Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,874 A→G 4.0% intergenic (‑127/+66) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8740A→G 4.0% 11.8 ‑1.2 ‑0.2198intergenic (‑127/+66)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (82/108):  total (87/111)
Fisher's exact test strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.99e-01

Only 100 of 218 total aligned reads displayed.

AGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTT  >  NC_014034/1734775‑1734974
                                                                                                      |                                                                                                      
 gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTAt                                                                                                       >  1:416197/1‑101 (MQ=255)
 gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTAt                                                                                                       <  1:2365798/101‑1 (MQ=255)
   agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATgg                                                                                                     <  2:1078495/101‑1 (MQ=255)
    gcCGGGGCGGGCAAGCCAAGGGCTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGc                                                                                                    <  1:2462901/101‑1 (MQ=21)
      cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCaa                                                                                                  >  2:393398/1‑101 (MQ=255)
       ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAg                                                                                                 >  1:2277184/1‑101 (MQ=255)
        gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGa                                                                                                >  2:580099/1‑101 (MQ=255)
          gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATg                                                                                              <  1:1570152/101‑1 (MQ=255)
          gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCCGGCTATTTTTTGGAAGATg                                                                                              >  1:56399/1‑101 (MQ=21)
           cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGa                                                                                             <  2:3657040/101‑1 (MQ=255)
            gggcAAGCCAAAGGCTGTCGGTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaa                                                                                            <  1:1971221/101‑1 (MQ=18)
            gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaa                                                                                            <  1:3516714/101‑1 (MQ=255)
             ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaaa                                                                                           <  1:3150948/101‑1 (MQ=255)
                aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                        >  2:3435053/1‑101 (MQ=255)
                aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                        >  2:4229995/1‑101 (MQ=255)
                aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGAAATTTATTGGAAGAAGaaaacg                                                                                        >  1:1604283/1‑98 (MQ=18)
                 aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATTTTTATGGCAAGATGAAACTGc                                                                                       <  1:2644089/101‑1 (MQ=255)
                  gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                     <  1:3039082/101‑1 (MQ=255)
                   ccAATGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                                    <  2:2930853/101‑1 (MQ=255)
                   ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                                    >  1:66097/1‑101 (MQ=255)
                     aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                  >  2:197414/1‑101 (MQ=255)
                      aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                 <  1:1187209/101‑1 (MQ=255)
                         gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                              <  2:910693/101‑1 (MQ=255)
                          cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCAGGATATGTATGGCAAGATGGAACTGC.GGCCTTCAt                                                                             >  2:1880036/1‑101 (MQ=18)
                           tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCCGGGAAATTTTTGGCAAAAAGAAAACGC.GGCCTTCAt                                                                             >  1:2399213/1‑101 (MQ=17)
                           tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCCTCAtt                                                                            >  1:40369/1‑100 (MQ=255)
                            gccccTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAATATGAAACTGC.GGCCTTCATCt                                                                           <  2:1116824/97‑1 (MQ=18)
                            gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                           <  1:732751/101‑1 (MQ=255)
                            gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAAAAGAAACTGC.GGGCTTCATCt                                                                           >  1:2616302/1‑101 (MQ=18)
                             aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                          <  1:3938117/101‑1 (MQ=255)
                             aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGTTGAAACTGC.GGCCTTCATCtt                                                                          <  2:4459524/101‑1 (MQ=255)
                              cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                         <  1:4629208/101‑1 (MQ=255)
                              cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                         <  2:2338294/101‑1 (MQ=255)
                               gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                        <  2:1366481/101‑1 (MQ=255)
                                 tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                      <  2:2149657/101‑1 (MQ=255)
                                   cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGTGAAATTTATGGCAAGATGAAACTGC.GACCTTCATCTTGCCATa                                                                    >  1:436418/1‑101 (MQ=17)
                                   cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                    >  1:2294734/1‑101 (MQ=255)
                                   cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                    <  2:3397376/101‑1 (MQ=255)
                                     tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                  >  1:3719863/1‑101 (MQ=255)
                                      cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                 >  2:6950/1‑101 (MQ=255)
                                      cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                 >  1:1167391/1‑101 (MQ=255)
                                      cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                 >  2:2509379/1‑101 (MQ=255)
                                       aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                >  2:1428254/1‑101 (MQ=255)
                                       aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                >  2:2922739/1‑101 (MQ=255)
                                          cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                             <  2:372355/101‑1 (MQ=255)
                                            aCGGGCAGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                          <  1:1657182/101‑1 (MQ=255)
                                              gggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.ggg                                                        >  2:35097/1‑101 (MQ=255)
                                                tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                     >  1:2761741/9‑101 (MQ=2)
                                                gccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                      <  1:848624/101‑1 (MQ=255)
                                                gccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                      <  2:416160/101‑1 (MQ=255)
                                                gccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                      <  2:3253867/101‑1 (MQ=255)
                                                gccgggcAGGAGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                      <  2:2077344/101‑1 (MQ=255)
                                                 gacggacggggggCTCT.G.CCCCCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                    <  2:675332/94‑2 (MQ=2)
                                                 ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGGAAGATGAAACTGC.GGGCTTCATCTTGCCCTAAATATCCC.cggggg                                                     >  1:1745823/1‑99 (MQ=14)
                                                 ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                     <  2:3784874/101‑1 (MQ=255)
                                                 ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                     <  1:1378546/101‑1 (MQ=255)
                                                  cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                    >  1:663736/1‑101 (MQ=255)
                                                  acggacggggggCTCT.G.CCCCCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                   <  2:4332654/95‑3 (MQ=2)
                                                   gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                   <  1:3864913/101‑1 (MQ=255)
                                                      cAGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                >  1:4273313/1‑101 (MQ=255)
                                                       aGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                               >  2:4078406/1‑101 (MQ=255)
                                                       aGGGGGGCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                               <  2:1640858/101‑1 (MQ=255)
                                                        ggggggCGCT.GCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                              <  2:1070328/101‑1 (MQ=255)
                                                                gctgccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                       <  2:3867039/96‑1 (MQ=255)
                                                                 ctgccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                      >  1:2935175/5‑101 (MQ=255)
                                                                 ctgccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                      <  1:1207444/97‑1 (MQ=255)
                                                                  tgccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                     >  1:4439373/4‑101 (MQ=255)
                                                                   gccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                    <  1:1021295/99‑1 (MQ=255)
                                                                   gccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                    >  2:1957939/3‑101 (MQ=255)
                                                                   gccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                    <  1:298334/99‑1 (MQ=255)
                                                                      ccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                 <  1:1097123/101‑1 (MQ=255)
                                                                      ccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                 >  2:4560387/1‑101 (MQ=255)
                                                                      ccccGGCCTGCGGGCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                 <  1:2431716/101‑1 (MQ=255)
                                                                       cccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc                                >  2:159375/1‑101 (MQ=255)
                                                                        ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                               >  2:122683/1‑101 (MQ=255)
                                                                          gTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                             >  1:3081382/1‑101 (MQ=255)
                                                                            ccTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                           >  2:2004868/1‑101 (MQ=255)
                                                                             cTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                          >  2:2702641/1‑101 (MQ=255)
                                                                              tGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                         <  2:3719588/101‑1 (MQ=255)
                                                                               gCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                        <  1:1149854/101‑1 (MQ=255)
                                                                                 ggACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                      >  2:773351/1‑101 (MQ=255)
                                                                                  gccT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                     <  2:451418/99‑1 (MQ=255)
                                                                                   aCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                    <  1:2077801/101‑1 (MQ=255)
                                                                                   aCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                    >  2:3647358/1‑101 (MQ=255)
                                                                                    cT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                   <  1:4407306/101‑1 (MQ=255)
                                                                                          cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg              >  1:1485187/1‑101 (MQ=255)
                                                                                           ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc             <  1:3897627/101‑1 (MQ=255)
                                                                                           ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc             <  1:3378216/101‑1 (MQ=255)
                                                                                           ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc             >  2:1031552/1‑101 (MQ=255)
                                                                                            cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg            >  2:3140917/1‑101 (MQ=255)
                                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg           >  1:3845935/1‑101 (MQ=255)
                                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg           >  2:1611064/1‑101 (MQ=255)
                                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg           >  2:908707/1‑101 (MQ=255)
                                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg           >  2:4568177/1‑101 (MQ=255)
                                                                                              ggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc          >  1:1867227/1‑101 (MQ=255)
                                                                                                 tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg       >  2:1633152/1‑101 (MQ=255)
                                                                                                 tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg       <  1:197689/101‑1 (MQ=255)
                                                                                                  atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGGCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg      >  1:2107108/1‑101 (MQ=255)
                                                                                                    ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgggt    >  1:975317/1‑99 (MQ=255)
                                                                                                      aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt  >  2:517194/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
AGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTT  >  NC_014034/1734775‑1734974

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG