Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,696,184 | C→A | 95.4% | intergenic (‑112/+113) | RCAP_rcc01564 ← / ← mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,696,184 | 0 | C→A | 95.4% | 14.4 0.0 ‑0.3 | 208 | intergenic (‑112/+113) | RCAP_rcc01564/mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Reads supporting (aligned to +/- strand): new base (96/100): ref base (5/6): total (101/107) | ||||||||||
Fisher's exact test strand distribution p-value = 1.00e+00 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.67e-01 |
Only 100 of 218 total aligned reads displayed.
AAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT > NC_014034/1696085‑1696287
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gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCAATTCAcccc < 1:2800105/101‑2 (MQ=37)
ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCggg < 1:1048704/101‑1 (MQ=37)
ccTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCggg > 1:1745125/1‑101 (MQ=255)
cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGa < 2:2212519/101‑1 (MQ=255)
gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGatg < 1:1264316/101‑3 (MQ=25)
aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGatgt > 2:3657744/1‑98 (MQ=255)
aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGatgtt < 2:1758377/101‑5 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg > 1:551668/1‑101 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg > 2:2979233/1‑101 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg < 2:402382/101‑1 (MQ=255)
tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTg > 1:2052590/1‑101 (MQ=255)
aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTggg > 2:451076/1‑101 (MQ=255)
aCGTCTGTTTAATTTCGAATCCTTGCACGAATCTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCCCCCCGCCTTTCGGCCCATTCCCCCCCGGGTGGTTGGGc > 1:135955/1‑101 (MQ=255)
cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGcc < 1:715766/101‑1 (MQ=255)
cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGcc > 2:379954/1‑101 (MQ=25)
gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCa < 1:2479879/101‑1 (MQ=25)
gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCa > 1:2926707/1‑101 (MQ=255)
cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaag > 1:1508755/1‑100 (MQ=21)
tGTTTACTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaagg < 1:768081/101‑3 (MQ=16)
tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaagg > 1:2314313/1‑99 (MQ=255)
ttttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCACCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggt < 2:730882/100‑4 (MQ=255)
gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggt < 2:3871213/101‑4 (MQ=255)
tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtg < 2:1868248/101‑5 (MQ=255)
ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga > 2:2237879/1‑96 (MQ=255)
ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtga > 1:1370152/1‑96 (MQ=255)
tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa > 2:3708085/1‑95 (MQ=255)
aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCCCCCCGGGATGTTTGGGCCaaggtgaaa < 1:4577673/101‑8 (MQ=255)
aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa < 1:985851/101‑8 (MQ=255)
aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGGTGTTTGGGCCAAGgggaaac > 1:3961236/1‑97 (MQ=255)
aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAAGgggaaac > 1:336489/1‑97 (MQ=255)
aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac < 2:891629/101‑1 (MQ=16)
ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac > 1:4219327/1‑99 (MQ=12)
tCGAATCCTTGCACTAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc < 2:3180335/101‑4 (MQ=12)
tCGAATCCTTGCACGAATTTTACAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc > 1:3439253/1‑98 (MQ=255)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt < 1:1115022/101‑6 (MQ=255)
aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg > 1:3626574/1‑95 (MQ=255)
aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg < 1:67973/101‑7 (MQ=12)
aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc > 1:108992/1‑94 (MQ=255)
ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGAGGTTTGGGCCAA..GGTGAAacaccttgctt > 1:2726372/1‑92 (MQ=255)
cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt < 1:1346254/101‑1 (MQ=255)
aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCCATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.TACCCT.GCTTTTTCtt > 2:393602/1‑101 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 1:4094066/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 1:4065673/101‑1 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg < 1:3191089/101‑1 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg < 1:2983853/101‑1 (MQ=255)
aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc > 1:3861251/1‑101 (MQ=255)
aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg < 2:1678692/101‑1 (MQ=255)
aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg < 1:951987/101‑1 (MQ=255)
ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc > 1:1889287/1‑101 (MQ=9)
ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTAGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc > 2:3575452/1‑101 (MQ=9)
tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA..AACCTTGCTTTTTCTTGGCGCa > 1:3720776/1‑101 (MQ=255)
ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCCATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa > 1:4403641/1‑101 (MQ=255)
tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..NGNNNAA.CA.CCTTGNTTTTTCTTGGGGCaaa > 1:4905/1‑101 (MQ=2)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaaa > 2:3339231/1‑100 (MQ=255)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt < 1:3789785/101‑1 (MQ=9)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa > 1:628172/1‑101 (MQ=255)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa < 1:179839/101‑1 (MQ=255)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa < 1:1547415/101‑1 (MQ=255)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc < 2:101686/101‑1 (MQ=255)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc > 2:4253141/1‑101 (MQ=9)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc > 2:1518390/1‑101 (MQ=9)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc < 2:2614232/101‑1 (MQ=255)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc < 2:2166772/101‑1 (MQ=255)
ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc < 2:4429655/101‑1 (MQ=255)
gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc > 1:4162492/1‑101 (MQ=9)
gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc < 1:1213397/101‑1 (MQ=255)
aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct < 1:4253429/101‑1 (MQ=9)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct < 2:4399582/101‑1 (MQ=255)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc < 2:551620/92‑1 (MQ=9)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc > 1:1675858/10‑101 (MQ=16)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc < 1:972764/92‑1 (MQ=12)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc < 2:3143671/92‑1 (MQ=9)
gaggggcgctGCACCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc > 1:2519476/10‑101 (MQ=12)
aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc < 2:1397642/93‑1 (MQ=255)
aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc < 2:3044577/93‑1 (MQ=255)
ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCTCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg < 1:913256/94‑1 (MQ=16)
ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..TCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg < 2:4364600/94‑1 (MQ=255)
tgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg < 2:1442065/98‑1 (MQ=16)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg < 1:2862021/98‑1 (MQ=16)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg < 2:3699720/98‑1 (MQ=16)
gctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgga > 1:1442160/3‑100 (MQ=16)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa > 2:920538/1‑101 (MQ=16)
gCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa > 2:1205929/1‑101 (MQ=19)
gCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa < 2:2849283/101‑1 (MQ=255)
ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg > 1:2346823/1‑101 (MQ=16)
ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg > 2:2742293/1‑101 (MQ=16)
cctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg > 1:2172185/3‑101 (MQ=19)
tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc < 2:2519302/101‑1 (MQ=19)
tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc > 2:383349/1‑101 (MQ=255)
tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc > 2:4626795/1‑101 (MQ=255)
ggCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg < 2:3960951/101‑1 (MQ=19)
gCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa > 2:2462267/1‑101 (MQ=255)
gCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa > 1:1646276/1‑101 (MQ=255)
ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa > 2:711831/1‑101 (MQ=255)
cTTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg < 1:1087991/101‑1 (MQ=19)
cTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg < 2:1369991/101‑1 (MQ=19)
tttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg > 1:3130591/1‑101 (MQ=255)
tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg < 2:760006/96‑1 (MQ=19)
gccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg > 2:4124454/3‑101 (MQ=255)
ttCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc < 2:336457/101‑1 (MQ=25)
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AAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCT > NC_014034/1696085‑1696287
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 19 ≤
ATCG
< 28 ≤
ATCG
< 34 ≤
ATCG
< 41 ≤
ATCG
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