Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,894 C→A 4.2% intergenic (‑147/+46) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8940C→A 4.2% 12.0 ‑0.3 ‑0.1192intergenic (‑147/+46)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (93/91):  total (98/94)
Fisher's exact test strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.34e-01

Only 100 of 207 total aligned reads displayed.

AAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCC  >  NC_014034/1734796‑1734993
                                                                                                     |                                                                                                    
    gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCa                                                                                                 <  2:910693/101‑1 (MQ=255)
      tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCCTCAtt                                                                                               >  1:40369/1‑100 (MQ=255)
       gccccTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAATATGAAACTGC.GGCCTTCATCt                                                                                              <  2:1116824/97‑1 (MQ=18)
       gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                              <  1:732751/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                             <  1:3938117/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGTTGAAACTGC.GGCCTTCATCtt                                                                                             <  2:4459524/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                             <  2:3722210/101‑1 (MQ=255)
        aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                             <  1:971746/101‑1 (MQ=255)
         cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                            <  2:2338294/101‑1 (MQ=255)
          gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                           <  2:1366481/101‑1 (MQ=255)
          gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                           <  1:3065265/101‑1 (MQ=255)
            tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                         <  2:2149657/101‑1 (MQ=255)
             gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                        <  1:1548212/101‑1 (MQ=255)
              cGTCAGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                       <  2:456167/101‑1 (MQ=255)
              cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGTGAAATTTATGGCAAGATGAAACTGC.GACCTTCATCTTGCCATa                                                                                       >  1:436418/1‑101 (MQ=17)
              cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                       <  2:3397376/101‑1 (MQ=255)
              cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                       >  1:2294734/1‑101 (MQ=255)
               gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                      <  1:4223937/101‑1 (MQ=255)
                tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                     >  1:3719863/1‑101 (MQ=255)
                 cAGCCGTCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                    <  2:3681062/101‑1 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                    >  2:2509379/1‑101 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                    <  1:3849642/101‑1 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                    <  2:4051915/101‑1 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                    >  1:1167391/1‑101 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                    >  2:6950/1‑101 (MQ=255)
                 cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGA.GGCCTTCATATTGCCATAAat                                                                                    >  2:4527879/1‑101 (MQ=17)
                  aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                   >  2:2922739/1‑101 (MQ=255)
                      gACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                                                               >  1:295962/1‑101 (MQ=255)
                         gggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.ggg                                                                           >  2:35097/1‑101 (MQ=255)
                         gggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                           <  2:1609416/101‑1 (MQ=255)
                          ggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATACC.gggg                                                                          >  2:1448584/1‑101 (MQ=255)
                           gccgggcAGGAGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                         <  2:2077344/101‑1 (MQ=255)
                            ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                        <  1:1378546/101‑1 (MQ=255)
                             cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                       >  1:663736/1‑101 (MQ=255)
                             acggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                      <  2:4332654/95‑3 (MQ=2)
                             acggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                      >  1:4440965/7‑99 (MQ=2)
                              gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                      >  2:2106101/1‑101 (MQ=255)
                                gcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                                    <  2:3891332/101‑1 (MQ=255)
                                  aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                  <  2:1640858/101‑1 (MQ=255)
                                         cgcT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATAGTTATAGTAAGCTGCAACTAG.CGCCCTCAACTTGCCCTGAATATGCC.GGGGGGTGGGGTAGAGccc                                                           >  2:4166224/1‑101 (MQ=2)
                                              gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                       <  1:1021295/99‑1 (MQ=255)
                                              gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                       >  2:1957939/3‑101 (MQ=255)
                                               ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                      <  2:10326/100‑1 (MQ=255)
                                                cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                     <  1:1724979/101‑1 (MQ=255)
                                                 ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                    <  1:1097123/101‑1 (MQ=255)
                                                 ccccGGCCTGCGGGCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                    <  1:2431716/101‑1 (MQ=255)
                                                   ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                  >  2:122683/1‑101 (MQ=255)
                                                   ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAAcc                                                  >  2:1020723/1‑101 (MQ=255)
                                                       ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                              >  2:2004868/1‑101 (MQ=255)
                                                        cTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                             <  1:1192006/101‑1 (MQ=255)
                                                             gccTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                        <  2:451418/99‑1 (MQ=255)
                                                              gcTCCCCCCGGGATATTTAGGGCAAGATGAACCTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                       <  1:2380043/100‑1 (MQ=255)
                                                              aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                       >  2:3647358/1‑101 (MQ=255)
                                                              aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                       <  1:2077801/101‑1 (MQ=255)
                                                               cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                      <  1:4407306/101‑1 (MQ=255)
                                                               cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                      <  2:2951285/101‑1 (MQ=255)
                                                                 ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                    >  2:4093953/1‑101 (MQ=255)
                                                                 ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                    <  1:1971518/101‑1 (MQ=255)
                                                                    cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                                 <  1:2509553/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                <  2:4373336/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                <  1:3378216/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                <  2:3762212/101‑1 (MQ=255)
                                                                     ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                                <  1:3897627/101‑1 (MQ=255)
                                                                      cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                               >  2:594725/1‑101 (MQ=255)
                                                                       gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                              >  2:908707/1‑101 (MQ=255)
                                                                       gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATACGCgg                              >  2:1275148/1‑101 (MQ=255)
                                                                       gggATATTTATGGCAAGATGAAACTGC.GGCCATCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCAAAGATGACAAGATCCGCgg                              >  1:2629682/1‑101 (MQ=255)
                                                                          atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                           <  1:2169977/101‑1 (MQ=255)
                                                                          atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                           >  2:316229/1‑101 (MQ=255)
                                                                          atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                           >  1:497080/1‑101 (MQ=255)
                                                                           tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                          >  2:2374884/1‑101 (MQ=255)
                                                                           tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                          >  2:1633152/1‑101 (MQ=255)
                                                                            atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGGCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                         >  1:2107108/1‑101 (MQ=255)
                                                                            atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                         >  1:3142166/1‑101 (MQ=255)
                                                                            atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                         >  1:1843917/1‑101 (MQ=255)
                                                                               tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                      <  1:2987816/101‑1 (MQ=255)
                                                                                aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                     >  2:517194/1‑101 (MQ=255)
                                                                                   gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                  >  2:151632/1‑101 (MQ=255)
                                                                                   gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                  >  2:4155559/1‑101 (MQ=255)
                                                                                    cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                 <  1:1448679/101‑1 (MQ=255)
                                                                                     gggATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGCGCGGGGCAGAGCCCCGCTCAACCGCAGATGACAAGATCCGCGGCCCGGTTTTAGCtg                >  2:4571734/3‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                >  2:3809374/1‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                >  2:271040/1‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                >  2:2557224/1‑101 (MQ=255)
                                                                                     aaGATGAAACTGC.CGCCTTCCTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg                <  1:4647421/101‑1 (MQ=255)
                                                                                      aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga               >  1:907959/1‑101 (MQ=255)
                                                                                        aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg             <  2:1753445/101‑1 (MQ=255)
                                                                                         tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga            >  1:4166847/1‑101 (MQ=255)
                                                                                          gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg           >  2:3839722/1‑101 (MQ=255)
                                                                                            aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa         <  2:663674/101‑1 (MQ=255)
                                                                                            aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa         <  1:1114439/101‑1 (MQ=255)
                                                                                             aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa        <  1:594786/101‑1 (MQ=255)
                                                                                             aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa        >  1:2781664/1‑101 (MQ=255)
                                                                                              cTGC.AGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg       >  2:4626156/1‑101 (MQ=255)
                                                                                               tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc      >  2:284747/1‑101 (MQ=255)
                                                                                               tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGGTTTCGCTGATGAGCAAgc      >  2:1079233/1‑101 (MQ=255)
                                                                                                gC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg     <  2:337959/101‑1 (MQ=255)
                                                                                                   ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc   <  1:2922968/101‑1 (MQ=255)
                                                                                                   ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc   >  2:1398747/1‑101 (MQ=255)
                                                                                                   cgCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc   <  1:122697/100‑1 (MQ=255)
                                                                                                     |                                                                                                    
AAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCC  >  NC_014034/1734796‑1734993

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG