Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,507,982 G→C 100% R80R (CGG→CGC RCAP_rcc01400 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,507,9820G→C100.0% 810.8 217R80R (CGG→CGCRCAP_rcc01400signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (105/112):  ref base (0/0):  total (105/112)

Only 100 of 230 total aligned reads displayed.

CTGATCCGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGGTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGCCCGG  >  NC_014034/1507884‑1508079
                                                                                                  |                                                                                                 
cTGATCCGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCtt                                                                                                 >  2:1145106/1‑101 (MQ=255)
 ggATCCGCGCCATGGGCAGCCGCCCGGCCGCCGATCCCGCGCGGCTGATCACCGACCTGCGCGGCGGCATCGTGCGGCGGCGCCATGTCCGCATCCGCTTc                                                                                                <  2:1722122/100‑1 (MQ=255)
   aTCCGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGt                                                                                              <  1:294782/101‑1 (MQ=255)
   aTCCGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGt                                                                                              <  1:3388022/101‑1 (MQ=255)
   aTCCGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGt                                                                                              <  1:1180995/101‑1 (MQ=255)
    tCCGCGACCGGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTc                                                                                             <  2:3452659/101‑1 (MQ=255)
     ccGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTcc                                                                                            <  1:61107/101‑1 (MQ=255)
      cgcgACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTccc                                                                                           <  2:2044883/101‑1 (MQ=255)
       gcgACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTcccc                                                                                          <  1:970438/101‑1 (MQ=255)
       gcgACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTcccc                                                                                          <  1:2164392/101‑1 (MQ=255)
        cgACCTGGTCATACGCCCGGCCGGTGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCccg                                                                                         <  2:2737238/101‑1 (MQ=255)
        cgACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCccg                                                                                         <  1:2438259/101‑1 (MQ=255)
          aCCGGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCccgcc                                                                                       <  1:4103918/101‑1 (MQ=255)
           ccgggTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCccgcca                                                                                      <  2:559386/98‑2 (MQ=255)
              ggTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACg                                                                                   >  2:3304469/1‑101 (MQ=255)
              ggTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACg                                                                                   >  1:1658376/1‑101 (MQ=255)
              ggTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACg                                                                                   >  1:1439200/1‑101 (MQ=255)
                tCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGc                                                                                 <  1:2333031/101‑1 (MQ=255)
                 cAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGcc                                                                                <  2:401117/101‑1 (MQ=255)
                  aGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCt                                                                               >  2:3711561/1‑101 (MQ=255)
                      gCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTcc                                                                           >  1:860773/1‑101 (MQ=255)
                       ccGGGCCGACGACCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTCTccc                                                                          <  2:948654/101‑1 (MQ=255)
                        cGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTcccc                                                                         <  2:1236688/101‑1 (MQ=255)
                        cGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTcccc                                                                         >  2:792835/1‑101 (MQ=255)
                        cGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTcccc                                                                         >  1:4070284/1‑101 (MQ=255)
                        cGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTcccc                                                                         >  1:2836191/1‑101 (MQ=255)
                         gggCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCg                                                                        <  1:3336051/101‑1 (MQ=255)
                           gCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGt                                                                      >  2:4528130/1‑101 (MQ=255)
                           gCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGt                                                                      <  2:3489592/101‑1 (MQ=255)
                            ccGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGtt                                                                     >  2:1893262/1‑101 (MQ=255)
                            ccGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGtt                                                                     <  2:3137859/101‑1 (MQ=255)
                            ccGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGtt                                                                     <  1:2285314/101‑1 (MQ=255)
                             cgacgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTg                                                                    <  1:3006177/101‑1 (MQ=255)
                              gacgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGa                                                                   >  1:2705233/1‑101 (MQ=255)
                              gacgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGa                                                                   >  1:3818515/1‑101 (MQ=255)
                               acgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGAc                                                                  >  1:4616752/1‑101 (MQ=255)
                                cgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGAcc                                                                 >  1:1831720/1‑101 (MQ=255)
                                cgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGAcc                                                                 >  1:2025696/1‑101 (MQ=255)
                                cgaTCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGAcc                                                                 <  2:3614969/101‑1 (MQ=255)
                                 gaTCCCGCGCGGCTGATCATCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCt                                                                <  1:3186082/101‑1 (MQ=255)
                                  tccccTCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTc                                                               <  1:377976/99‑1 (MQ=255)
                                  aTCCCGCGCGGCTGATCACCGATCTGCGCGACGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTc                                                               >  1:4152305/1‑101 (MQ=255)
                                     ccGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCACCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTcccc                                                            <  2:1513068/101‑1 (MQ=255)
                                           ggCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCg                                                      <  1:2609066/101‑1 (MQ=255)
                                             cTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCa                                                    >  2:1801522/1‑101 (MQ=255)
                                             cTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCa                                                    >  2:4119243/1‑101 (MQ=255)
                                              tGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCAt                                                   >  2:1039486/1‑101 (MQ=255)
                                              tGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCAt                                                   >  2:664723/1‑101 (MQ=255)
                                              tGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCAt                                                   <  2:602407/101‑1 (MQ=255)
                                              tGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCAt                                                   <  1:342392/101‑1 (MQ=255)
                                               gATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATg                                                  >  2:4294468/1‑101 (MQ=255)
                                               gATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATg                                                  <  1:397642/101‑1 (MQ=255)
                                                aTCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATgg                                                 <  1:1401348/101‑1 (MQ=255)
                                                aTCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATgg                                                 >  1:184584/1‑101 (MQ=255)
                                                aTCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATgg                                                 >  1:3650485/1‑101 (MQ=255)
                                                  cACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCg                                               >  1:3377349/1‑101 (MQ=255)
                                                  cACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCg                                               <  2:2854815/101‑1 (MQ=255)
                                                  cACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGGCCCCGGTTAACCTCCCCACACCGCATGGCg                                               >  2:4500506/1‑101 (MQ=255)
                                                      gATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGc                                           >  1:917041/1‑101 (MQ=255)
                                                         cTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTAGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGa                                        <  1:1145198/101‑1 (MQ=255)
                                                          tGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGaa                                       >  2:2630610/1‑101 (MQ=255)
                                                             gcggcgGCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCg                                    <  1:1019445/101‑1 (MQ=255)
                                                              cggcggCATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCgg                                   >  1:3242782/1‑101 (MQ=255)
                                                                 cggcATCGTGCGGCCGCCCCATGCCCGCACCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTcc                                <  2:2092371/101‑1 (MQ=255)
                                                                 cggcATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTcc                                >  2:1535782/1‑101 (MQ=255)
                                                                  ggcATCGTGGAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTccc                               <  1:691737/101‑1 (MQ=255)
                                                                  ggcATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTccc                               >  1:108829/1‑101 (MQ=255)
                                                                  ggcATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTccc                               >  2:688468/1‑101 (MQ=255)
                                                                  ggcATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTccc                               <  2:3745101/101‑1 (MQ=255)
                                                                  ggcATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTccc                               <  2:2666777/101‑1 (MQ=255)
                                                                   gcATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCg                              >  1:4228756/1‑101 (MQ=255)
                                                                    cATCGTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCgg                             >  1:3584717/1‑101 (MQ=255)
                                                                        gTGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTg                         <  1:3888337/101‑1 (MQ=255)
                                                                         tGCAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTgg                        >  1:2854583/1‑101 (MQ=255)
                                                                           cAGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGtt                      >  2:4168253/1‑101 (MQ=255)
                                                                            aGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGttt                     >  1:354340/1‑101 (MQ=255)
                                                                            aGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGttt                     <  1:4177101/101‑1 (MQ=255)
                                                                            aGCCGCGCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCACGGCGACGCCGAACCGGTCCCGGGCTGGttt                     >  1:2718806/1‑101 (MQ=255)
                                                                              gcgcgCCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGGTGGTTtct                   <  2:1749970/100‑1 (MQ=255)
                                                                                 cgcCATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACCCCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGa                <  1:714714/101‑1 (MQ=255)
                                                                                    cATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTg             <  2:2937387/101‑1 (MQ=255)
                                                                                    cATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTg             <  2:4479464/101‑1 (MQ=255)
                                                                                    cATGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTg             >  2:3358119/1‑101 (MQ=255)
                                                                                     aTGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTgg            >  1:3962041/1‑101 (MQ=255)
                                                                                     aTGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTgg            >  1:4587488/1‑101 (MQ=255)
                                                                                      tGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTggg           >  1:1792958/1‑101 (MQ=255)
                                                                                      tGTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTggg           >  2:2882881/1‑101 (MQ=255)
                                                                                      tGTCCGCATCCGCTTCGCCCCCGCCGACGGCCGGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGGTTCTCGATTggg           >  2:1432325/1‑101 (MQ=255)
                                                                                       gTCCGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGt          <  2:4261443/101‑1 (MQ=255)
                                                                                         ccGCATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCa        >  2:2466324/1‑101 (MQ=255)
                                                                                          cGCATCCGCTTCGTCCCCGCCGGCGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAg       <  1:1535923/101‑1 (MQ=255)
                                                                                            cttCCGCTTCCCCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGcc     <  2:916961/99‑1 (MQ=255)
                                                                                            cATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGcc     <  1:805091/101‑1 (MQ=255)
                                                                                            cATCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACACCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGcc     >  2:2057399/1‑101 (MQ=255)
                                                                                             ctCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGccc    <  2:4328913/100‑1 (MQ=255)
                                                                                             aTCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGccc    >  2:3240501/1‑101 (MQ=255)
                                                                                             aTCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGccc    <  2:3683607/101‑1 (MQ=255)
                                                                                              tCCGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGCCCg   <  1:1756787/101‑1 (MQ=255)
                                                                                               ccGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGCCCgg  <  1:4376540/101‑1 (MQ=255)
                                                                                               ccGCTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGCCCgg  >  1:264739/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTGATCCGCGACCTGGTCAGACGCCGGGCCGACGATCCCGCGCGGCTGATCACCGATCTGCGCGGCGGCATCGTGCAGCCGCGCCATGTCCGCATCCGGTTCGTCCCCGCCGACGGCCTGTCCCCGGTTGACCTCCCCACACCGCATGGCGACGCCGAACCGGTCCCGGGCTGGTTTCTCGATTGGGTCAGCCCGG  >  NC_014034/1507884‑1508079

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG