Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,669,016 T→C 100% K383E (AAG→GAG)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,669,0160T→C100.0% 833.7 218K383E (AAG→GAG) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (121/96):  ref base (0/0):  total (122/96)

Only 100 of 242 total aligned reads displayed.

GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668918‑1669113
                                                                                                  |                                                                                                 
gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                                                 <  2:1080803/101‑1 (MQ=255)
gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCACCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                                                 <  1:4004439/101‑1 (MQ=255)
gCGGCTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                                                 <  1:4196987/101‑1 (MQ=255)
 cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                                                <  2:2052390/101‑1 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                              <  2:4201539/101‑1 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                              >  2:3607379/1‑101 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                              <  1:4330406/101‑1 (MQ=255)
   gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                              >  1:2565904/1‑101 (MQ=255)
    ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                                             >  1:860862/1‑101 (MQ=255)
    ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                                             >  1:2746540/1‑101 (MQ=255)
       ggCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTc                                                                                          >  1:530039/1‑101 (MQ=255)
        gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                                         <  2:2842089/101‑1 (MQ=255)
        gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                                         <  1:3849720/101‑1 (MQ=255)
         cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                                        >  2:865822/1‑101 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCAAATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAg                                                                                       <  2:3229417/101‑1 (MQ=255)
           ccTCGAAGCCCAGATATTGCGCCGCACACATCTGATGATGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCGTCCAGCCGCTCCAgc                                                                                      >  2:416605/1‑101 (MQ=255)
            cTCGAAGCCCAGATATTGCGCCGCCCACATATGATGCTGGCCGACTTCGGTGGTGCTGTGGTGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcg                                                                                     <  2:3837353/101‑1 (MQ=255)
             tCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcgc                                                                                    >  1:120184/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  <  1:3095884/101‑1 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  <  2:2290059/101‑1 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  >  2:685685/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTAAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  >  2:4031275/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 <  1:4371057/101‑1 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:463963/1‑101 (MQ=255)
                 aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                <  1:1480156/101‑1 (MQ=255)
                  gCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                                               <  2:2189087/101‑1 (MQ=255)
                  gCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                                               >  1:354845/1‑101 (MQ=255)
                    ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                             >  2:1762305/1‑101 (MQ=255)
                     cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                                            >  1:2067120/1‑101 (MQ=255)
                     cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                                            <  2:100214/101‑1 (MQ=255)
                     cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                                            >  2:3404082/1‑101 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  2:1447642/101‑1 (MQ=255)
                          atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       >  1:1007993/1‑101 (MQ=255)
                           ttGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                                                      <  1:3620360/101‑2 (MQ=255)
                            tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                     >  1:2609391/1‑99 (MQ=255)
                             gcgcCGCCCACATCTGTTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGTCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                                                    <  2:2789289/101‑1 (MQ=255)
                                ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                                                 <  2:1174870/101‑1 (MQ=255)
                                ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                                                 <  1:177807/101‑1 (MQ=255)
                                ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                                                 <  2:3276167/101‑1 (MQ=255)
                                 cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                                                <  2:2314904/101‑1 (MQ=255)
                                 cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                                                <  2:798752/101‑1 (MQ=255)
                                  gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                                               <  2:1054726/101‑1 (MQ=255)
                                      ccaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                           <  2:1649831/100‑1 (MQ=255)
                                      acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                           >  1:156641/1‑101 (MQ=255)
                                        aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         <  2:2160489/101‑1 (MQ=255)
                                          cTGATGGTGGCCGACTTGGGTGGTGATGTAGCTGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                                                       <  2:442628/101‑1 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGGGATGTGCGGGTTGCCCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      <  2:4637225/101‑1 (MQ=255)
                                            ggggCTGGCCGACTTCGGTGTTGATGCCGCGTTTCCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                     <  2:721625/98‑1 (MQ=255)
                                            gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                     >  1:4048544/1‑101 (MQ=255)
                                            gATGCGGGCCGGCTTCGGGTGTTATTTAGCGGTTTCCCCCCTTTGTCCGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                     <  1:494794/101‑1 (MQ=255)
                                             aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCgg                                                    <  1:740826/101‑1 (MQ=255)
                                                cTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                                                 >  1:1507283/1‑101 (MQ=255)
                                                 tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                >  2:3496946/1‑101 (MQ=255)
                                                  ggCCGATTTCGGTGGTGATGTAGCGGTTGCGCCCCTTTGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                               <  2:1883131/101‑1 (MQ=255)
                                                   gcggccTTCGGGGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                                              <  2:1328338/96‑1 (MQ=255)
                                                    ccGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                                             <  1:4462119/101‑1 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            >  2:4337544/1‑101 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            >  2:4441584/1‑101 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            >  1:2459804/1‑101 (MQ=255)
                                                      gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                                           >  2:2369785/1‑101 (MQ=255)
                                                       aCTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                          >  1:3157166/1‑101 (MQ=255)
                                                       aCTTCGGGGGTGATGTAGCGGTTGCGCCCTTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                          <  2:4544303/101‑1 (MQ=255)
                                                        cTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                                         <  1:2694863/101‑1 (MQ=255)
                                                         ttCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                                        >  1:681140/1‑101 (MQ=255)
                                                          ttgggtgtgATGTAAGGGTTGGGCCCCTGGGACAGTCCCTCCGGCTGGTCCAGCGCATATTGCGGTTTGATTTTGCTGTCCGAGCCGGTGTAGGCGAGGCa                                       <  2:2705536/95‑1 (MQ=255)
                                                          tCGGTGGTGATGTAGGGGTTGCCCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                                       <  1:4105862/101‑1 (MQ=255)
                                                           gggtggtGATGTAGCGGTTGCCCCCCTTGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                                      <  1:3904077/100‑1 (MQ=255)
                                                           cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                                      <  2:1568059/101‑1 (MQ=255)
                                                            ggtggtGATTTAGCGGTTGCCCACCTTGGACAGGCCATCCAGCCGCTCCAGCGCATATTGGGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     <  1:1962427/101‑1 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     >  1:31864/1‑101 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     >  2:515242/1‑101 (MQ=255)
                                                             gtggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg                                    >  1:4293781/1‑101 (MQ=255)
                                                              tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCATCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg                                   <  1:1424122/101‑1 (MQ=255)
                                                              tggtgATGTACCGGTTGCCCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg                                   <  2:3042727/101‑1 (MQ=255)
                                                                gtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 >  2:3540476/1‑101 (MQ=255)
                                                                gtgATGTAGCGGTTCCTCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 >  2:569596/1‑101 (MQ=255)
                                                                 tgATGTGGCGGTTCCGCCCCTTGGTCCGCCCCCCCAGCCGCCCCCGCCCATATTGCGTTTTGATGATGCTGGCCGAGCCGGTGTAGGCGAGGCAGCGGAtc                                <  1:10711/101‑1 (MQ=255)
                                                                  gATGTAGCGGTGGCGCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               <  1:1910709/101‑1 (MQ=255)
                                                                    tGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCAGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                             >  2:3068745/1‑101 (MQ=255)
                                                                    tGTAGCGGTTGCCCCCCTGGGCCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                             <  1:3600406/101‑1 (MQ=255)
                                                                      tAGCGGTTGCGCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTTGGCGAGGCAGCGGATcttctt                           <  1:2640141/101‑1 (MQ=255)
                                                                        gCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTcc                         >  2:1123501/1‑101 (MQ=255)
                                                                          ggttGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAc                       >  1:1543532/4‑101 (MQ=255)
                                                                            ttGCCCCCCTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                     <  1:302421/100‑1 (MQ=255)
                                                                             tGCGCCCTTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    <  2:3189936/101‑1 (MQ=255)
                                                                             tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  1:3258271/1‑101 (MQ=255)
                                                                             tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  1:2340792/1‑101 (MQ=255)
                                                                               cgcCCCTGGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                  <  2:1592682/101‑1 (MQ=255)
                                                                                cccccTTGGCGAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                 <  2:1224946/100‑1 (MQ=255)
                                                                                  cccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGAt               >  2:255338/1‑101 (MQ=255)
                                                                                  cccTGGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGAt               <  1:1618961/101‑1 (MQ=255)
                                                                                    cTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt             <  1:2507157/101‑1 (MQ=255)
                                                                                    cTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt             >  2:3644077/1‑101 (MQ=255)
                                                                                     ttGGTCGGGGCTCCCAGCCGCTCCGGCGCATATTGGGTTTTGATGATGCTGTCGGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt            <  2:2746342/101‑1 (MQ=255)
                                                                                     ttGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt            <  2:2000377/101‑1 (MQ=255)
                                                                                      tGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           <  2:457048/101‑1 (MQ=255)
                                                                                      tGGTCAGGGCCTGCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           >  1:2529713/1‑101 (MQ=255)
                                                                                         tCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGccc        >  1:4568138/1‑101 (MQ=255)
                                                                                          cAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcccc       >  1:3684431/1‑101 (MQ=255)
                                                                                          cAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcccc       >  2:2443774/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668918‑1669113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG