Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,179 A→C 3.9% intergenic (‑107/+118) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1790A→C 3.9% 12.8 ‑1.2 ‑0.0211intergenic (‑107/+118)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (95/107):  total (101/110)
Fisher's exact test strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.03e-01

Only 100 of 221 total aligned reads displayed.

CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283
                                                                                                  |                                                                                                        
ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTc                                                                                                      <  1:1674959/101‑1 (MQ=255)
   aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCAcc                                                                                                  >  1:1269656/1‑101 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCAccc                                                                                                 >  2:1114933/1‑101 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCAcccc                                                                                                <  1:2800105/101‑2 (MQ=37)
      tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCAccccg                                                                                               >  1:492437/1‑99 (MQ=255)
       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCACCCcgg                                                                                              >  1:591293/1‑101 (MQ=37)
        ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCggg                                                                                              <  1:1048704/101‑1 (MQ=37)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGa                                                                                             <  2:2212519/101‑1 (MQ=255)
           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGata                                                                                           >  2:452068/1‑99 (MQ=255)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGatgtt                                                                                         >  1:2231068/1‑97 (MQ=25)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGatgtt                                                                                         >  1:2166922/1‑97 (MQ=25)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGatgtt                                                                                         <  2:1758377/101‑5 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCTAGGAGGGGAG.AGGGACGC.TGCCCCTCCTGGCCTTTCGG.CCCATTCACCCCGGGAGGtttg                                                                                      >  1:1515959/1‑99 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg                                                                                       >  1:551668/1‑101 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg                                                                                       <  2:1210245/101‑1 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg                                                                                       >  2:4194845/1‑101 (MQ=255)
               tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg                                                                                       >  2:2979233/1‑101 (MQ=255)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATCTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCCCCCCGCCTTTCGGCCCATTCCCCCCCGGGTGGTTGGGc                                                                                    >  1:135955/1‑101 (MQ=255)
                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGcc                                                                                   <  1:715766/101‑1 (MQ=255)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCa                                                                                  <  1:2479879/101‑1 (MQ=25)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCa                                                                                  >  1:2926707/1‑101 (MQ=255)
                     tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGGTGTTTGGGCCaa                                                                                 >  1:673619/1‑101 (MQ=255)
                       tGTTTACTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaagg                                                                               <  1:768081/101‑3 (MQ=16)
                        ttttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCACCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                              <  2:730882/100‑4 (MQ=255)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                              <  1:962852/101‑4 (MQ=255)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                              <  2:3871213/101‑4 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                             <  2:1868248/101‑5 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            >  1:1370152/1‑96 (MQ=255)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                            >  2:2237879/1‑96 (MQ=255)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           >  1:2022913/1‑95 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          <  1:985851/101‑8 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGGTGTTTGGGCCAAGgggaaac                                                                         >  1:3961236/1‑97 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAAGgggaaac                                                                         >  1:336489/1‑97 (MQ=255)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                       <  2:891629/101‑1 (MQ=16)
                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                     >  1:4219327/1‑99 (MQ=12)
                                tCGAATCCTTGCACTAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                    <  2:3180335/101‑4 (MQ=12)
                                tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                    >  1:1724768/1‑98 (MQ=255)
                                tCGAATCCTTGCACGAATTTTACAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                    >  1:3439253/1‑98 (MQ=255)
                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                   >  2:3486355/1‑97 (MQ=255)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                  <  1:1115022/101‑6 (MQ=255)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                 >  1:3626574/1‑95 (MQ=255)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                <  2:3046570/101‑8 (MQ=255)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                >  1:108992/1‑94 (MQ=255)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              <  1:939666/101‑10 (MQ=9)
                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                              <  2:373607/101‑10 (MQ=9)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                             <  1:3125239/101‑11 (MQ=255)
                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                        >  2:3648841/1‑101 (MQ=255)
                                           cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                       <  1:1346254/101‑1 (MQ=255)
                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCCATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.TACCCT.GCTTTTTCtt                                                      >  2:393602/1‑101 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  1:4094066/101‑1 (MQ=255)
                                             cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                     <  1:4065673/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                    <  1:3191089/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                    <  1:2983853/101‑1 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTgg                                                    >  2:3910996/1‑101 (MQ=9)
                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                   >  1:3861251/1‑101 (MQ=255)
                                                aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                  <  2:1678692/101‑1 (MQ=255)
                                                 ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                 >  1:1889287/1‑101 (MQ=9)
                                                  tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA..AACCTTGCTTTTTCTTGGCGCa                                                >  1:3720776/1‑101 (MQ=255)
                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCCATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                               >  1:4403641/1‑101 (MQ=255)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaaa                                             >  2:3339231/1‑100 (MQ=255)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                             <  1:3789785/101‑1 (MQ=9)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                            <  1:179839/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           >  2:1518390/1‑101 (MQ=9)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           <  2:2166772/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           <  2:2614232/101‑1 (MQ=255)
                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                        >  1:4162492/1‑101 (MQ=9)
                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                        <  1:1213397/101‑1 (MQ=255)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                       <  1:4253429/101‑1 (MQ=9)
                                                            ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                      <  1:1548295/101‑1 (MQ=255)
                                                            ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                      >  1:2678334/1‑101 (MQ=12)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                     <  2:4399582/101‑1 (MQ=255)
                                                                   gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 <  2:551620/92‑1 (MQ=9)
                                                                   gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 <  2:3143671/92‑1 (MQ=9)
                                                                   gaggggcgctGCACCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 >  1:2519476/10‑101 (MQ=12)
                                                                     ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..TCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                               <  2:4364600/94‑1 (MQ=255)
                                                                         cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                           <  1:2862021/98‑1 (MQ=16)
                                                                         cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                           <  2:42925/98‑1 (MQ=16)
                                                                          gctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgga                          >  1:1442160/3‑100 (MQ=16)
                                                                            tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                        >  2:4050151/1‑101 (MQ=19)
                                                                            tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                        >  2:920538/1‑101 (MQ=16)
                                                                             gCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                       <  2:2849283/101‑1 (MQ=255)
                                                                              ccccTCCTGGCCTTTCGGCCAATTCTCCCCTGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      <  2:703134/101‑1 (MQ=16)
                                                                              ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      >  2:3703069/1‑101 (MQ=19)
                                                                              ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      >  2:2742293/1‑101 (MQ=16)
                                                                              ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      >  1:2346823/1‑101 (MQ=16)
                                                                               cccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                     <  2:3694153/101‑1 (MQ=16)
                                                                                ccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                    <  2:4433117/101‑1 (MQ=16)
                                                                                     tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc               >  2:383349/1‑101 (MQ=255)
                                                                                     tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc               <  2:2519302/101‑1 (MQ=19)
                                                                                     tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc               >  2:4626795/1‑101 (MQ=255)
                                                                                      ggCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg              <  2:3960951/101‑1 (MQ=19)
                                                                                       gCCTTTCGGCCAATTCCCCCCGTTATGTTTGGGCCAA..GGT.AAAACA.CCTTTTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa             <  1:847025/101‑1 (MQ=255)
                                                                                       gCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa             >  2:2462267/1‑101 (MQ=255)
                                                                                       gCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa             >  1:1646276/1‑101 (MQ=255)
                                                                                        ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa            >  1:4637674/1‑101 (MQ=255)
                                                                                         cTTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           <  1:1087991/101‑1 (MQ=19)
                                                                                         cTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           <  2:1369991/101‑1 (MQ=19)
                                                                                         cTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           >  2:1419420/1‑101 (MQ=255)
                                                                                            tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg        <  2:760006/96‑1 (MQ=19)
                                                                                                 cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc   >  2:1008192/1‑101 (MQ=255)
                                                                                                  |                                                                                                        
CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG