BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,193	A→T	100%	intergenic (‑121/+104)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,193	0	A→T	100.0%	613.8	192	intergenic (‑121/+104)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (89/101): ref base (0/1): total (90/102)

Only 100 of 201 total aligned reads displayed.


TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGT  >  NC_014034/1696097‑1696293


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                                 <  2:402382/101‑1 (MQ=255)


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                                 <  2:1210245/101‑1 (MQ=255)


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                                 >  2:2979233/1‑101 (MQ=255)


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTg                                                                                                 >  1:2052590/1‑101 (MQ=255)


 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                                                >  2:4292770/1‑101 (MQ=25)


  aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTggg                                                                                               <  1:1346861/101‑1 (MQ=25)


   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                              >  1:3143915/1‑101 (MQ=255)


    cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGcc                                                                                             <  1:715766/101‑1 (MQ=255)


     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                                            >  1:978310/1‑101 (MQ=255)


     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                                            >  1:2926707/1‑101 (MQ=255)


     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                                            <  1:2479879/101‑1 (MQ=25)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                          >  1:1508755/1‑100 (MQ=21)


        tGTTTACTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                                                                         <  1:768081/101‑3 (MQ=16)


        tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                                                                         >  1:2314313/1‑99 (MQ=255)


         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                                        <  1:962852/101‑4 (MQ=255)


         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                                        >  1:3644898/1‑98 (MQ=255)


         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                                        <  2:673557/101‑4 (MQ=255)


           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                                      >  1:1370152/1‑96 (MQ=255)


             aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGTCGC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                                    <  2:4384062/101‑8 (MQ=255)


              aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGGTGTTTGGGCCAAGgggaaac                                                                                   >  1:3961236/1‑97 (MQ=255)


              aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAAGgggaaac                                                                                   >  1:336489/1‑97 (MQ=255)


                ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                               >  1:4219327/1‑99 (MQ=12)


                 tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                              >  1:1724768/1‑98 (MQ=255)


                 tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                              >  1:167125/1‑98 (MQ=255)


                 tCGAATCCTTGCACGAATTTTACAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                              >  1:3439253/1‑98 (MQ=255)


                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                             >  2:3486355/1‑97 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                            <  2:1704277/101‑6 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                            <  1:1115022/101‑6 (MQ=255)


                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                           <  1:67973/101‑7 (MQ=12)


                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGGAGTTTGGGCCAAGgggaaacaccttg                                                                             >  1:840174/1‑91 (MQ=255)


                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                          <  2:3046570/101‑8 (MQ=255)


                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                          >  1:108992/1‑94 (MQ=255)


                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                          <  1:4124514/101‑8 (MQ=255)


                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        <  1:939666/101‑10 (MQ=9)


                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        <  2:373607/101‑10 (MQ=9)


                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        <  2:2361167/101‑10 (MQ=9)


                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGAGGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        >  1:2726372/1‑92 (MQ=255)


                        cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                       >  1:3527130/1‑91 (MQ=255)


                        cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                       <  1:3125239/101‑11 (MQ=255)


                            cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                 >  1:2849499/1‑101 (MQ=9)


                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCCATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.TACCCT.GCTTTTTCtt                                                                >  2:393602/1‑101 (MQ=255)


                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                >  1:516253/1‑101 (MQ=255)


                               gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                              <  1:2983853/101‑1 (MQ=255)


                               gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                              <  1:3191089/101‑1 (MQ=255)


                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGAC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                             <  2:1667877/101‑1 (MQ=255)


                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                             >  1:3861251/1‑101 (MQ=255)


                                 aTTTTGCAAGGAGGGGAG.AGGGGAGC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCCA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                            >  2:1797521/1‑101 (MQ=255)


                                  ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                           >  1:1889287/1‑101 (MQ=9)


                                   tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCCTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                          >  2:870626/1‑101 (MQ=255)


                                    ttGCAAGGAGGGGATAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                         <  2:3871389/101‑1 (MQ=255)


                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCCATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                         >  1:4403641/1‑101 (MQ=255)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                       >  2:4629246/1‑101 (MQ=255)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                       <  2:3814493/101‑1 (MQ=9)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                       <  1:3789785/101‑1 (MQ=9)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                      <  1:179839/101‑1 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                      >  1:628172/1‑101 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                      <  1:1547415/101‑1 (MQ=255)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                     >  2:4253141/1‑101 (MQ=9)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                     <  2:2614232/101‑1 (MQ=255)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                     <  2:2166772/101‑1 (MQ=255)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                     <  1:4078852/101‑1 (MQ=255)


                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                   <  1:1076429/101‑1 (MQ=255)


                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                  >  1:4162492/1‑101 (MQ=9)


                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                 <  1:4253429/101‑1 (MQ=9)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                               <  2:4413375/101‑1 (MQ=255)


                                                    gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                           <  2:3143671/92‑1 (MQ=9)


                                                    gaggggcgctGCACCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                           >  1:2519476/10‑101 (MQ=12)


                                                     aggggcgctGCCCCTCCCGACCTTTCG.GCCAATTCACCCCGGGCTGTTTGCGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGTccc                                          >  2:732924/9‑101 (MQ=255)


                                                      ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                         <  1:1620916/94‑1 (MQ=255)


                                                           gctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgga                                    >  1:1442160/3‑100 (MQ=16)


                                                             tGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                  >  2:920538/1‑101 (MQ=16)


                                                              gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGCAGGGCac                                 >  2:3346600/1‑100 (MQ=16)


                                                              gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                 <  2:2849283/101‑1 (MQ=255)


                                                              gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                 <  2:3644630/101‑1 (MQ=255)


                                                               ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                >  1:2346823/1‑101 (MQ=16)


                                                               ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                >  2:3703069/1‑101 (MQ=19)


                                                               ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                >  2:2742293/1‑101 (MQ=16)


                                                               ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                <  1:3760743/101‑1 (MQ=255)


                                                                cccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                               <  2:3694153/101‑1 (MQ=16)


                                                                 ccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                              <  2:4433117/101‑1 (MQ=16)


                                                                      tGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                         <  2:2519302/101‑1 (MQ=19)


                                                                       ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                        <  2:3960951/101‑1 (MQ=19)


                                                                         ccTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                      >  2:711831/1‑101 (MQ=255)


                                                                         ccTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                      >  1:4637674/1‑101 (MQ=255)


                                                                          cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                     <  2:1369991/101‑1 (MQ=19)


                                                                          cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                     >  2:1419420/1‑101 (MQ=255)


                                                                           tttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                    >  2:2228689/1‑101 (MQ=19)


                                                                            ttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                   >  2:3247382/1‑101 (MQ=255)


                                                                              tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg                  <  2:3860966/96‑1 (MQ=19)


                                                                                 gccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg               >  2:4124454/3‑101 (MQ=255)


                                                                                   cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc             >  1:1229759/1‑101 (MQ=255)


                                                                                   cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc             >  2:1008192/1‑101 (MQ=255)


                                                                                    aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc            >  2:2488738/1‑101 (MQ=255)


                                                                                      ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc          >  2:4625903/6‑101 (MQ=255)


                                                                                        caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt        <  1:4050436/98‑1 (MQ=25)


                                                                                         cccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg       <  2:108978/99‑1 (MQ=25)


                                                                                         cACCCCGGGATGTTTGGCCCAA.GGTGAAAC..CCCTTGTTTTTTCTTGGCGAAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt        <  2:1508646/101‑1 (MQ=255)


                                                                                          ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa      <  1:1367221/100‑1 (MQ=37)


                                                                                              gggATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGAGGCCCCCCTGATCGt  >  1:865735/1‑101 (MQ=255)


                                                                                              gggATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGt  >  2:1976331/1‑101 (MQ=255)


TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGT  >  NC_014034/1696097‑1696293

Base quality scores:


ATCG

< 3 ≤


ATCG

< 17 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG