Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,675,931 A→T 100% Q199L (CAG→CTG)  prfB → peptide chain release factor 2

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,675,9310A→T100.0% 713.0 179Q199L (CAG→CTG) prfBpeptide chain release factor 2
Reads supporting (aligned to +/- strand):  new base (88/91):  ref base (0/0):  total (88/91)

Only 100 of 189 total aligned reads displayed.

ACCGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGACAGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGATC  >  NC_014034/1675834‑1676025
                                                                                                 |                                                                                              
aCCGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGt                                                                                             <  2:1114024/101‑1 (MQ=255)
 ccGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTg                                                                                            <  1:967637/101‑1 (MQ=255)
  cgcgGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGc                                                                                           <  1:1102212/101‑1 (MQ=255)
   gcgGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCg                                                                                          <  1:3174181/101‑1 (MQ=255)
   gcgGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCg                                                                                          <  1:3696135/101‑1 (MQ=255)
   gcgGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCg                                                                                          <  1:3766430/101‑1 (MQ=255)
       gCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGAtt                                                                                      <  2:3728326/101‑1 (MQ=255)
         gATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCggggttc                                                                                    >  1:1745165/1‑96 (MQ=255)
            gAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGcc                                                                                 <  1:1544195/101‑1 (MQ=255)
             aGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGttttggcc                                                                                >  2:3290835/1‑96 (MQ=255)
             aGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTCGccc                                                                                >  2:27223/1‑101 (MQ=255)
              ggCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCt                                                                               >  2:2979218/1‑101 (MQ=255)
              ggCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCt                                                                               <  1:930561/101‑1 (MQ=255)
               gCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTa                                                                              <  1:3145354/101‑1 (MQ=255)
                cGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTTGCCCTAt                                                                             >  2:4236556/1‑101 (MQ=255)
                cGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGGGGGTTTTGCCCtct                                                                             >  1:1904340/1‑99 (MQ=255)
                 ggggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTCGCCCCATg                                                                            >  1:629118/1‑101 (MQ=255)
                  gggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGa                                                                           <  2:2546368/101‑1 (MQ=255)
                  gggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGa                                                                           <  2:1486218/101‑1 (MQ=255)
                  gggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGa                                                                           <  2:1537541/101‑1 (MQ=255)
                  gggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCGCTATGa                                                                           >  1:741529/1‑101 (MQ=255)
                   ggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGAc                                                                          <  2:3978656/101‑1 (MQ=255)
                     aTCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAAACGGAATCGGGCGTGCACCGGCTGGGGCGGCTTTCCCCCTATGACTc                                                                        >  1:3729212/1‑101 (MQ=255)
                          cTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTcggcgg                                                                   >  2:4148245/1‑101 (MQ=255)
                           tCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTTGGCCTATGACTcgggggg                                                                  >  2:716056/1‑100 (MQ=255)
                            cGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGgcggcg                                                                 <  2:3343819/101‑1 (MQ=255)
                              gTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCCATGACTCGgcggggcg                                                               >  2:1894505/1‑101 (MQ=255)
                              gTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCCGAATCGGGCGTGCACCGGCTGGGGGGGATTGTGCCCCATGACTCCgcggggcc                                                               >  2:766178/1‑100 (MQ=255)
                               tCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGgcggcgcgg                                                              <  1:1825426/101‑1 (MQ=255)
                                cGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTCGCCCTATGACTCGggggcgcggg                                                             >  2:2389276/1‑100 (MQ=255)
                                cGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGACGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGgcggcgcggc                                                             <  2:3603546/101‑1 (MQ=255)
                                 gCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGcggcgcggcg                                                            >  2:3379106/1‑101 (MQ=255)
                                  ccTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGcggcgg                                                           >  2:62987/1‑101 (MQ=255)
                                  ccTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCCATGACTCGGCGGGGcggcgg                                                           >  2:4126709/1‑101 (MQ=255)
                                   cTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTATGAATCGGCGGGGCggcggc                                                          >  1:2580582/1‑101 (MQ=255)
                                   cTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGcggcggg                                                          >  1:3242410/1‑100 (MQ=255)
                                    tACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCCGCGGGGcggcggga                                                         >  2:1164251/1‑99 (MQ=255)
                                     aCAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCAt                                                        <  1:3846290/101‑1 (MQ=255)
                                     aCAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCCATGACTCGGCGGCGcggcgggca                                                        >  2:2352233/1‑98 (MQ=255)
                                      cAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGGGGCATa                                                       >  1:2269299/1‑101 (MQ=255)
                                      cAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATa                                                       >  1:3658758/1‑101 (MQ=255)
                                      cAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATa                                                       <  2:4288516/101‑1 (MQ=255)
                                       aaGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTTGCCCTATGACCCGGGGGCGCgggggccaac                                                      >  1:2917763/1‑97 (MQ=255)
                                       aaGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAc                                                      <  1:4316226/101‑1 (MQ=255)
                                          aTTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTATGACTCGGCGGGGCGGCGGCATAcgtc                                                   >  2:2574335/1‑101 (MQ=255)
                                          aTTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcgtc                                                   <  1:2032445/101‑1 (MQ=255)
                                          aTTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcgtc                                                   <  1:3114606/101‑1 (MQ=255)
                                           tttCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcgtcg                                                  <  2:3491177/101‑1 (MQ=255)
                                           tttCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcgtcg                                                  <  2:4213453/101‑1 (MQ=255)
                                            ttCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAccgcct                                                 >  2:1990348/1‑96 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTc                                               <  1:1597299/101‑1 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTc                                               >  2:4424330/1‑101 (MQ=255)
                                               cGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGGATACGTCGTTcc                                              >  2:2155527/1‑100 (MQ=255)
                                               cGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCa                                              <  2:3862348/101‑1 (MQ=255)
                                                    aGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGGGGCATACGGCGTTCAGCTCg                                         >  2:4471025/1‑101 (MQ=255)
                                                     gAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTTGCCCTATGACTCGGCGGCGGGGGGGCATTCGTCGGTCAGCTCgg                                        >  2:4529575/1‑101 (MQ=255)
                                                     gAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCgg                                        <  2:4211458/101‑1 (MQ=255)
                                                     gAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAAGTAGGTCAGCTCgg                                        >  1:58231/1‑101 (MQ=255)
                                                       aTGCCTATGGCTGGCTGAAATCGGAATCGGGCGGGCACCGGCTGGGGCGGATTTCGCCCCAAGACTCGGCGGCGCGGCCGGAAACGTCGTTCACCTCggcc                                      >  1:414765/1‑99 (MQ=255)
                                                       aTGCCTATGGCTGGCTGAAATCAGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTc                                      <  2:3026171/101‑1 (MQ=255)
                                                         gCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTg                                    >  1:1438700/1‑101 (MQ=255)
                                                         gCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTg                                    <  1:716131/101‑1 (MQ=255)
                                                            tATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGt                                 <  1:4352522/101‑1 (MQ=255)
                                                             aTGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTATGACTCGGCGGCGCGGTGGCATACGTCGTTAAGCTCGGGCTggggc                                >  2:1007871/1‑99 (MQ=255)
                                                             aTGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTc                                <  1:2402106/101‑1 (MQ=255)
                                                             aTGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTTAGCTCGGGCTTGGTc                                >  2:882325/1‑101 (MQ=255)
                                                              tggctggcTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCCTACGGCGTTCAGCTCGGGCTGGGGCt                               >  2:3918665/1‑101 (MQ=255)
                                                              tggctggcTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCCGCTCGGGCTGGGGCt                               >  2:3213406/1‑101 (MQ=255)
                                                              tggctgccAGAAATCCGAATCGGGCGTGCACCGGCGGGTGCGGATTTCTCCCTATGCCTCGGCGGCCCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCt                               <  1:3864214/101‑1 (MQ=255)
                                                               ggctggctGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTa                              >  1:1341087/1‑101 (MQ=255)
                                                               ggctggctGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAAGTCGGTCAGCTCGGGCTGGGTCTa                              >  2:4173955/1‑101 (MQ=255)
                                                                gctggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTAt                             <  2:2419962/101‑1 (MQ=255)
                                                                 ctggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGGATACGTCGTTCAGCTCGGTCTGGGTCTATc                            >  1:4588516/1‑101 (MQ=255)
                                                                 ctggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGGCTATc                            >  1:3581742/1‑101 (MQ=255)
                                                                 ctggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGGCTATc                            >  2:1409646/1‑101 (MQ=255)
                                                                  tggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATcc                           <  1:3253070/101‑1 (MQ=255)
                                                                  tggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATcc                           <  1:2164941/101‑1 (MQ=255)
                                                                  tggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATcc                           <  2:1830334/101‑1 (MQ=255)
                                                                  tggctgAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCCTGCGTCGTTCACCTCGGGCTGGGGCTCTcc                           >  1:1036586/1‑101 (MQ=255)
                                                                   ggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCg                          <  2:1627126/101‑1 (MQ=255)
                                                                   ggctgAAATCGGAATCGGGCGTGCACCGGCTGGCGCGGATTTTGCCCTATGACTCGGGGGCGCGGGGGCATACGGCGTTCAGATCGGTCTGGGTCTATCCg                          >  2:2842254/1‑101 (MQ=255)
                                                                      tgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtc                       >  2:9705/1‑101 (MQ=255)
                                                                        aaaTCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtcgt                     <  1:40779/101‑1 (MQ=255)
                                                                        aaaTCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtcgt                     >  2:4071903/1‑101 (MQ=255)
                                                                          atcggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGtcgtcg                   <  2:4615651/101‑1 (MQ=255)
                                                                             ggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGGGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTcgccg                >  2:796482/1‑101 (MQ=255)
                                                                             ggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTcgacg                <  2:2263289/101‑1 (MQ=255)
                                                                              gaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTcgacga               >  1:1730120/1‑101 (MQ=255)
                                                                                atcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGGCGTCCACGACa             >  1:271248/1‑101 (MQ=255)
                                                                                 tcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGGCTATCCGGTCGTCgacgacga            >  2:3437708/1‑99 (MQ=255)
                                                                                   gggCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGGATACGTCGTTCAGCTCGGGCTGGGGCTATCCGGGCGTCGACGACAata          >  2:3086619/1‑101 (MQ=255)
                                                                                      ggTGCACCGGCTGGTGCCGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCg       <  2:1239084/100‑1 (MQ=255)
                                                                                      cGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCg       >  2:3573770/1‑101 (MQ=255)
                                                                                       gTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCga      >  1:1648944/1‑101 (MQ=255)
                                                                                       gTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCga      >  1:4429/1‑101 (MQ=255)
                                                                                        tGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCgag     <  1:2889219/101‑1 (MQ=255)
                                                                                        tGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCgag     <  2:2137921/101‑1 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGAt   <  1:3379356/101‑1 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGAt   <  1:892284/101‑1 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGGCGACGACAATATCGAGAt   >  1:1844968/1‑101 (MQ=255)
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ACCGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGACAGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGATC  >  NC_014034/1675834‑1676025

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG