Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,413 T→G 12.6% intergenic (‑192/‑766) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4130T→G12.6% 82.5 0.0 ‑0.0274intergenic (‑192/‑766)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (16/19):  ref base (107/131):  total (123/151)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.79e-01

Only 100 of 283 total aligned reads displayed.

AACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434313‑1434510
                                                                                                          |                                                                                                 
 aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCtt                                                                                                  <  1:1415629/101‑1 (MQ=255)
  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTg                                                                                                 >  2:805140/1‑101 (MQ=255)
  cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTg                                                                                                 >  1:2178956/1‑101 (MQ=255)
   aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGt                                                                                                >  1:785918/1‑101 (MQ=255)
    ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTc                                                                                               <  2:3553959/101‑1 (MQ=255)
    ggCTGCTTTGGGCGAAACACCTGATCCTGCTTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GGCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTc                                                                                               <  1:2383761/101‑1 (MQ=255)
       tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCGCCG.CTCCG.CTTCACG.GCTG.AGTCAATCTTGTCGCt                                                                                            >  2:4437409/1‑101 (MQ=37)
         ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcc                                                                                          <  1:1328441/101‑1 (MQ=255)
         ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcc                                                                                          >  1:937790/1‑101 (MQ=255)
          tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccc                                                                                         <  1:2799537/101‑1 (MQ=255)
           ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccc                                                                                        >  1:3495625/1‑101 (MQ=255)
            tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTccccc                                                                                       <  2:1768411/101‑1 (MQ=255)
             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                                                      >  1:3272869/1‑101 (MQ=255)
             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                                                      <  2:372250/101‑1 (MQ=255)
             gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTcccccc                                                                                      >  1:2988419/1‑101 (MQ=255)
              ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCcg                                                                                     <  2:672895/101‑1 (MQ=255)
              ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCcg                                                                                     <  1:536016/101‑1 (MQ=255)
                   aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                                                >  2:1743194/1‑101 (MQ=255)
                   aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCgggg                                                                                >  2:4567355/1‑101 (MQ=255)
                    acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCggggg                                                                               >  1:4214885/1‑101 (MQ=255)
                     cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                                              <  2:2088441/101‑1 (MQ=255)
                      acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                                             >  1:2038398/1‑101 (MQ=255)
                       ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGt                                                                            >  1:2098000/1‑101 (MQ=255)
                        cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                                           >  2:522570/1‑101 (MQ=255)
                         tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:4304572/101‑1 (MQ=255)
                          gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  1:3316604/1‑101 (MQ=255)
                          gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTA.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         >  1:3866924/1‑101 (MQ=255)
                           gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:3816331/91‑1 (MQ=2)
                            tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:1747232/101‑1 (MQ=255)
                            ggggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  2:2163742/92‑1 (MQ=2)
                            cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1890770/92‑1 (MQ=2)
                             ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGTGCGTGGATCg                                                                      <  2:1561635/101‑1 (MQ=255)
                              ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:3047335/8‑101 (MQ=2)
                              gcctctcgTCGCCCCCACCGGGGGGGCGTCGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        <  2:3131461/94‑1 (MQ=0)
                              ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  1:674866/1‑101 (MQ=255)
                              ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  2:964127/101‑1 (MQ=255)
                               tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  1:4177615/101‑1 (MQ=255)
                               gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  2:1647679/7‑101 (MQ=2)
                                 tatcgTCGCTCCTCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  1:2527232/5‑101 (MQ=2)
                                 tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  1:3693654/5‑101 (MQ=2)
                                 ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:3526741/101‑1 (MQ=255)
                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  2:3926307/1‑101 (MQ=255)
                                  tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  1:2829021/1‑101 (MQ=255)
                                  atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                                    >  1:658813/4‑101 (MQ=2)
                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:1805111/100‑1 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                                 >  2:350379/1‑100 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                                 <  2:1711902/101‑2 (MQ=2)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTGCTGACACCGCCAACAAA.TATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                                 <  1:115299/101‑2 (MQ=2)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                                             >  2:4005541/1‑101 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgct                                                               >  1:206309/1‑98 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACACGGAt                                                            >  2:1609919/1‑101 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAtt                                                           <  2:2828805/101‑1 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            >  2:3169582/1‑95 (MQ=2)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            >  1:2205732/1‑95 (MQ=2)
                                            cccccGCGGGGGCGTGGATCGAAAC..GACTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                                       <  2:2604345/101‑1 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                      >  2:942864/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                <  2:3218284/101‑1 (MQ=255)
                                                   gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                <  1:3951109/101‑1 (MQ=255)
                                                    ggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                                               >  1:4517388/1‑101 (MQ=255)
                                                    ggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                                               >  2:287308/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              <  1:4414769/101‑1 (MQ=255)
                                                     gggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              <  1:3764676/101‑1 (MQ=255)
                                                       gCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                            <  2:556263/101‑1 (MQ=255)
                                                        cGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                           >  2:3786169/1‑101 (MQ=255)
                                                           ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        <  2:1491105/101‑1 (MQ=255)
                                                             aTCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                                      >  2:2729808/1‑101 (MQ=255)
                                                              tCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt                                     <  1:1582644/101‑1 (MQ=255)
                                                                gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  1:4104612/1‑101 (MQ=255)
                                                                gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:3734791/101‑1 (MQ=255)
                                                                gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:4567654/101‑1 (MQ=255)
                                                                gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  1:2926231/101‑1 (MQ=255)
                                                                  aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  1:1540416/101‑1 (MQ=255)
                                                                   aC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                <  2:2551529/101‑1 (MQ=255)
                                                                       gTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              <  1:1197904/101‑1 (MQ=255)
                                                                        tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                             >  1:1486492/1‑101 (MQ=255)
                                                                         ctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                            <  2:2510143/101‑1 (MQ=255)
                                                                          tcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc                           <  2:3043609/101‑1 (MQ=255)
                                                                            cG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                         >  2:2272099/1‑101 (MQ=255)
                                                                            cG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAATCGCTc                         >  1:4369718/1‑101 (MQ=255)
                                                                               ctctG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                       >  2:924117/1‑101 (MQ=255)
                                                                                   g.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  1:487480/101‑1 (MQ=255)
                                                                                   g.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   >  2:1248346/1‑101 (MQ=255)
                                                                                     cTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc                  >  1:899433/1‑101 (MQ=255)
                                                                                      ttCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 <  2:1958763/101‑1 (MQ=255)
                                                                                       tCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  1:1033672/1‑101 (MQ=255)
                                                                                       tCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg                >  2:141347/1‑101 (MQ=255)
                                                                                         aCG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggg              <  1:578051/101‑1 (MQ=255)
                                                                                          cG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggggg             <  2:1638545/101‑1 (MQ=255)
                                                                                              cTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGt          <  2:364324/101‑1 (MQ=39)
                                                                                                  cGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGa       <  2:2784417/101‑1 (MQ=35)
                                                                                                  cGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCCCGGGGGGGTggc       >  1:3006716/1‑100 (MQ=255)
                                                                                                   gTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGAt      <  1:2312924/101‑1 (MQ=34)
                                                                                                   gTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGAt      >  2:100972/1‑101 (MQ=34)
                                                                                                    tCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:4203800/101‑1 (MQ=33)
                                                                                                    tCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:2052371/101‑1 (MQ=33)
                                                                                                      aaCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGTAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:4005826/101‑1 (MQ=14)
                                                                                                      aaCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   <  1:2626831/101‑1 (MQ=31)
                                                                                                      aaCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:3526723/1‑101 (MQ=31)
                                                                                                       aCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:4669171/101‑1 (MQ=11)
                                                                                                       aCCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:3316355/101‑1 (MQ=11)
                                                                                                          |                                                                                                 
AACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434313‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG