Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,875 T→C 4.0% intergenic (‑128/+65) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8750T→C 4.0% 11.6 ‑0.8 ‑0.1199intergenic (‑128/+65)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (83/107):  total (88/111)
Fisher's exact test strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.29e-01

Only 100 of 221 total aligned reads displayed.

GCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTC  >  NC_014034/1734776‑1734975
                                                                                                    |                                                                                                        
gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                                                          <  1:2365798/101‑1 (MQ=255)
 cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                                         <  1:1232154/101‑1 (MQ=255)
 cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                                         <  1:4425695/101‑1 (MQ=255)
   gcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                                                                       <  2:1882044/101‑1 (MQ=255)
     cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.aa                                                                                                    <  2:1629925/101‑1 (MQ=255)
     cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.aa                                                                                                    >  2:393398/1‑101 (MQ=255)
     cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.aa                                                                                                    >  2:3336391/1‑101 (MQ=255)
      ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAg                                                                                                   >  1:2277184/1‑101 (MQ=255)
       gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                                                 >  2:580099/1‑101 (MQ=255)
       gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                                                 <  1:2761632/101‑1 (MQ=255)
       gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                                                 <  1:2419850/101‑1 (MQ=255)
        ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.At                                                                                                >  1:4334206/1‑101 (MQ=255)
         gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATg                                                                                               <  1:3516638/101‑1 (MQ=255)
         gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATg                                                                                               <  1:1570152/101‑1 (MQ=255)
          cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGa                                                                                              <  2:3657040/101‑1 (MQ=255)
           gggcAAGCCAAAGGCTGTCGGTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                                             <  1:1971221/101‑1 (MQ=18)
           gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                                             <  1:3776779/101‑1 (MQ=255)
           gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                                             >  2:1971383/1‑101 (MQ=255)
               aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTg                                                                                         >  2:4229995/1‑101 (MQ=255)
               aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTg                                                                                         >  2:3435053/1‑101 (MQ=255)
               aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTg                                                                                         <  2:1980975/101‑1 (MQ=255)
               aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAAATTTATTGG.AAG.AAGaaaacg                                                                                         >  1:1604283/1‑98 (MQ=18)
                 gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCATGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                                                      <  1:1763434/101‑1 (MQ=255)
                 gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                                                      <  1:3039082/101‑1 (MQ=255)
                    aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGcc                                                                                   >  1:4395081/1‑101 (MQ=255)
                    aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGcc                                                                                   >  2:2059384/1‑101 (MQ=255)
                     aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCt                                                                                  <  1:1187209/101‑1 (MQ=255)
                       ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGCAATTATGGC.AAGAATAAAATGGC.GGCCtc                                                                                 >  1:5823/1‑100 (MQ=17)
                        gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCa                                                                               <  2:910693/101‑1 (MQ=255)
                           gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAA.AAGAAACTGC.GGGCTTCATCt                                                                            >  1:2616302/1‑101 (MQ=18)
                            gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                                           <  2:3512624/100‑1 (MQ=255)
                             cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                                          >  1:712115/1‑101 (MQ=255)
                             cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                                          <  1:4629208/101‑1 (MQ=255)
                              gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGc                                                                         <  2:1366481/101‑1 (MQ=255)
                              gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGc                                                                         >  2:4465189/1‑101 (MQ=255)
                               cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGcc                                                                        >  1:662959/1‑101 (MQ=255)
                                tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCTGGATTTTTATGGCTAAC.AGAAAACTCC.GCCCTTCACCtcaca                                                                        >  1:3283882/1‑97 (MQ=9)
                                  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                     <  2:3397376/101‑1 (MQ=255)
                                  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                     >  1:2294734/1‑101 (MQ=255)
                                   gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                    >  2:1097034/1‑101 (MQ=255)
                                   gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                    <  1:4223937/101‑1 (MQ=255)
                                     cAGCCGACGGGCCGGGCAGGGGGGCGGGGCCCCCCGTCCGGCGGACTCCCCCCCGTTTATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                  >  2:2328859/1‑101 (MQ=17)
                                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                  >  2:6950/1‑101 (MQ=255)
                                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                  >  1:1167391/1‑101 (MQ=255)
                                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                  >  2:2509379/1‑101 (MQ=255)
                                     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                  <  1:3849642/101‑1 (MQ=255)
                                      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                 >  2:2922739/1‑101 (MQ=255)
                                      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                 >  2:1428254/1‑101 (MQ=255)
                                         cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                              >  2:4407014/1‑101 (MQ=255)
                                          gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                                             >  1:295962/1‑101 (MQ=255)
                                            cgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                          >  1:2105841/1‑101 (MQ=255)
                                             gggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                         <  2:1609416/101‑1 (MQ=255)
                                               gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                       <  1:848624/101‑1 (MQ=255)
                                               gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                       <  2:416160/101‑1 (MQ=255)
                                                ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                      <  1:1378546/101‑1 (MQ=255)
                                                 acggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                    <  2:4332654/95‑3 (MQ=2)
                                                    gcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                  <  2:3891332/101‑1 (MQ=255)
                                                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                >  2:4078406/1‑101 (MQ=255)
                                                       ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                               <  2:1070328/101‑1 (MQ=255)
                                                            gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                          >  1:3890433/1‑101 (MQ=255)
                                                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                     <  1:1021295/99‑1 (MQ=255)
                                                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                    >  1:2136297/2‑101 (MQ=255)
                                                                   cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                   <  1:1724979/101‑1 (MQ=255)
                                                                    ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                  <  1:1097123/101‑1 (MQ=255)
                                                                    ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                  >  2:4560387/1‑101 (MQ=255)
                                                                      ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                >  1:3580067/1‑101 (MQ=255)
                                                                      ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                <  1:4078692/101‑1 (MQ=255)
                                                                           cTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                           >  2:2702641/1‑101 (MQ=255)
                                                                            tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                          <  2:3719588/101‑1 (MQ=255)
                                                                            tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                          <  2:1745704/101‑1 (MQ=255)
                                                                            tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                          <  2:2105691/101‑1 (MQ=255)
                                                                             gCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                         <  1:1149854/101‑1 (MQ=255)
                                                                               ggACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                       >  2:773351/1‑101 (MQ=255)
                                                                                 aCTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                     <  1:393435/101‑1 (MQ=255)
                                                                                 aCTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                     >  2:3647358/1‑101 (MQ=255)
                                                                                  cTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                    <  2:2951285/101‑1 (MQ=255)
                                                                                    ccccccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                  <  1:2826345/101‑1 (MQ=255)
                                                                                     cccccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                 <  1:24507/101‑1 (MQ=255)
                                                                                     cccccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                 >  2:1247369/1‑101 (MQ=255)
                                                                                       cccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg               >  1:1485187/1‑101 (MQ=255)
                                                                                       cccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg               <  1:2509553/101‑1 (MQ=255)
                                                                                        ccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc              <  2:3762212/101‑1 (MQ=255)
                                                                                        ccGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc              >  2:1031552/1‑101 (MQ=255)
                                                                                          gggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg            >  2:4568177/1‑101 (MQ=255)
                                                                                          gggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg            >  2:908707/1‑101 (MQ=255)
                                                                                           ggATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc           >  1:1867227/1‑101 (MQ=255)
                                                                                             atatTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc         >  2:316229/1‑101 (MQ=255)
                                                                                             atatTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc         >  1:497080/1‑101 (MQ=255)
                                                                                              tatTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCTCGCTCAACCGAAGATGACAAGATCCGCGGCCCg        >  1:4031460/1‑101 (MQ=255)
                                                                                              tatTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg        <  1:197689/101‑1 (MQ=255)
                                                                                               atTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg       >  1:3142166/1‑101 (MQ=255)
                                                                                               atTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg       >  2:1636907/1‑101 (MQ=255)
                                                                                                tttATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt      >  2:3824198/1‑101 (MQ=255)
                                                                                                tttATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt      >  1:912657/1‑101 (MQ=255)
                                                                                                 ttATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgggt     >  1:975317/1‑99 (MQ=255)
                                                                                                 ttATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt     <  2:662897/101‑1 (MQ=255)
                                                                                                  tATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt    <  1:2987816/101‑1 (MQ=255)
                                                                                                   aTGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt   <  2:1163157/101‑1 (MQ=255)
                                                                                                   aTGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt   >  2:517194/1‑101 (MQ=255)
                                                                                                    tGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc  <  2:2373514/101‑1 (MQ=255)
                                                                                                    |                                                                                                        
GCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTC  >  NC_014034/1734776‑1734975

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG