Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,446,527 C→T 13.0% A142A (GCC→GCT RCAP_rcc01343 → iron siderophore/cobalamin ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,446,5270C→T13.0% 59.3 ‑0.7 ‑0.0177A142A (GCC→GCTRCAP_rcc01343iron siderophore/cobalamin ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (10/13):  ref base (79/75):  total (89/88)
Fisher's exact test strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.73e-01

Only 100 of 217 total aligned reads displayed.

TCTCGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTGA  >  NC_014034/1446427‑1446627
                                                                                                    |                                                                                                    
tctcGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgcc                                                                                                      >  2:1119932/1‑101 (MQ=255)
tctcGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgcc                                                                                                      >  1:3613710/1‑101 (MQ=255)
tctcGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgcc                                                                                                      <  1:3413104/101‑1 (MQ=255)
  tcGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgc                                                                                                    <  2:2898910/101‑1 (MQ=255)
   cggcggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCNCGGGcgccttcgccgcc                                                                                                   >  2:483694/1‑101 (MQ=255)
   cggcggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgcc                                                                                                   <  2:2463391/101‑1 (MQ=255)
     gcggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgcctt                                                                                                 <  2:1349159/101‑1 (MQ=255)
      cggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccgccttc                                                                                                >  2:3559220/1‑101 (MQ=255)
      cggGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGcgccttcgccaccttc                                                                                                <  1:289810/101‑1 (MQ=255)
         gggCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTcgcg                                                                                             >  2:1275886/1‑101 (MQ=255)
             cTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtgg                                                                                         >  2:1193378/1‑101 (MQ=255)
             cTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtgg                                                                                         >  2:4025526/1‑101 (MQ=255)
              tCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtggt                                                                                        <  1:3285960/101‑1 (MQ=255)
              tCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtggt                                                                                        <  2:3047779/101‑1 (MQ=255)
                gACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCtggtgg                                                                                      <  2:2783859/101‑1 (MQ=255)
                  ccGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGgcg                                                                                    >  1:481847/1‑101 (MQ=255)
                  ccGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGgcg                                                                                    <  2:1851851/101‑1 (MQ=255)
                   cGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGgcgc                                                                                   <  1:3320408/101‑1 (MQ=255)
                     gcgcGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCtt                                                                                 <  1:1813274/101‑1 (MQ=255)
                     gcgcGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCtt                                                                                 <  2:3642707/101‑1 (MQ=255)
                      cgcgTTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCttt                                                                                >  1:3291161/1‑101 (MQ=255)
                       gcgGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCtttt                                                                               <  1:3170588/101‑1 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTGGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGt                                                                             >  1:2292459/1‑101 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGt                                                                             >  2:2376428/1‑101 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGt                                                                             >  1:4434784/1‑101 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGt                                                                             >  2:2846038/1‑101 (MQ=255)
                         ggTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGt                                                                             <  1:1275979/101‑1 (MQ=255)
                          gTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTc                                                                            <  2:4251444/101‑1 (MQ=255)
                            ggCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCGTGGGGGCTTTGGCGGTGGCCTTCGCCCCCTTCGCGCTGGTGGCGGTTATGCCGa                                                                          >  2:2303703/1‑101 (MQ=14)
                            ggCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGa                                                                          >  1:2085478/1‑101 (MQ=255)
                             gCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGaa                                                                         >  2:2021977/1‑101 (MQ=255)
                               ggTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACg                                                                       <  2:2256020/101‑1 (MQ=255)
                                gTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACgg                                                                      <  1:1300229/101‑1 (MQ=255)
                                 tCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgc                                                                     >  1:293082/1‑101 (MQ=255)
                                 tCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgc                                                                     <  1:2794014/101‑1 (MQ=255)
                                  cATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGgcg                                                                    >  2:1607308/1‑101 (MQ=255)
                                    tCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCTATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGcgcg                                                                  <  1:3244732/101‑1 (MQ=255)
                                     cGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGa                                                                 >  2:704853/1‑101 (MQ=255)
                                     cGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGGGGCGCTTTTGTCGAACGGCGCGa                                                                 >  2:1537330/1‑101 (MQ=255)
                                       tGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGAcc                                                               <  2:2410116/101‑1 (MQ=255)
                                         cTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAg                                                             >  1:92338/1‑101 (MQ=255)
                                         cTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAg                                                             >  1:1008854/1‑101 (MQ=255)
                                           gggCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCg                                                           <  1:2545141/101‑1 (MQ=255)
                                             gCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCggg                                                         >  1:3422646/1‑101 (MQ=255)
                                              cATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGc                                                        <  2:2096449/101‑1 (MQ=255)
                                              cATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGc                                                        >  2:4523589/1‑101 (MQ=255)
                                               aTCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGcc                                                       <  2:3290916/101‑1 (MQ=255)
                                               aTCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGcc                                                       <  2:4401955/101‑1 (MQ=255)
                                                 tcggccgggcgggCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGa                                                     >  1:1923437/6‑101 (MQ=17)
                                                     ccgggcgggCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACca                                                 <  1:3705220/100‑1 (MQ=11)
                                                       gggcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACcaca                                               <  2:3754061/101‑1 (MQ=14)
                                                       gggcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACcaca                                               <  2:566861/101‑1 (MQ=38)
                                                         gcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACg                                             <  1:3644261/101‑1 (MQ=14)
                                                         gcgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACg                                             <  1:973091/101‑1 (MQ=38)
                                                          ggggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGa                                            <  1:1367567/100‑1 (MQ=38)
                                                          cgggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGa                                            <  2:4464663/101‑1 (MQ=14)
                                                           gggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGAt                                           >  2:2666565/1‑101 (MQ=14)
                                                            ggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATc                                          >  1:2208649/1‑101 (MQ=38)
                                                            ggcTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGGGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATc                                          >  2:2319370/1‑101 (MQ=38)
                                                             gctgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATcc                                         <  1:1617890/101‑1 (MQ=38)
                                                             gctgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATcc                                         >  1:4275044/1‑101 (MQ=38)
                                                              ctgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCt                                        <  1:2907975/101‑1 (MQ=14)
                                                              ctgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCt                                        >  1:2688668/1‑101 (MQ=14)
                                                              ctgtcgctgtcCATGGGGGCTTGCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCt                                        <  1:3923382/101‑1 (MQ=38)
                                                               tgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTc                                       <  2:3532754/101‑1 (MQ=14)
                                                               tgtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTc                                       <  2:3669558/101‑1 (MQ=14)
                                                                gtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcg                                      <  1:2349508/101‑1 (MQ=38)
                                                                gtcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcg                                      <  2:1768619/101‑1 (MQ=38)
                                                                 tcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcgc                                     >  1:1639016/1‑101 (MQ=14)
                                                                 tcgctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTcgc                                     >  2:761664/1‑101 (MQ=38)
                                                                   gctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCgg                                   <  1:1553425/101‑1 (MQ=14)
                                                                   gctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCTGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCgg                                   <  2:108538/101‑1 (MQ=14)
                                                                    ctgtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCggg                                  >  1:3441972/1‑101 (MQ=37)
                                                                      gtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCggggg                                >  2:884043/1‑101 (MQ=34)
                                                                      gtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCggggg                                >  1:3662167/1‑101 (MQ=34)
                                                                      gtcCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCggggg                                >  2:2725656/1‑101 (MQ=34)
                                                                        ccATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTc                              <  1:742626/101‑1 (MQ=32)
                                                                           tGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACGCGATCCTCGCGGGGGTcgcc                           >  2:3926589/1‑101 (MQ=17)
                                                                             ggggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGGcgccgc                         >  1:718809/1‑101 (MQ=17)
                                                                               ggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCt                       <  1:3085361/101‑1 (MQ=32)
                                                                               ggCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCt                       <  1:3905821/101‑1 (MQ=32)
                                                                                gCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTc                      <  1:1716265/101‑1 (MQ=32)
                                                                                gCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTc                      <  2:4173603/101‑1 (MQ=32)
                                                                                   ttCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCa                   <  1:1120021/101‑1 (MQ=32)
                                                                                   ttCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCa                   >  1:3110822/1‑101 (MQ=33)
                                                                                   ttCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCa                   <  1:1512019/101‑1 (MQ=32)
                                                                                     cgcgGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGc                 >  2:3044064/1‑101 (MQ=32)
                                                                                        gggCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCttt              >  2:144008/1‑101 (MQ=32)
                                                                                        gggCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCttt              >  2:38713/1‑101 (MQ=32)
                                                                                        gggCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCttt              >  2:3097443/1‑101 (MQ=32)
                                                                                        gggCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCttt              >  2:2576719/1‑101 (MQ=32)
                                                                                          gcgcCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTc            <  1:2980564/101‑1 (MQ=32)
                                                                                            gccttcgccgccttcgcTCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAgtcttccaa          >  1:4095068/1‑93 (MQ=11)
                                                                                             ccttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAAc         <  1:4204911/101‑1 (MQ=32)
                                                                                             ccttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAAc         <  2:918464/101‑1 (MQ=32)
                                                                                               ttcgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGGCGGGGTCGCCGCCTCCCAGCTTTTCAAcgc       >  1:2839225/1‑101 (MQ=255)
                                                                                                 cgccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACgcgc     >  2:1907742/1‑101 (MQ=32)
                                                                                                  gccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCACAGCTTTTCAACGCGCt    >  2:1651191/1‑101 (MQ=17)
                                                                                                   ccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTg   <  1:621018/101‑1 (MQ=31)
                                                                                                   ccgccttcgcGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTg   <  1:1243292/101‑1 (MQ=31)
                                                                                                    |                                                                                                    
TCTCGGCGGGGGCCTCGACCGGCGCGGTGGCGGTCATCGTGCTGGGCATCGGCGCGGGCGGGCTGTCGCTGTCCATGGGGGCTTTCGCGGGCGCCTTCGCCGCCTTCGCGCTGGTGGCGCTTTTGTCGAACGGCGCGACCAGCGGGCCGAACCACACGATCCTCGCGGGGGTCGCCGCCTCGCAGCTTTTCAACGCGCTGA  >  NC_014034/1446427‑1446627

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG