Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,887 A→G 4.2% intergenic (‑140/+53) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8870A→G 4.2% 10.6 ‑0.3 ‑0.1189intergenic (‑140/+53)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (91/88):  total (96/93)
Fisher's exact test strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.26e-01

Only 100 of 213 total aligned reads displayed.

GGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAA  >  NC_014034/1734788‑1734987
                                                                                                     |                                                                                                      
ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaaa                                                                                                        <  1:3150948/101‑1 (MQ=255)
  cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGCCCTTCGGACT.CCCCCCGGGAAATTTATGGCAAGATGaaaat                                                                                                      >  2:4647120/1‑99 (MQ=18)
   aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                     >  2:4229995/1‑101 (MQ=255)
   aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                     >  1:1403475/1‑101 (MQ=255)
   aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                     <  2:1980975/101‑1 (MQ=255)
    aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATTTTTATGGCAAGATGAAACTGc                                                                                                    <  1:2644089/101‑1 (MQ=255)
     gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                  <  1:3039082/101‑1 (MQ=255)
     gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                  <  1:1580250/101‑1 (MQ=255)
        aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                               >  2:2059384/1‑101 (MQ=255)
        aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                               >  1:4395081/1‑101 (MQ=255)
            gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                           <  2:910693/101‑1 (MQ=255)
              tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGGAAATTTTTGGCAAAAAGAAAACGC.GGCCTTCAt                                                                                          >  1:2399213/1‑101 (MQ=17)
               gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAAAAGAAACTGC.GGGCTTCATCt                                                                                        >  1:2616302/1‑101 (MQ=18)
                gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                       <  2:3512624/100‑1 (MQ=255)
                aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                       <  2:3722210/101‑1 (MQ=255)
                aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                       <  1:971746/101‑1 (MQ=255)
                 cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                      <  1:4629208/101‑1 (MQ=255)
                 cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                      <  2:2338294/101‑1 (MQ=255)
                 cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                      >  1:712115/1‑101 (MQ=255)
                  gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                     >  2:4465189/1‑101 (MQ=255)
                   cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                                                                                    >  1:662959/1‑101 (MQ=255)
                    tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                   <  2:2149657/101‑1 (MQ=255)
                      cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                 <  2:3397376/101‑1 (MQ=255)
                       gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                >  2:1097034/1‑101 (MQ=255)
                        tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                               >  1:3719863/1‑101 (MQ=255)
                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                              >  1:1167391/1‑101 (MQ=255)
                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                              >  2:2509379/1‑101 (MQ=255)
                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                              >  2:6950/1‑101 (MQ=255)
                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                              <  2:4051915/101‑1 (MQ=255)
                             cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                          >  2:4407014/1‑101 (MQ=255)
                               gcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                       <  2:557275/100‑1 (MQ=255)
                               aCGGGCAGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                       <  1:1657182/101‑1 (MQ=255)
                                  ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATACC.gggg                                                                    >  2:1448584/1‑101 (MQ=255)
                                   tgacggacggggggCTCTG.CCCCCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                  >  2:18019/9‑101 (MQ=2)
                                   gccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                   <  1:848624/101‑1 (MQ=255)
                                    gacggacggggggCTCTG.CCCCCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                 <  2:675332/94‑2 (MQ=2)
                                    ccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGGAAGATGAAACTGC.GGGCTTCATCTTGCCCTAAATATCCC.cggggg                                                                  >  1:1745823/1‑99 (MQ=14)
                                      ggggAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                <  2:3648148/101‑1 (MQ=255)
                                      gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                >  2:2106101/1‑101 (MQ=255)
                                      gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                <  1:3864913/101‑1 (MQ=255)
                                        gcAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                              <  2:3891332/101‑1 (MQ=255)
                                         cAGGGGGGCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                             >  1:4273313/1‑101 (MQ=255)
                                           ggggggCGCTGCCCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                           <  2:1070328/101‑1 (MQ=255)
                                                  cctcccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAACCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                    <  1:2334251/96‑1 (MQ=255)
                                                   ctgccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                   >  1:2935175/5‑101 (MQ=255)
                                                    tgccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                  >  1:4439373/4‑101 (MQ=255)
                                                     gccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                 >  2:1957939/3‑101 (MQ=255)
                                                     gccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                 <  1:1021295/99‑1 (MQ=255)
                                                     gccccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                 <  1:298334/99‑1 (MQ=255)
                                                       cccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                               >  2:1376364/1‑101 (MQ=255)
                                                        ccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                              >  2:4560387/1‑101 (MQ=255)
                                                        ccccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                              <  1:1097123/101‑1 (MQ=255)
                                                         cccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc                                             >  2:159375/1‑101 (MQ=255)
                                                          ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                            <  1:4078692/101‑1 (MQ=255)
                                                          ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAAcc                                            >  2:1020723/1‑101 (MQ=255)
                                                            gTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                          >  1:3081382/1‑101 (MQ=255)
                                                               cTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                       <  1:1192006/101‑1 (MQ=255)
                                                                tGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                      <  2:1745704/101‑1 (MQ=255)
                                                                     aCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                 <  1:393435/101‑1 (MQ=255)
                                                                     aCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                 <  1:2077801/101‑1 (MQ=255)
                                                                      cT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                <  2:2951285/101‑1 (MQ=255)
                                                                         ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                              >  2:4093953/1‑101 (MQ=255)
                                                                         ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                              <  1:1971518/101‑1 (MQ=255)
                                                                          cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                             <  1:24507/101‑1 (MQ=255)
                                                                          cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                             >  2:1247369/1‑101 (MQ=255)
                                                                             ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                          <  2:3762212/101‑1 (MQ=255)
                                                                             ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                          <  1:3378216/101‑1 (MQ=255)
                                                                               gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                        >  1:3845935/1‑101 (MQ=255)
                                                                               gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                        >  2:1611064/1‑101 (MQ=255)
                                                                               gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                        >  2:908707/1‑101 (MQ=255)
                                                                               gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                        >  2:4568177/1‑101 (MQ=255)
                                                                                  atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                     >  1:497080/1‑101 (MQ=255)
                                                                                  atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                     <  1:2169977/101‑1 (MQ=255)
                                                                                  atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                     >  2:316229/1‑101 (MQ=255)
                                                                                   tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCTCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                    >  1:4031460/1‑101 (MQ=255)
                                                                                   tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                    >  2:2374884/1‑101 (MQ=255)
                                                                                    atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGGCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                   >  1:2107108/1‑101 (MQ=255)
                                                                                    atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                   >  1:1843917/1‑101 (MQ=255)
                                                                                    atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                   >  2:1636907/1‑101 (MQ=255)
                                                                                      ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                 <  2:662897/101‑1 (MQ=255)
                                                                                       tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                >  1:1369339/1‑101 (MQ=255)
                                                                                       tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                >  2:3442028/1‑101 (MQ=255)
                                                                                        aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt               <  2:1163157/101‑1 (MQ=255)
                                                                                         tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc              <  1:910413/101‑1 (MQ=255)
                                                                                          ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg             >  2:2917386/1‑101 (MQ=255)
                                                                                           gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc            >  2:4155559/1‑101 (MQ=255)
                                                                                            cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt           <  1:1448679/101‑1 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg          >  2:3809374/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg          <  1:422070/101‑1 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg          >  2:4340347/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg          >  2:2557224/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg          >  2:271040/1‑101 (MQ=255)
                                                                                                 tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga      <  2:4317973/101‑1 (MQ=255)
                                                                                                  gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg     >  2:4514987/1‑101 (MQ=255)
                                                                                                  gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg     >  2:3839722/1‑101 (MQ=255)
                                                                                                   aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc    >  2:3285496/1‑101 (MQ=255)
                                                                                                    aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa   <  1:1114439/101‑1 (MQ=255)
                                                                                                     aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa  >  1:2781664/1‑101 (MQ=255)
                                                                                                     aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa  <  1:594786/101‑1 (MQ=255)
                                                                                                     aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa  <  2:1350240/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
GGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAA  >  NC_014034/1734788‑1734987

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG