Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,847 G→C 4.0% intergenic (‑100/+93) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8470G→C 4.0% 9.6 ‑0.8 ‑0.0196intergenic (‑100/+93)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (83/105):  total (88/108)
Fisher's exact test strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.24e-01

Only 100 of 199 total aligned reads displayed.

ACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGA  >  NC_014034/1734752‑1734947
                                                                                                  |                                                                                                        
aCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCt                                                                                                      <  1:2977647/101‑1 (MQ=255)
  cTCGGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGc                                                                                                    <  2:260627/101‑1 (MQ=255)
      ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGAc                                                                                                <  2:4610887/101‑1 (MQ=255)
      ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGAc                                                                                                <  2:2230495/101‑1 (MQ=255)
       gggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACt                                                                                               >  1:1201491/1‑101 (MQ=255)
       gggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACt                                                                                               >  1:2346611/1‑101 (MQ=255)
          aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.ccc                                                                                           <  2:4511714/101‑1 (MQ=255)
             ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.cccccc                                                                                        <  2:4404005/101‑1 (MQ=255)
             ggCGCAGCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.cccccc                                                                                        <  1:1754898/101‑1 (MQ=255)
             ggCCCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.cccccc                                                                                        <  2:1436771/101‑1 (MQ=255)
               cgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCgg                                                                                      <  2:946482/101‑1 (MQ=255)
                gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCggg                                                                                     <  2:3905654/101‑1 (MQ=255)
                 cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCCGGa                                                                                    >  2:422033/1‑101 (MQ=255)
                  aTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCCGGat                                                                                   >  2:1787208/1‑101 (MQ=255)
                   tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGata                                                                                  >  1:80430/1‑101 (MQ=255)
                   tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGata                                                                                  <  2:1457478/101‑1 (MQ=255)
                    ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCgggggat                                                                                 >  1:1640963/1‑97 (MQ=255)
                     cTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATAtt                                                                                >  2:1090535/1‑101 (MQ=255)
                      caggcgcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCCTCCGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATAttt                                                                               <  2:616689/96‑1 (MQ=18)
                         cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATg                                                                            <  1:1232154/101‑1 (MQ=255)
                         cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATg                                                                            >  2:3530466/1‑101 (MQ=255)
                          agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATgg                                                                           <  1:117366/101‑1 (MQ=255)
                             cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.aa                                                                       <  2:1629925/101‑1 (MQ=255)
                              ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAg                                                                      >  1:2277184/1‑101 (MQ=255)
                              ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAg                                                                      >  1:4111430/1‑101 (MQ=255)
                               gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGa                                                                     <  1:2761632/101‑1 (MQ=255)
                                ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGG.AAGAt                                                                    >  1:1163249/1‑101 (MQ=255)
                                ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGAt                                                                    <  1:1999012/101‑1 (MQ=255)
                                ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGAt                                                                    >  1:4334206/1‑101 (MQ=255)
                                ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCTCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGAt                                                                    <  2:752858/101‑1 (MQ=255)
                                 gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATg                                                                   <  1:1570152/101‑1 (MQ=255)
                                 gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCCGGCTATTTTTTGG.AAGATg                                                                   >  1:56399/1‑101 (MQ=21)
                                  cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGa                                                                  <  1:1944217/101‑1 (MQ=255)
                                  cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGa                                                                  <  1:2511831/101‑1 (MQ=255)
                                   gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGaa                                                                 <  1:3776779/101‑1 (MQ=255)
                                   gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGaa                                                                 <  1:3516714/101‑1 (MQ=255)
                                    ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGaaa                                                                >  2:681505/1‑101 (MQ=255)
                                    ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGaaa                                                                <  1:3150948/101‑1 (MQ=255)
                                       aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTg                                                             <  2:1980975/101‑1 (MQ=255)
                                       aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTg                                                             >  2:1070973/1‑101 (MQ=255)
                                       aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTg                                                             >  1:1403475/1‑101 (MQ=255)
                                       aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTg                                                             >  2:3435053/1‑101 (MQ=255)
                                       aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGAAATTTATTGG.AAGAAGaaaacg                                                             >  1:1604283/1‑98 (MQ=18)
                                          ccAATGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.gg                                                         <  2:2930853/101‑1 (MQ=255)
                                          ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.gg                                                         >  1:66097/1‑101 (MQ=255)
                                            aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGcc                                                       >  1:4395081/1‑101 (MQ=255)
                                            aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGcc                                                       >  2:197414/1‑101 (MQ=255)
                                            aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGcc                                                       >  2:2059384/1‑101 (MQ=255)
                                                gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCa                                                   <  2:910693/101‑1 (MQ=255)
                                                gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCa                                                   <  1:1068916/101‑1 (MQ=255)
                                                 cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCAt                                                  <  2:2530820/101‑1 (MQ=255)
                                                 cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCAGGATATGTATGGC.AAGATGGAACTGC.GGCCTTCAt                                                  >  2:1880036/1‑101 (MQ=18)
                                                  tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCCGGGAAATTTTTGGC.AAAAAGAAAACGC.GGCCTTCAt                                                  >  1:2399213/1‑101 (MQ=17)
                                                   gccccTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AATATGAAACTGC.GGCCTTCATCt                                                <  2:1116824/97‑1 (MQ=18)
                                                   gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCt                                                <  1:732751/101‑1 (MQ=255)
                                                   gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAAAAGAAACTGC.GGGCTTCATCt                                                >  1:2616302/1‑101 (MQ=18)
                                                    gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCtt                                               <  2:3512624/100‑1 (MQ=255)
                                                    aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCtt                                               <  1:3938117/101‑1 (MQ=255)
                                                    aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGTTGAAACTGC.GGCCTTCATCtt                                               <  2:4459524/101‑1 (MQ=255)
                                                    aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCtt                                               <  2:3722210/101‑1 (MQ=255)
                                                     cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTg                                              <  2:2338294/101‑1 (MQ=255)
                                                     cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTg                                              <  1:4629208/101‑1 (MQ=255)
                                                      gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGc                                             <  1:3065265/101‑1 (MQ=255)
                                                        tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCTGGATTTTTATGGCTAACAGAAAACTCC.GCCCTTCACCtcaca                                            >  1:3283882/1‑97 (MQ=9)
                                                          cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATa                                         <  2:3397376/101‑1 (MQ=255)
                                                          cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATa                                         >  1:2294734/1‑101 (MQ=255)
                                                           gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                        <  1:4223937/101‑1 (MQ=255)
                                                             cAGCCGTCGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                      <  2:3681062/101‑1 (MQ=255)
                                                             cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                      <  2:4051915/101‑1 (MQ=255)
                                                             cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                      <  1:3849642/101‑1 (MQ=255)
                                                              aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                     >  2:2922739/1‑101 (MQ=255)
                                                                   gcgggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                               <  2:557275/100‑1 (MQ=255)
                                                                     gggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                             <  2:1609416/101‑1 (MQ=255)
                                                                       gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                           <  2:416160/101‑1 (MQ=255)
                                                                       gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                           <  2:3253867/101‑1 (MQ=255)
                                                                       gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                           <  1:848624/101‑1 (MQ=255)
                                                                        ccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                          <  2:3784874/101‑1 (MQ=255)
                                                                         cgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                         >  1:663736/1‑101 (MQ=255)
                                                                         acggacggggggCTCT.G.CCC.CCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGC.AAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                        >  2:4309734/7‑99 (MQ=2)
                                                                          ggggAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                        <  2:3648148/101‑1 (MQ=255)
                                                                          gggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                        >  2:2106101/1‑101 (MQ=255)
                                                                            gcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                      <  2:3891332/101‑1 (MQ=255)
                                                                            gacggggggCGCT.G.CCC.CCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGC.AAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaattg                     <  1:3012753/98‑6 (MQ=9)
                                                                                    gcgcT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc              >  1:3890433/1‑101 (MQ=255)
                                                                                        ctgcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg           >  1:2935175/5‑101 (MQ=255)
                                                                                         tgcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc          >  1:4439373/4‑101 (MQ=255)
                                                                                          gcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt         >  2:1957939/3‑101 (MQ=255)
                                                                                          gcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt         <  1:1021295/99‑1 (MQ=255)
                                                                                           cccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc        <  2:10326/100‑1 (MQ=255)
                                                                                           cccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc        >  1:2136297/2‑101 (MQ=255)
                                                                                            ccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa       >  2:1376364/1‑101 (MQ=255)
                                                                                            ccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa       <  1:1724979/101‑1 (MQ=255)
                                                                                             cc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa      >  2:4560387/1‑101 (MQ=255)
                                                                                             cc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa      <  1:1097123/101‑1 (MQ=255)
                                                                                             cc.ccGGCCTGCGGGCT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa      <  1:2431716/101‑1 (MQ=255)
                                                                                              c.ccGTCCTGCGGCCT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc     <  2:909856/101‑1 (MQ=255)
                                                                                                ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc    >  2:122683/1‑101 (MQ=255)
                                                                                                ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc    <  1:4078692/101‑1 (MQ=255)
                                                                                                ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc    >  1:3580067/1‑101 (MQ=255)
                                                                                                ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAAcc    >  2:1020723/1‑101 (MQ=255)
                                                                                                  |                                                                                                        
ACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGC.AAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGA  >  NC_014034/1734752‑1734947

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG