Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,443,778 G→C 100% A33A (GCC→GCG RCAP_rcc01340 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,443,7780G→C100.0% 738.4 198A33A (GCC→GCGRCAP_rcc01340LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (103/94):  ref base (1/0):  total (104/94)

Only 100 of 213 total aligned reads displayed.

GCCGCGCCCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGGTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCA  >  NC_014034/1443680‑1443875
                                                                                                  |                                                                                                 
gccgcgccCTCGTCGGACAGTTCGTGCCCGGTCTGGCGGAGCAGGAACAGCGGCAGGCCAAGCGTCTCCTTCATCCGCTCGATGCGCCGCGAGAACGTGGc                                                                                                 >  2:3696916/1‑101 (MQ=255)
  cgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCaa                                                                                               <  2:4246348/101‑1 (MQ=255)
  cgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCaa                                                                                               <  1:3274427/101‑1 (MQ=255)
  cgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCaa                                                                                               >  2:3545987/1‑101 (MQ=255)
   gcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAAc                                                                                              <  2:3298948/101‑1 (MQ=255)
   gcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAAc                                                                                              <  2:1048343/101‑1 (MQ=255)
   gcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAAc                                                                                              <  1:4371568/101‑1 (MQ=255)
    cgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACg                                                                                             >  1:3729016/1‑101 (MQ=255)
       ccTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCa                                                                                          <  2:2331589/101‑1 (MQ=255)
        cTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCCATGCGCCGCGAGATCGTCGCAACGCCaa                                                                                         >  1:212417/1‑101 (MQ=255)
              ggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCa                                                                                   >  2:3371652/1‑101 (MQ=255)
              ggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCa                                                                                   <  1:3678919/101‑1 (MQ=255)
               gtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAg                                                                                  <  1:2869707/101‑1 (MQ=255)
               gtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAg                                                                                  >  2:617974/1‑101 (MQ=255)
                tcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAgc                                                                                 >  1:1191875/1‑100 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                >  1:50391/1‑101 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                >  2:1446906/1‑101 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                <  1:3052075/101‑1 (MQ=255)
                   gTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGACGAGCGTCGCCTCCATCCGCCCGATGCGCCGCGCGATCGTCGCCACGCCAAGCACCAGTctc                                                                              >  2:3204791/1‑99 (MQ=255)
                    ttCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgcc                                                                             <  2:1227754/101‑1 (MQ=255)
                    ttCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgcc                                                                             >  1:2805529/1‑101 (MQ=255)
                      cGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgc                                                                           >  1:839541/1‑101 (MQ=255)
                      cGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgc                                                                           >  1:1015310/1‑101 (MQ=255)
                       gTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTccccgcc                                                                          >  2:696617/1‑100 (MQ=255)
                          cccGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgcgccg                                                                       >  1:3009217/1‑101 (MQ=255)
                             ggTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCcgcgccgcgc                                                                    >  1:2596138/1‑101 (MQ=255)
                              gTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGCCCCAGTCGCCgcgccgcgcc                                                                   >  2:1130847/1‑101 (MQ=255)
                                cTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCt                                                                 <  2:3968232/101‑1 (MQ=255)
                                cTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCt                                                                 <  1:4665699/101‑1 (MQ=255)
                                  ggcggcGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGc                                                               >  2:445597/1‑101 (MQ=255)
                                  ggcggcGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGc                                                               >  1:1176576/1‑101 (MQ=255)
                                   gcggcgCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCa                                                              <  2:2135289/101‑1 (MQ=255)
                                   gcggcgCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCa                                                              <  1:1856779/101‑1 (MQ=255)
                                     ggcgCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGc                                                            <  2:4150330/101‑1 (MQ=255)
                                      gcgcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCg                                                           <  1:3968804/101‑1 (MQ=255)
                                      gcgcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCg                                                           <  2:2127763/101‑1 (MQ=255)
                                       cgcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGt                                                          >  2:3194518/1‑101 (MQ=255)
                                       cgcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGt                                                          >  2:1772445/1‑101 (MQ=255)
                                         cAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGc                                                        >  1:1684187/1‑101 (MQ=255)
                                          aGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGcc                                                       >  1:4494438/1‑101 (MQ=255)
                                           ggAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGccc                                                      <  1:1313360/101‑1 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     <  2:1516751/101‑1 (MQ=255)
                                             aaCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTc                                                    <  1:4196608/101‑1 (MQ=255)
                                             aaCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTc                                                    >  2:3804621/1‑101 (MQ=255)
                                                aGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcg                                                 <  1:4450733/101‑1 (MQ=255)
                                                aGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcg                                                 <  2:2872247/101‑1 (MQ=255)
                                                 gCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcgc                                                <  2:190336/101‑1 (MQ=255)
                                                     cAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcca                                            <  2:347836/101‑1 (MQ=255)
                                                      aGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccag                                           >  2:4181434/1‑101 (MQ=255)
                                                      aGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccag                                           >  1:1502797/1‑101 (MQ=255)
                                                       ggCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCCTGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagc                                          >  1:3989994/1‑101 (MQ=255)
                                                         ccGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgc                                        >  2:2062289/1‑101 (MQ=255)
                                                         ccGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgc                                        >  2:1804739/1‑101 (MQ=255)
                                                          cGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcc                                       <  2:2802126/101‑1 (MQ=255)
                                                           gAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcca                                      <  2:814268/101‑1 (MQ=255)
                                                           gAGCGTCGCATCCATCCCCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcca                                      >  2:3769554/1‑101 (MQ=255)
                                                            aGCGTCGCCTCCATGCGCACGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcaccagcgccag                                     <  2:3123844/101‑1 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     >  1:1217736/1‑101 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     >  1:4439355/1‑101 (MQ=255)
                                                             gCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGa                                    >  2:1943618/1‑101 (MQ=255)
                                                              cGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGaa                                   >  1:2971064/1‑101 (MQ=255)
                                                               gTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGaaa                                  >  1:984349/1‑101 (MQ=255)
                                                                tCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAAc                                 <  2:3989226/101‑1 (MQ=255)
                                                                tCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAAc                                 <  1:4066967/101‑1 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                <  1:1772564/101‑1 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                <  1:4576355/101‑1 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                >  1:1207070/1‑101 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                <  2:3906714/101‑1 (MQ=255)
                                                                 cGCCTCCACCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                <  2:16410/101‑1 (MQ=255)
                                                                  gCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAg                               <  1:3746870/101‑1 (MQ=255)
                                                                   ccTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGc                              <  1:2434894/101‑1 (MQ=255)
                                                                     tCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCg                            >  2:1263497/1‑101 (MQ=255)
                                                                     tCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCg                            >  2:2453375/1‑101 (MQ=255)
                                                                     tCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCg                            >  2:2491335/1‑101 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         >  1:2708090/1‑101 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         >  1:3593861/1‑101 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         >  1:4095580/1‑101 (MQ=255)
                                                                          ccGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgt                       <  2:3618629/101‑1 (MQ=255)
                                                                           cgctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtc                      <  2:1168170/98‑1 (MQ=255)
                                                                           cgctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtc                      >  1:627921/4‑101 (MQ=255)
                                                                            gctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtcg                     <  2:845927/99‑1 (MQ=255)
                                                                              tCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtcgtc                   >  1:132636/1‑101 (MQ=255)
                                                                                gATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 >  1:2254220/1‑101 (MQ=255)
                                                                                   gcgcCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGACAGAAACAGCCGGATGTCGTCCCAGt              >  2:767167/1‑101 (MQ=255)
                                                                                      ccgcGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCt           <  1:2605618/101‑1 (MQ=255)
                                                                                      ccgcGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCt           <  1:1998016/101‑1 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         <  2:50385/101‑1 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         <  1:1908793/101‑1 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         <  2:3355389/101‑1 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         <  2:3145984/101‑1 (MQ=255)
                                                                                         ggagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgtg        <  2:2694216/100‑1 (MQ=255)
                                                                                         cgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgtg        <  2:2595953/101‑1 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCTCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       >  1:1358002/1‑101 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       >  1:344935/1‑101 (MQ=255)
                                                                                           agaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGc      >  1:4351809/1‑101 (MQ=255)
                                                                                           agaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGc      >  1:1439987/1‑101 (MQ=255)
                                                                                            gaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCt     >  1:1624859/1‑101 (MQ=255)
                                                                                             aTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTc    <  1:3371902/101‑1 (MQ=255)
                                                                                             aTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCGGTCGCTc    <  2:3730388/101‑1 (MQ=255)
                                                                                              tCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTcc   <  2:2627172/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCCGCGCCCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGGTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCA  >  NC_014034/1443680‑1443875

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG