Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,892 G→T 4.2% intergenic (‑145/+48) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8920G→T 4.2% 11.8 ‑0.3 ‑0.2190intergenic (‑145/+48)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (92/89):  total (98/92)
Fisher's exact test strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.31e-01

Only 100 of 210 total aligned reads displayed.

GCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCC  >  NC_014034/1734793‑1734992
                                                                                                      |                                                                                                     
gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                       <  1:1580250/101‑1 (MQ=255)
   aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                                    >  2:197414/1‑101 (MQ=255)
       gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                                <  2:910693/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCAGGATATGTATGGCAAGATGGAACTGC.GGCCTTCAt                                                                                               >  2:1880036/1‑101 (MQ=18)
         tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCCTCAtt                                                                                              >  1:40369/1‑100 (MQ=255)
          gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                             <  1:732751/101‑1 (MQ=255)
           gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                            <  2:3512624/100‑1 (MQ=255)
           aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                            <  1:3938117/101‑1 (MQ=255)
            cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                           <  2:2338294/101‑1 (MQ=255)
             gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                          >  2:4465189/1‑101 (MQ=255)
             gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                          <  2:1366481/101‑1 (MQ=255)
             gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                          <  1:3065265/101‑1 (MQ=255)
               tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                        <  2:2149657/101‑1 (MQ=255)
                 cGTCAGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                      <  2:456167/101‑1 (MQ=255)
                 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGTGAAATTTATGGCAAGATGAAACTGC.GACCTTCATCTTGCCATa                                                                                      >  1:436418/1‑101 (MQ=17)
                 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                      >  1:2294734/1‑101 (MQ=255)
                  gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                     >  2:1097034/1‑101 (MQ=255)
                  gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                     <  1:4223937/101‑1 (MQ=255)
                   tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                    >  1:3719863/1‑101 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   <  1:3849642/101‑1 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   >  2:2509379/1‑101 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   >  1:1167391/1‑101 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   <  2:4051915/101‑1 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   >  2:6950/1‑101 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGA.GGCCTTCATATTGCCATAAat                                                                                   >  2:4527879/1‑101 (MQ=17)
                     aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                  >  2:2922739/1‑101 (MQ=255)
                        cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                               >  2:4407014/1‑101 (MQ=255)
                        cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                               <  2:372355/101‑1 (MQ=255)
                            gggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                          <  2:1609416/101‑1 (MQ=255)
                             ggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATACC.gggg                                                                         >  2:1448584/1‑101 (MQ=255)
                              gccgggcAGGAGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                        <  2:2077344/101‑1 (MQ=255)
                               ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                       <  2:3784874/101‑1 (MQ=255)
                                cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                      >  1:663736/1‑101 (MQ=255)
                                acggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                     >  1:4440965/7‑99 (MQ=2)
                                 ggggAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                     <  2:3648148/101‑1 (MQ=255)
                                 gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                     >  2:2106101/1‑101 (MQ=255)
                                   gcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                                   <  2:3891332/101‑1 (MQ=255)
                                   gacggggggCGCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaattg                                                                  <  1:3012753/98‑6 (MQ=9)
                                     aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                 <  2:1640858/101‑1 (MQ=255)
                                      ggggggCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                <  2:1070328/101‑1 (MQ=255)
                                           gcgcT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                           >  1:3890433/1‑101 (MQ=255)
                                            cgcT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATAGTTATAGTAAGCTGCAACTAG.CGCCCTCAACTTGCCCTGAATATGCC.GGGGGGTGGGGTAGAGccc                                                          >  2:4166224/1‑101 (MQ=2)
                                               ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                        >  1:2935175/5‑101 (MQ=255)
                                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                      <  1:1021295/99‑1 (MQ=255)
                                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                      <  1:298334/99‑1 (MQ=255)
                                                   cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                    <  1:1724979/101‑1 (MQ=255)
                                                    ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                   <  1:1097123/101‑1 (MQ=255)
                                                    ccccGGCCTGCGGGCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                   <  1:2431716/101‑1 (MQ=255)
                                                           cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                            <  1:1192006/101‑1 (MQ=255)
                                                            tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                           <  2:3719588/101‑1 (MQ=255)
                                                                 gcTCCCCCCGGGATATTTAGGGCAAGATGAACCTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                      <  1:2380043/100‑1 (MQ=255)
                                                                 aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                      <  1:2077801/101‑1 (MQ=255)
                                                                  cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                     <  2:2951285/101‑1 (MQ=255)
                                                                  cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                     <  1:4407306/101‑1 (MQ=255)
                                                                    ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                   >  2:4093953/1‑101 (MQ=255)
                                                                    ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                   <  1:1971518/101‑1 (MQ=255)
                                                                    ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                   <  1:2826345/101‑1 (MQ=255)
                                                                     cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                  >  2:1247369/1‑101 (MQ=255)
                                                                       cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                                >  1:1485187/1‑101 (MQ=255)
                                                                       cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                                <  1:2509553/101‑1 (MQ=255)
                                                                        ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                               <  1:3378216/101‑1 (MQ=255)
                                                                        ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                               >  2:1031552/1‑101 (MQ=255)
                                                                         cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                              >  2:594725/1‑101 (MQ=255)
                                                                          gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                             >  2:908707/1‑101 (MQ=255)
                                                                          gggATATTTATGGCAAGATGAAACTGC.GGCCATCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCAAAGATGACAAGATCCGCgg                             >  1:2629682/1‑101 (MQ=255)
                                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                          >  2:316229/1‑101 (MQ=255)
                                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                          >  1:497080/1‑101 (MQ=255)
                                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                          <  1:2169977/101‑1 (MQ=255)
                                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                         >  2:2374884/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                         >  2:1633152/1‑101 (MQ=255)
                                                                               atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGGCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                        >  1:2107108/1‑101 (MQ=255)
                                                                               atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                        >  1:1843917/1‑101 (MQ=255)
                                                                               atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                        >  1:3142166/1‑101 (MQ=255)
                                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                       >  1:912657/1‑101 (MQ=255)
                                                                                 ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgggt                      >  1:975317/1‑99 (MQ=255)
                                                                                  tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                     >  1:1369339/1‑101 (MQ=255)
                                                                                  tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                     >  2:3442028/1‑101 (MQ=255)
                                                                                   aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                    <  2:1163157/101‑1 (MQ=255)
                                                                                   aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                    >  2:517194/1‑101 (MQ=255)
                                                                                    tGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                   >  2:3395251/1‑101 (MQ=255)
                                                                                      gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                 >  2:151632/1‑101 (MQ=255)
                                                                                      gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                 >  2:4155559/1‑101 (MQ=255)
                                                                                        gggATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGCGCGGGGCAGAGCCCCGCTCAACCGCAGATGACAAGATCCGCGGCCCGGTTTTAGCtg               >  2:4571734/3‑101 (MQ=255)
                                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg               >  2:4340347/1‑101 (MQ=255)
                                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg               >  2:2557224/1‑101 (MQ=255)
                                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg               >  2:271040/1‑101 (MQ=255)
                                                                                        aaGATGAAACTGC.CGCCTTCCTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg               <  1:4647421/101‑1 (MQ=255)
                                                                                         aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga              >  1:907959/1‑101 (MQ=255)
                                                                                           aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg            <  2:1753445/101‑1 (MQ=255)
                                                                                            tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga           <  2:4317973/101‑1 (MQ=255)
                                                                                            tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga           >  1:4166847/1‑101 (MQ=255)
                                                                                             gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg          >  2:3839722/1‑101 (MQ=255)
                                                                                              aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc         >  2:3285496/1‑101 (MQ=255)
                                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa        <  1:1114439/101‑1 (MQ=255)
                                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa        <  2:663674/101‑1 (MQ=255)
                                                                                                aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa       <  1:594786/101‑1 (MQ=255)
                                                                                                aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa       >  1:1409056/1‑101 (MQ=255)
                                                                                                 cTGC.AGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg      >  2:4626156/1‑101 (MQ=255)
                                                                                                  tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc     >  2:284747/1‑101 (MQ=255)
                                                                                                      ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc  >  2:1398747/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCC  >  NC_014034/1734793‑1734992

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG