Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,933 G→C 100% intergenic (+203/‑144) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9330G→C100.0% 429.9 118intergenic (+203/‑144)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (43/75):  ref base (0/0):  total (43/75)

Only 100 of 135 total aligned reads displayed.

CCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCG  >  NC_014034/1607835‑1608031
                                                                                                  |                                                                                                  
ccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGGTGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGccc                                                                                               >  1:3921516/1‑100 (MQ=255)
ccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCt                                                                                               <  2:828681/101‑1 (MQ=255)
 cACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTg                                                                                              <  1:346658/101‑1 (MQ=255)
  aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGGGGGC.AGACCGGGCCCG.CCGCCGCCTGt                                                                                             >  1:962477/1‑101 (MQ=255)
   cGCCCGCATTGTGGCCGCCGCGCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTc                                                                                            <  2:3959872/101‑1 (MQ=255)
    gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                           <  2:1523568/101‑1 (MQ=255)
    gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                           <  2:3694630/101‑1 (MQ=255)
    ccccGAATTGTGGCCCCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGCCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                           <  2:3892723/100‑1 (MQ=255)
     cccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                                                          >  1:3550410/1‑100 (MQ=255)
      ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTCtcc                                                                                         >  1:1712488/1‑99 (MQ=255)
      ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                                         <  2:1786624/101‑3 (MQ=255)
        gCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccga                                                                                       <  2:241938/101‑5 (MQ=255)
         cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                                                     >  2:548394/1‑101 (MQ=255)
         cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGAc                                                                                     >  1:4336885/1‑101 (MQ=255)
         cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.CGGAATGCGGGC.AGACCGGGCC.GACCGCCCCCTGTCTCccac                                                                                      >  1:2353902/1‑99 (MQ=255)
          aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                                                    <  1:2044984/101‑1 (MQ=255)
           ttGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                                                   >  1:219991/1‑101 (MQ=255)
           ttGGGGCCGCCGCCCCCCAGAACGCGCCCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCt                                                                                   <  1:3230983/101‑1 (MQ=255)
             gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCctt                                                                                 >  1:2905672/1‑101 (MQ=255)
              tgGCCGCCGCGCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                                                <  1:4652107/101‑1 (MQ=255)
              tgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCcttt                                                                                >  2:4452667/1‑101 (MQ=255)
              tgGCCGCCGCCCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                                                <  1:4372711/101‑1 (MQ=255)
               ggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTc                                                                               >  1:3448588/1‑101 (MQ=255)
                  cgccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGG.GAGGCCGGGCC.GACCGCGGCCTGTC.TCCGACCTTTTcccc                                                                            >  1:1192652/1‑100 (MQ=255)
                   gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGCCCTTTTCCCac                                                                           >  2:643176/1‑100 (MQ=255)
                   gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                           <  2:352729/101‑1 (MQ=255)
                   gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                           >  1:3610407/1‑101 (MQ=255)
                     cgcgCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCg                                                                         <  2:1542498/101‑1 (MQ=255)
                     cgcgCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCg                                                                         >  2:3353912/1‑101 (MQ=255)
                        gcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                                                      <  1:1701727/101‑1 (MQ=255)
                         ccccAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATc                                                                     <  1:147809/101‑1 (MQ=255)
                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  1:3029087/101‑1 (MQ=255)
                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  1:3340655/101‑1 (MQ=255)
                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  1:231043/101‑1 (MQ=255)
                            cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  1:485857/101‑1 (MQ=255)
                            cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  2:1666901/101‑1 (MQ=255)
                            cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  2:4247842/101‑1 (MQ=255)
                            cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  1:3005649/101‑1 (MQ=255)
                             aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGAACGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                                 >  2:2903884/1‑101 (MQ=255)
                              gAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                <  1:3063061/101‑1 (MQ=255)
                              gAACGCCCGCCCCGCCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                                                <  2:4489537/101‑1 (MQ=255)
                               aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGGC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgccc                                                               >  2:2128413/1‑99 (MQ=255)
                                ccgcgccCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGAc                                                              <  1:940401/100‑1 (MQ=255)
                                     cgcCCCGGCCCCTCAGGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACGGGGCCGG.CCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGt                                                         <  2:1730577/101‑1 (MQ=255)
                                      gcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                                        >  2:2728499/1‑101 (MQ=255)
                                      gcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCCTTTCCCAGCGGATCCCGCGACgggggg                                                        >  1:2487382/1‑99 (MQ=255)
                                      gcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGGC.TCCGACCTTTTCCCAGCGGATCCCGCGACgggggg                                                        >  1:1046960/1‑99 (MQ=255)
                                      cccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                                        <  1:2013797/100‑1 (MQ=255)
                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                      <  2:608189/101‑1 (MQ=255)
                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCCGCGGATCCCGCGACGGGGTGCgg                                                     >  2:605665/1‑100 (MQ=255)
                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGG.GAGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGa                                                     >  1:1829644/1‑101 (MQ=255)
                                          cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCgcc                                                    >  1:4172411/1‑99 (MQ=255)
                                           ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACg                                                   >  1:521924/1‑101 (MQ=255)
                                            gCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGc                                                  <  2:4424900/101‑1 (MQ=255)
                                             ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcc                                                 <  2:2989015/101‑1 (MQ=255)
                                             ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcc                                                 >  2:2203950/1‑101 (MQ=255)
                                             ccccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcc                                                 <  2:800672/101‑1 (MQ=255)
                                              cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                                                >  1:1798765/1‑101 (MQ=255)
                                              cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCCt                                                >  2:4131705/1‑101 (MQ=255)
                                              cccTCAAGGACGGCGGCGG.CCGGGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                                                <  2:4522435/101‑1 (MQ=255)
                                               ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                               <  2:3242957/101‑2 (MQ=255)
                                                cTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                              <  1:138318/101‑3 (MQ=255)
                                                  cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGGC.GCCCCCCGGCTGTC.TCCGACCTTTTCCCAGCGGAGCCCGCGGCGGGGCGCGACCCCtgcgg                                            >  1:353538/1‑97 (MQ=255)
                                                  cAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcgg                                            >  1:302080/1‑97 (MQ=255)
                                                    aGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCCCGACGGGGCTCGACGCC.Tgcggcg                                         >  2:3017872/1‑101 (MQ=255)
                                                     ggATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGgcgc                                        >  1:866876/1‑101 (MQ=255)
                                                      gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                       >  1:2808120/1‑101 (MQ=255)
                                                       aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccg                                      >  1:4308185/1‑101 (MQ=255)
                                                       aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgccg                                      <  2:219967/101‑1 (MQ=255)
                                                         ggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCgccgcc                                    >  1:1376706/1‑101 (MQ=255)
                                                         ggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCC.TGCGGCgccgcc                                    >  1:3205980/1‑101 (MQ=255)
                                                          gcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc                                   <  1:3533815/101‑1 (MQ=255)
                                                           cgggggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  2:176986/101‑1 (MQ=255)
                                                           cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  >  1:1578120/1‑101 (MQ=255)
                                                           cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  1:3417800/101‑1 (MQ=255)
                                                            ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa                                 >  1:4288474/1‑101 (MQ=255)
                                                            ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa                                 >  2:1999837/1‑101 (MQ=255)
                                                            cggcggcaggggAATGGGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  1:4382580/93‑1 (MQ=255)
                                                             gcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaa                                <  2:2694659/101‑1 (MQ=255)
                                                             gcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaa                                <  1:1970222/101‑1 (MQ=255)
                                                               gggcacgggAATGCGGGCG.GA.CGGGCCCGACCGCCCCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaaa                               <  1:2672938/95‑1 (MQ=255)
                                                               ggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg                              >  1:4404887/1‑101 (MQ=255)
                                                                    cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGACAagggg                          >  2:4260919/2‑101 (MQ=255)
                                                                    cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggggg                          >  2:1274486/2‑101 (MQ=255)
                                                                     gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaaaaagcgg                         >  1:4017187/1‑95 (MQ=255)
                                                                      ggAATGCGGGC.AGCCCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc                        <  2:1712377/101‑1 (MQ=255)
                                                                      ggAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc                        <  2:110690/101‑1 (MQ=255)
                                                                      ggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGc                        <  1:433475/101‑1 (MQ=255)
                                                                       gAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGacaaacgcgcg                       >  2:3677428/1‑91 (MQ=255)
                                                                        aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGGTg                      >  1:3185370/1‑101 (MQ=255)
                                                                        aaTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTg                      >  2:1155749/1‑101 (MQ=255)
                                                                                 cagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcccc              >  1:3357160/2‑101 (MQ=255)
                                                                                  agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgggcggcgcc             >  2:2238130/1‑96 (MQ=255)
                                                                                   gaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcccccc            <  2:4346130/101‑1 (MQ=255)
                                                                                     ccGGGCC.GTCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctc          <  2:2353740/101‑1 (MQ=255)
                                                                                      cGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctct         >  1:2948007/1‑101 (MQ=255)
                                                                                       gggCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCtt        <  1:3327976/101‑1 (MQ=255)
                                                                                         ggccgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTg       <  2:3610139/97‑1 (MQ=255)
                                                                                         ggcGGCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTg       <  2:4404595/99‑1 (MQ=255)
                                                                                            cgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTggtc    >  1:1156254/2‑99 (MQ=38)
                                                                                                  |                                                                                                  
CCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCG  >  NC_014034/1607835‑1608031

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG