (MQ=2)
                                    ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  1:674866/1‑101 (MQ=255)
                                    ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 <  2:964127/101‑1 (MQ=255)
                                       tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                 >  1:3693654/5‑101 (MQ=2)
                                        tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                             >  2:3106455/1‑101 (MQ=255)
                                        tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                             >  2:3926307/1‑101 (MQ=255)
                                        atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                >  1:4110520/4‑101 (MQ=2)
                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTGCTGACACCGCCAACAAA.TATGTGGTCAACACT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                             <  1:115299/101‑2 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                         >  2:4005541/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                           >  1:206309/1‑98 (MQ=2)
                                              gccccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                       <  1:2874637/98‑1 (MQ=255)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                          >  2:1993789/1‑97 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACGGATTg                                                      >  1:165096/1‑101 (MQ=255)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAGTg                                                      >  2:2210707/1‑101 (MQ=255)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                        >  2:3157763/1‑95 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                        >  1:2205732/1‑95 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTACG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                     >  1:4204088/1‑101 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                    >  2:62589/1‑101 (MQ=255)
                                                  cccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                                      <  2:52579/101‑9 (MQ=2)
                                                   ccccGCGGGGGCGTGGATCGAAAC..TTGTCTG.ACGCG.CTCATCA.GC.A.CGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                   <  2:2763772/101‑8 (MQ=37)
                                                   ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                  <  2:178708/101‑1 (MQ=255)
                                                   ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                  >  2:942864/1‑101 (MQ=255)
                                                     ccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                <  1:652041/101‑1 (MQ=255)
                                                       gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                              >  1:4388995/1‑101 (MQ=255)
                                                        cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                             >  1:3007899/1‑101 (MQ=255)
                                                          ggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                                           >  1:4517388/1‑101 (MQ=255)
                                                           gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                          <  1:3764676/101‑1 (MQ=255)
                                                           gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                          <  1:4414769/101‑1 (MQ=255)
                                                             gCGTGGATCGAAAC..GTCTCCG.CTCTG.TTTCACG.GCTG.CGTCAACCGT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                        <  1:3921921/101‑1 (MQ=255)
                                                              cGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                       >  2:3786169/1‑101 (MQ=255)
                                                              cGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                       <  2:321228/101‑1 (MQ=255)
                                                               gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                      <  2:1732667/101‑1 (MQ=255)
                                                               gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                      <  2:422985/101‑1 (MQ=255)
                                                               gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                      <  2:2503162/101‑1 (MQ=255)
                                                                 ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                    <  2:1491105/101‑1 (MQ=255)
                                                                   aTCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                                  >  1:4245053/1‑101 (MQ=255)
                                                                    tCGAATC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCGGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt                                 <  2:4352851/101‑1 (MQ=255)
                                                                    tCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt                                 <  1:1582644/101‑1 (MQ=255)
                                                                     cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                >  2:2969037/1‑101 (MQ=255)
                                                                     cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                >  2:2544789/1‑101 (MQ=255)
                                                                      gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                               <  1:4567654/101‑1 (MQ=255)
                                                                      gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                               <  2:1759928/101‑1 (MQ=255)
                                                                      gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                               <  1:3734791/101‑1 (MQ=255)
                                                                       aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                              <  1:2805309/101‑1 (MQ=255)
                                                                       aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                              <  1:2597101/101‑1 (MQ=255)
                                                                        aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                             <  1:1540416/101‑1 (MQ=255)
                                                                          c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                           <  1:98848/101‑1 (MQ=255)
                                                                             gTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                          <  1:1197904/101‑1 (MQ=255)
                                                                             gTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                          <  1:2385628/101‑1 (MQ=255)
                                                                              tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                         >  1:1486492/1‑101 (MQ=255)
                                                                                tcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc                       >  1:2379243/1‑101 (MQ=255)
                                                                                     ctctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                   >  2:924117/1‑101 (MQ=255)
                                                                                       ctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                 <  2:1586371/101‑1 (MQ=255)
                                                                                         g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGGAGTCGCTgcccccc               >  2:3437585/1‑100 (MQ=255)
                                                                                         g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg               <  1:487480/101‑1 (MQ=255)
                                                                                           cTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc              >  1:899433/1‑101 (MQ=255)
                                                                                             tCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg            >  2:141347/1‑101 (MQ=255)
                                                                                             tCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg            >  1:1033672/1‑101 (MQ=255)
                                                                                              cACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggg           <  1:2965303/101‑1 (MQ=255)
                                                                                               aCG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGCGGGACCGGCTTTGCAGTCGCTCCCCCCGCgggg          >  1:4023038/1‑101 (MQ=255)
                                                                                               aCG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggg          <  1:578051/101‑1 (MQ=255)
                                                                                                cG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggggg         <  2:1638545/101‑1 (MQ=255)
                                                                                                    cTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGt      <  2:364324/101‑1 (MQ=39)
                                                                                                         |                                                                                                      
GATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGAT  >  NC_014034/1434307‑1434506

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG