Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,849 C→G 4.0% intergenic (‑102/+91) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8490C→G 4.0% 9.9 ‑0.8 ‑0.0197intergenic (‑102/+91)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (84/105):  total (89/108)
Fisher's exact test strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.77e-01

Only 100 of 200 total aligned reads displayed.

ACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAG  >  NC_014034/1734752‑1734949
                                                                                                   |                                                                                                        
  cTCGGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGc                                                                                                      <  2:260627/101‑1 (MQ=255)
    ccGGGAAATGGCGCATCCTAACAGCCGGGGCGGGCAAGCCAAAAGCTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCATGCgg                                                                                                    <  1:318334/101‑1 (MQ=37)
      ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGAc                                                                                                  <  2:4610887/101‑1 (MQ=255)
      ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGAc                                                                                                  >  2:1021212/1‑101 (MQ=255)
      ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGAc                                                                                                  <  2:2230495/101‑1 (MQ=255)
         gAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTcc                                                                                               >  1:410136/1‑101 (MQ=255)
          aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTccc                                                                                              <  2:4511714/101‑1 (MQ=255)
            tGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTccccc                                                                                            >  2:1207352/1‑101 (MQ=255)
             ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTcccccc                                                                                           <  2:4404005/101‑1 (MQ=255)
             ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTcccccc                                                                                           >  1:2553774/1‑101 (MQ=255)
              gcgcCTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCg                                                                                          <  1:3933111/101‑1 (MQ=255)
               cgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCgg                                                                                         <  2:946482/101‑1 (MQ=255)
               cgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCgg                                                                                         >  1:1070417/1‑101 (MQ=255)
                gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCggg                                                                                        <  2:3905654/101‑1 (MQ=255)
                 cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCCGGa                                                                                       >  2:422033/1‑101 (MQ=255)
                   tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGata                                                                                     <  2:1457478/101‑1 (MQ=255)
                   tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGata                                                                                     <  1:3574375/101‑1 (MQ=255)
                    ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGatat                                                                                    <  1:2148178/101‑1 (MQ=255)
                    ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGatat                                                                                    >  1:808884/1‑101 (MQ=255)
                      tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATAttt                                                                                  >  2:197665/1‑101 (MQ=255)
                      caggcgcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCCTCCGCCGGCGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATAttt                                                                                  <  2:616689/96‑1 (MQ=18)
                        gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                                <  1:2365798/101‑1 (MQ=255)
                        gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                                >  1:416197/1‑101 (MQ=255)
                         cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                               >  2:3530466/1‑101 (MQ=255)
                         cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                               <  1:4425695/101‑1 (MQ=255)
                          agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                                              <  2:1078495/101‑1 (MQ=255)
                           gcCGGGGCGGGCAAGCCAAGGGCTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                                             <  1:2462901/101‑1 (MQ=21)
                           gcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                                             <  2:1882044/101‑1 (MQ=255)
                               gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                       <  1:1627934/101‑1 (MQ=255)
                               gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                       <  1:2419850/101‑1 (MQ=255)
                                ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCTCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.At                                                                      <  2:752858/101‑1 (MQ=255)
                                 gcgggcTAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATg                                                                     <  1:765635/101‑1 (MQ=255)
                                   gggcAAGCCAAAGGCTGTCGGTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                   <  1:1971221/101‑1 (MQ=18)
                                   gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                   >  2:1971383/1‑101 (MQ=255)
                                    ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaaa                                                                  >  2:681505/1‑101 (MQ=255)
                                       aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTg                                                               >  2:3435053/1‑101 (MQ=255)
                                        aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATTTTTATGGC.AAG.ATGAAACTGc                                                              <  1:2644089/101‑1 (MQ=255)
                                        aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGc                                                              <  1:3790451/101‑1 (MQ=255)
                                         gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCATGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                            <  1:1763434/101‑1 (MQ=255)
                                         gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                            <  1:3039082/101‑1 (MQ=255)
                                         gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                            <  1:1580250/101‑1 (MQ=255)
                                          ccAATGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.gg                                                           <  2:2930853/101‑1 (MQ=255)
                                            aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGcc                                                         >  2:2059384/1‑101 (MQ=255)
                                             aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCt                                                        <  1:1187209/101‑1 (MQ=255)
                                               ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCCGGGCAATTATGGC.AAGAATAAAATGGC.GGCCtc                                                       >  1:5823/1‑100 (MQ=17)
                                                gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCa                                                     <  2:910693/101‑1 (MQ=255)
                                                gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCa                                                     <  1:2282492/101‑1 (MQ=255)
                                                 cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCAt                                                    <  2:2530820/101‑1 (MQ=255)
                                                  tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCCTCAtt                                                   >  1:40369/1‑100 (MQ=255)
                                                    gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                 <  2:3512624/100‑1 (MQ=255)
                                                    aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.TTGAAACTGC.GGCCTTCATCtt                                                 <  2:4459524/101‑1 (MQ=255)
                                                    aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                 <  1:971746/101‑1 (MQ=255)
                                                    aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                 <  2:3722210/101‑1 (MQ=255)
                                                     cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                >  1:712115/1‑101 (MQ=255)
                                                     cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                <  2:2338294/101‑1 (MQ=255)
                                                      gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGc                                               <  2:1366481/101‑1 (MQ=255)
                                                       cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGcc                                              >  1:662959/1‑101 (MQ=255)
                                                        tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCTGGATTTTTATGGCTAAC.AGAAAACTCC.GCCCTTCACCtcaca                                              >  1:3283882/1‑97 (MQ=9)
                                                         gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCAt                                            <  1:1548212/101‑1 (MQ=255)
                                                          cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGTGAAATTTATGGC.AAG.ATGAAACTGC.GACCTTCATCTTGCCATa                                           >  1:436418/1‑101 (MQ=17)
                                                          cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                           >  1:474469/1‑101 (MQ=255)
                                                          cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                           <  2:3397376/101‑1 (MQ=255)
                                                           gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATaa                                          >  2:1097034/1‑101 (MQ=255)
                                                            tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATaaa                                         >  1:3719863/1‑101 (MQ=255)
                                                             cAGCCGTCGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                        <  2:3681062/101‑1 (MQ=255)
                                                             cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                        >  1:1167391/1‑101 (MQ=255)
                                                             cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                        <  2:4051915/101‑1 (MQ=255)
                                                              aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                       >  2:1428254/1‑101 (MQ=255)
                                                              aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                       >  2:2922739/1‑101 (MQ=255)
                                                                  gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                   >  1:295962/1‑101 (MQ=255)
                                                                   gcgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                 <  2:557275/100‑1 (MQ=255)
                                                                   aCGGGCAGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                 <  1:1657182/101‑1 (MQ=255)
                                                                    cgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                >  1:2105841/1‑101 (MQ=255)
                                                                     gggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                               <  2:1609416/101‑1 (MQ=255)
                                                                      ggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATACC.gggg                              >  2:1448584/1‑101 (MQ=255)
                                                                       tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                            >  1:2761741/9‑101 (MQ=2)
                                                                       tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                            >  2:18019/9‑101 (MQ=2)
                                                                       gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                             <  2:3253867/101‑1 (MQ=255)
                                                                       gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                             <  2:416160/101‑1 (MQ=255)
                                                                        ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGG.AAG.ATGAAACTGC.GGGCTTCATCTTGCCCTAAATATCCC.cggggg                            >  1:1745823/1‑99 (MQ=14)
                                                                        ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                            <  2:3784874/101‑1 (MQ=255)
                                                                        ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                            <  1:1378546/101‑1 (MQ=255)
                                                                         acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                          >  2:4309734/7‑99 (MQ=2)
                                                                         acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                          >  1:4440965/7‑99 (MQ=2)
                                                                          ggggAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                          <  2:3648148/101‑1 (MQ=255)
                                                                          gggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                          <  1:3864913/101‑1 (MQ=255)
                                                                          gggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                          >  2:2106101/1‑101 (MQ=255)
                                                                            gcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                        <  2:3891332/101‑1 (MQ=255)
                                                                             cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                       >  1:4273313/1‑101 (MQ=255)
                                                                              aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                      <  2:1640858/101‑1 (MQ=255)
                                                                               ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                     <  2:1070328/101‑1 (MQ=255)
                                                                                      cctccccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAACCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc              <  1:2334251/96‑1 (MQ=255)
                                                                                       ctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg             <  1:1207444/97‑1 (MQ=255)
                                                                                        tgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.GTGAAAATGC.GGCCTTCATCTTTCCATAAATATCCC.GGGGTGCGGGGGAGAGCCCCGc            >  1:1105222/4‑101 (MQ=18)
                                                                                         gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt           <  1:298334/99‑1 (MQ=255)
                                                                                         gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt           <  1:1021295/99‑1 (MQ=255)
                                                                                            cc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa        >  2:4560387/1‑101 (MQ=255)
                                                                                             c.ccGTCCTGCGGCCTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc       <  2:909856/101‑1 (MQ=255)
                                                                                                 gTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa    >  1:3081382/1‑101 (MQ=255)
                                                                                                   ccTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg  >  2:2004868/1‑101 (MQ=255)
                                                                                                   |                                                                                                        
ACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAG  >  NC_014034/1734752‑1734949

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG